In situ conversion of rose bengal microbubbles into nanoparticles for ultrasound imaging guided sonodynamic therapy with enhanced antitumor efficacy

2020 ◽  
Vol 8 (9) ◽  
pp. 2526-2536 ◽  
Author(s):  
Rui Hou ◽  
Xiaolong Liang ◽  
Xiaoda Li ◽  
Xu Zhang ◽  
Xiaotu Ma ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Rose Bengal ◽  
Drug Accumulation ◽  
Antitumor Efficacy ◽  
Sonodynamic Therapy

Sonosensitizer microbubbles enhance drug accumulation and the antitumor efficacy of sonodynamic therapy by ultrasound mediated micro to nano conversion.

pH/Ultrasound Dual-Responsive Gas Generator for Ultrasound Imaging-Guided Therapeutic Inertial Cavitation and Sonodynamic Therapy

2017 ◽  
Vol 7 (5) ◽  
pp. 1700957 ◽  
Author(s):  
Qianhua Feng ◽  
Wanxia Zhang ◽  
Xuemei Yang ◽  
Yuzhen Li ◽  
Yongwei Hao ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Gas Generator ◽  
Sonodynamic Therapy ◽  
Inertial Cavitation ◽  
Dual Responsive

Thermo‐Triggered In Situ Chitosan‐Based Gelation System for Repeated and Enhanced Sonodynamic Therapy Post a Single Injection

2020 ◽  
pp. 2001208
Author(s):  
Jialin She ◽  
Xuanfang Zhou ◽  
Yaojia Zhang ◽  
Rui Zhang ◽  
Quguang Li ◽  
...  
Keyword(s):  
Single Injection ◽  
Sonodynamic Therapy

scholarly journals Effect of cargo properties on in situ forming implant behavior determined by noninvasive ultrasound imaging

2012 ◽  
Vol 2 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Luis Solorio ◽  
Alexander M. Olear ◽  
Haoyan Zhou ◽  
Ashlei C. Beiswenger ◽  
Agata A. Exner
Keyword(s):  
Ultrasound Imaging ◽  
In Situ Forming

In situ ultrasound imaging of silk hydrogel degradation and neovascularization

2015 ◽  
Vol 11 (3) ◽  
pp. 822-830 ◽  
Author(s):  
Xiaoping Leng ◽  
Bin Liu ◽  
Bo Su ◽  
Min Liang ◽  
Liangchen Shi ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Silk Hydrogel

scholarly journals Novel epifluorescence microscopy method to determine life position of foraminifera in sediments

1996 ◽  
Vol 15 (1) ◽  
pp. 68-68 ◽  
Author(s):  
Joan M. Bernhard ◽  
Samuel S. Bowser
Keyword(s):  
Rose Bengal ◽  
Epifluorescence Microscopy ◽  
Fine Grained ◽  
Low Viscosity ◽  
Transmitted Light Microscopy ◽  
Embedded Cores ◽  
Microscopy Method ◽  
Novel Method ◽  
Vacuum Apparatus

Abstract. Microscopic examination of resin-embedded sedimenl cores has provided information regarding the sedimentary fabric in certain environments (e.g. Watling, 1988) as well as the in situ associations of rose Bengal-stained infaunal foraminifera (e.g. Frankel, 1970, 1974). Unfortunately, difficulties connected with making and examining these resin-embedded cores have limited their widespread use in studying meioinfauna. In particular, sediment grains may obscure specimens when viewed by transmitted light microscopy. Also, using rose Bengal to identify live foraminifera can be problematic (Bernhard, 1988). Furthermore, fine-grained deposits are difficult to infiltrate with highly viscous embedding media (e.g. Epon 812 ≥ 100 centipoise). and may require a vacuum apparatus for proper infiltration (Watling, 1988).Fluorescent probes were recently used to distinguish live from dead foraminifera (Bernhard et al., 1995). Here we report a novel method using an aldehyde-fixable fluorescent probe (Cell Tracker™ Green CMFDA) which, when viewed by epifluorescence microscopy, greatly enhances the visibility of biological material alive at the time of fixation. Cell Tracker™ Green CMFDA is a membrane-permeant chloromethyl derivative of fluorescein diacetate that is hydrolyzed by cellular esterases to yield a membrane-impermeant, fluorescent intermediate which further undergoes a reaction with glutathione to form an aldehyde-fixable end-product (Haugland, 1992). We also used low viscosity embedding media (i.e. 20–60 centipoise) to facilitate infiltration. The method is applied to the study of live foraminifera in a sediment core.PROCEDUREA c. 1.5 cm diameter subcorer (fashioned from a 10 ml syringe by removing the tip end, creating a cylinder into which two syringe plungers were inserted) was used to . . .

scholarly journals Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in situ and in vivo

1999 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
G J Czarnota ◽  
M C Kolios ◽  
J Abraham ◽  
M Portnoy ◽  
F P Ottensmeyer ◽  
...  
Keyword(s):  
Cell Death ◽  
High Resolution ◽  
Programmed Cell Death ◽  
Ultrasound Imaging ◽  
Invasive Monitoring ◽  
Non Invasive

scholarly journals Low Intensity Focused Ultrasound Augmented Multifunctional Nanoparticles for Integrating Ultrasound Imaging and Synergistic Therapy of Metastatic Breast Cancer

