Plasmonic gold nanoparticles as multifaceted probe for tissue imaging

2019 ◽  
Vol 55 (19) ◽  
pp. 2761-2764 ◽  
Author(s):  
Yu-Hong Cheng ◽  
Toby Siu-Chung Tam ◽  
Siu-Leung Chau ◽  
Samuel Kin-Man Lai ◽  
Ho-Wai Tang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Imaging Techniques ◽  
Mass Spectrometric ◽  
Tissue Imaging ◽  
High Quality

Gold nanoparticles as a sensitive probe for versatile tissue imaging techniques forming high quality chromogenic, fluorescence, and mass spectrometric images.

scholarly journals Optimization of X-ray Investigations in Dentistry Using Optical Coherence Tomography

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4554
Author(s):  
Ralph-Alexandru Erdelyi ◽  
Virgil-Florin Duma ◽  
Cosmin Sinescu ◽  
George Mihai Dobre ◽  
Adrian Bradu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Radiation Dose ◽  
Imaging Techniques ◽  
Image Resolution ◽  
Optical Coherence ◽  
X Rays ◽  
High Quality ◽  
X Ray

The most common imaging technique for dental diagnoses and treatment monitoring is X-ray imaging, which evolved from the first intraoral radiographs to high-quality three-dimensional (3D) Cone Beam Computed Tomography (CBCT). Other imaging techniques have shown potential, such as Optical Coherence Tomography (OCT). We have recently reported on the boundaries of these two types of techniques, regarding. the dental fields where each one is more appropriate or where they should be both used. The aim of the present study is to explore the unique capabilities of the OCT technique to optimize X-ray units imaging (i.e., in terms of image resolution, radiation dose, or contrast). Two types of commercially available and widely used X-ray units are considered. To adjust their parameters, a protocol is developed to employ OCT images of dental conditions that are documented on high (i.e., less than 10 μm) resolution OCT images (both B-scans/cross sections and 3D reconstructions) but are hardly identified on the 200 to 75 μm resolution panoramic or CBCT radiographs. The optimized calibration of the X-ray unit includes choosing appropriate values for the anode voltage and current intensity of the X-ray tube, as well as the patient’s positioning, in order to reach the highest possible X-rays resolution at a radiation dose that is safe for the patient. The optimization protocol is developed in vitro on OCT images of extracted teeth and is further applied in vivo for each type of dental investigation. Optimized radiographic results are compared with un-optimized previously performed radiographs. Also, we show that OCT can permit a rigorous comparison between two (types of) X-ray units. In conclusion, high-quality dental images are possible using low radiation doses if an optimized protocol, developed using OCT, is applied for each type of dental investigation. Also, there are situations when the X-ray technology has drawbacks for dental diagnosis or treatment assessment. In such situations, OCT proves capable to provide qualitative images.

MO430MASS-SPECTROMETRIC IDENTIFICATION OF POST-TRANSLATIONAL GUANIDINYLATED PROTEINS IN THE CONTEXT OF SYSTEMIC LUPUS ERYTHEMATOSUS

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Juliane Hermann ◽  
Ute Raffetseder ◽  
Michaela Lellig ◽  
Joachim Jankowski ◽  
Vera Jankowski
Keyword(s):  
Mass Spectrometry ◽  
Systemic Lupus Erythematosus ◽  
High Resolution ◽  
Lupus Erythematosus ◽  
Imaging Techniques ◽  
Mass Spectrometric ◽  
Mass Spectrometric Imaging ◽  
Systemic Lupus ◽  
Notch 3 ◽  
Resolution Mass

Abstract Background and Aims With continuous identification of post-translational modified isoforms of proteins, it is becoming increasingly clear that post-translational modifications limit or modify the biological functions of native proteins are majorly involved in development of various chronic disease. This is mostly due to technically advanced molecular identification and quantification methods, mainly based on mass spectrometry. Mass spectrometry has become one of the most powerful tools for the identification of lipids. Method In this study, we used sophisticated high-resolution mass-spectrometric methods to analyze the soluble ligand of receptor Notch-3, namely the Y-box protein (YB)-1, in serum from systemic lupus erythematosus (SLE) patients. In addition, kidneys of lupus-prone (MRL.lpr) mice were analyzed by mass-spectrometric imaging techniques to identify the underlying pathomechanisms. Serum YB-1 was isolated by chromatographic methods, afterwards digested by trypsin and analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The kidneys were fixed in paraffin, then kidney sections were deparaffinized, tryptic digested and analyzed by mass-spectrometric imaging techniques. Mass-spectrometry of extracellular YB-1 in SLE patient serum revealed post-translational guanidinylation of two lysine’s within the highly conserved cold shock domain (CSD) of the YB-1 protein (YB-1-2G). Patients with increased disease activity and those with active renal involvement (lupus nephritis, LN) had a higher degree of dual-guanidinylation within the CSD. Of note, at least one of these modifications was present in all analyzed LN patients, whereas single-guanidinylated YB-1 was present in only one and double modification in none of the control individuals. Mass-spectrometric imaging analyses specifically localized YB-1-2G and increases Notch-3 expression in kidney sections from MRL.lpr mice. Results The data from this study clearly demonstrate the high potential of high-resolution mass spectrometric methods as well as mass spectrometric imaging techniques to identify pathomechanisms of diseases like SLE/LN.

scholarly journals Multiphoton Microscopy for Ophthalmic Imaging

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Emily A. Gibson ◽  
Omid Masihzadeh ◽  
Tim C. Lei ◽  
David A. Ammar ◽  
Malik Y. Kahook
Keyword(s):  
Harmonic Generation ◽  
Second Harmonic ◽  
Imaging Techniques ◽  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Third Harmonic ◽  
Two Photon ◽  
Disease Mechanisms ◽  
Ophthalmic Imaging ◽  
Anti Stokes

We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.