2021 ◽  
Author(s):  
Qian Zhang ◽  
Wen Wang ◽  
Hongyuan Shen ◽  
Hongyu Tao ◽  
Yating Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ultrasound Imaging ◽  
Focused Ultrasound ◽  
Metastatic Breast ◽  
Sonodynamic Therapy ◽  
Non Invasive ◽  
Low Intensity ◽  
Negative Effect

Abstract The metastasis of breast cancer is believed to have a negative effect on its prognosis. Benefiting from the remarkable deep-penetrating and non-invasive characteristics, sonodynamic therapy (SDT) demonstrates a whole series of potential leading to cancer treatment. To relieve the limitation of monotherapy, a multifunctional nanoplatform has been explored to realize the synergistic treatment efficiency. Herein, we establish a novel multifunctional nano-system which encapsulates chlorin e6 (Ce6, for SDT), perfluoropentane (PFP, for ultrasound imaging), and docetaxel (DTX, for chemotherapy) in a well-designed PLGA core-shell structure. The synergistic nanoparticle (CPDP NPs) featured with excellent biocompatibility and stability primarily enables its further application. Upon low intensity focused ultrasound (LIFU) irradiation, the enhanced ultrasound imaging could be revealed both in vitro and in vivo. More importantly, combined with LIFU, the nanoparticle exhibits intriguing antitumor capability through Ce6 induced cytotoxic reactive oxygen species as well as DTX releasing to generate a concerted therapeutic efficiency. Furthermore, this treating strategy actives a strong anti-metastasis capability by which lung metastatic nodules have been significantly reduced. The results indicate that the SDT-oriented nanoplatform combined with chemotherapy could be provided as a promising approach in elevating effective synergistic therapy and suppressing lung metastasis of breast cancer.

scholarly journals Contrast-enhanced sonography with biomimetic lung surfactant nanodrops

2020 ◽  
Author(s):  
Alec N. Thomas ◽  
Kang-Ho Song ◽  
Awaneesh Upadhyay ◽  
Virginie Papadopoulou ◽  
David Ramirez ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Lung Surfactant ◽  
Liquid Core ◽  
Acoustic Droplet Vaporization ◽  
Droplet Vaporization ◽  
Contrast Enhanced ◽  
Biomimetic Approach ◽  
The Stability

AbstractNanodrops comprising a perfluorocarbon liquid core can be acoustically vaporized into echogenic microbubbles for ultrasound imaging. Packaging the microbubble in its condensed liquid state provides distinct advantages, including in situ activation of the acoustic signal, longer circulation persistence, and the advent of expanded diagnostic and therapeutic applications in pathologies which exhibit compromised vasculature. One obstacle to clinical translation is the inability of the limited surfactant present on the nanodrop to encapsulate the greatly expanded microbubble interface, resulting in ephemeral microbubbles with limited utility. In this study, we examine a biomimetic approach to stabilizing an expanding gas surface by employing the lung surfactant replacement, Beractant. Lung surfactant contains a suite of lipids and surfactant proteins that provides efficient shuttling of material from bilayer folds to the monolayer surface. We therefore hypothesized that Beractant would improve stability of acoustically vaporized microbubbles. To test this hypothesis, we characterized Beractant surface dilation mechanics and revealed a novel biophysical phenomenon of rapid interfacial melting, spreading and re-solidification. We then harnessed this unique spreading capability to increase the stability and echogenicity of microbubbles produced after acoustic droplet vaporization for in vivo ultrasound imaging. Such biomimetic lung surfactant-stabilized nanodrops may be useful for applications in ultrasound imaging and therapy.Graphical Abstract

Color-Doppler ultrasound imaging of in situ human kidney stones in a hyperbaric chamber

2016 ◽  
Vol 140 (4) ◽  
pp. 2983-2984
Author(s):  
Julianna C. Simon ◽  
Barbrina Dunmire ◽  
Bryan W. Cunitz ◽  
Oleg A. Sapozhnikov ◽  
Jeffrey Thiel ◽  
...  
Keyword(s):  
Doppler Ultrasound ◽  
Ultrasound Imaging ◽  
Kidney Stones ◽  
Color Doppler ◽  
Human Kidney ◽  
Color Doppler Ultrasound ◽  
Hyperbaric Chamber ◽  
Doppler Ultrasound Imaging

scholarly journals In situ thermal ablation augments antitumor efficacy of adoptive T cell therapy

2019 ◽  
Vol 36 (sup1) ◽  
pp. 22-36 ◽  
Author(s):  
Fumito Ito ◽  
Trupti D. Vardam ◽  
Michelle M. Appenheimer ◽  
Kevin H. Eng ◽  
Sandra O. Gollnick ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Therapy ◽  
Thermal Ablation ◽  
Adoptive T Cell Therapy ◽  
Antitumor Efficacy ◽  
T Cell Therapy
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