The Effect of Gold Nanoparticles Combined with High-Quality Nursing in Patients with Myocardial Infarction

2021 ◽  
Vol 11 (1) ◽  
pp. 165-170
Author(s):  
Shasha Lv ◽  
Ling Yang ◽  
Ruifei Wu ◽  
Xiaoxing Feng
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Gold Nanoparticles ◽  
Molecular Analysis ◽  
Healthy Subjects ◽  
Control Group ◽  
Observation Group ◽  
High Quality ◽  
Nano Gold

This study was established to study the effect of gold nanoparticles combined with high-quality nursing in patients with acute myocardial infarction (AMI). Under the condition of reflux, a nano gold strip was prepared. One hundred patients with AMI who were treated from April to September 2018 at our Hospital were selected for analysis, divided equally between the observation group and the control group. The associated responses of inflammation factors related to myocardial infarction were determined by molecular analysis. The levels of factors in patients with AMI were significantly higher than those in healthy subjects (P < 0.05). These findings suggest that a nano gold strip can be used for diagnosing early myocardial infarction.

scholarly journals The Spotted Contact Binary SS Arietis — Spectroscopy and Infrared Photometry

1992 ◽  
Vol 151 ◽  
pp. 379-382
Author(s):  
P.P. Rainger ◽  
S.A. Bell ◽  
R.W. Hilditch
Keyword(s):  
Imaging Techniques ◽  
Type System ◽  
Light Curves ◽  
Doppler Imaging ◽  
Mass Ratio ◽  
Infrared Photometry ◽  
High Quality ◽  
Precise Location ◽  
Medium Resolution ◽  
Contact Binary

The first infrared photometry for the W-UMa system SS Ari is presented. An analysis based on medium resolution spectroscopy presented here shows that SS Ari is a W-type system with a mass ratio of 0.33. It seems certain that the asymmetry in the published light curves and those obtained for this study can be explained by the effect of spots on one or possibly both components of the system. The precise location, size and temperature of these spots require the use of Doppler Imaging techniques in conjunction with high quality multi-band photometry.

scholarly journals Fluoropolymer Nanosheet as a Wrapping Mount for High‐Quality Tissue Imaging

2017 ◽  
Vol 29 (37) ◽  
pp. 1703139 ◽  
Author(s):  
Hong Zhang ◽  
Ami Masuda ◽  
Ryosuke Kawakami ◽  
Kenji Yarinome ◽  
Riku Saito ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
High Quality

scholarly journals Top Priorities for Cerebroprotective Studies: A Paradigm Shift

Stroke ◽  
2021 ◽  
Author(s):  
Patrick Lyden ◽  
Alastair Buchan ◽  
Johannes Boltze ◽  
Marc Fisher ◽  
Keyword(s):  
Ischemic Stroke ◽  
Brain Tissue ◽  
Imaging Techniques ◽  
Neurovascular Unit ◽  
Clinical Trial Design ◽  
Clinical Work ◽  
Brain Regions ◽  
Tissue Imaging ◽  
Health Concern ◽  
Stroke Treatment

Despite years of basic research and pioneering clinical work, ischemic stroke remains a major public health concern. Prior STAIR (Stroke Treatment Academic Industry Roundtable) conferences identified both failures of clinical trial design and failures in preclinical assessment in developing putative ischemic stroke treatments. At STAIR XI, participants in workshop no. 1 Top Priorities for Neuroprotection sought to redefine the neuroprotection paradigm and given the paucity of evidence underlying preclinical assessment, offer consensus-based recommendations. STAIR proposes the term brain cytoprotection or cerebroprotection to replace the term neuroprotection when the intention of an investigation is to demonstrate that a new, candidate treatment benefits the entire brain. Although “time is still brain,” tissue imaging techniques have been developed to identify patients with both predicted core injury and penumbral, salvageable brain tissue, regardless of time after stroke symptom onset. STAIR XI workshop participants called this imaging approach a tissue window to select patients for recanalization. Elements of the neurovascular unit show differential vulnerability evolving over differing time scales in different brain regions. STAIR proposes the term target window to suggest therapies that target the different elements of the neurovascular unit at different times. Based on contemporary principles of rigor and transparency, the workshop updated, revised, and enhanced the STAIR preclinical recommendations for developing new treatments in 2 phases: an exploratory qualification phase and a definitive validation phase. For new, putative treatments, investigators should carefully characterize the mechanism of action, the pharmacokinetics/pharmacodynamics, demonstrate target engagement, and confirm penetration through the blood-brain barrier. Before clinical trials, testing of candidate molecules in stroke models could proceed in a comprehensive manner using animals of both sexes and to include significant variables such as age and comorbid conditions. Comprehensive preclinical assessment might include multicenter, collaborative testing, for example, network trials. In the absence of a proven cerebroprotective agent to use as a gold standard, however, it remains speculative whether such comprehensive preclinical assessment can effectively predict clinical outcome.

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