A TRACER culture invasion assay to probe the impact of cancer associated fibroblasts on head and neck squamous cell carcinoma cell invasiveness

2020 ◽  
Vol 8 (11) ◽  
pp. 3078-3094
Author(s):  
Teresa Dean ◽  
Nancy T. Li ◽  
Jose L. Cadavid ◽  
Laurie Ailles ◽  
Alison P. McGuigan
Keyword(s):  
Cell Invasion ◽  
Carcinoma Cell ◽  
Cancer Associated Fibroblasts ◽  
Invasion Assay ◽  
Squamous Cell Carcinoma Cell ◽  
Matrix Density ◽  
Tumour Cell Invasion ◽  
Cell Invasiveness ◽  
The Impact

A novel 3D in vitro co-culture platform to explore the interactions between cancer-associated fibroblasts and tumour cells that drive increased tumour cell invasion and the impact of matrix density.

scholarly journals CTEN Induces Tumour Cell Invasion and Survival and Is Prognostic in Radiotherapy-Treated Head and Neck Cancer

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2963
Author(s):  
Jason C. Fleming ◽  
Jeongmin Woo ◽  
Karwan Moutasim ◽  
Christopher J. Hanley ◽  
Steven J. Frampton ◽  
...  
Keyword(s):  
Head And Neck ◽  
Cell Invasion ◽  
Tumour Cell ◽  
3D Culture ◽  
Response To Therapy ◽  
Antibody Array ◽  
Hpv Status ◽  
Tumour Cell Invasion

Head and neck squamous cell carcinoma (HNSCC) is a heterogenous disease treated with surgery and/or (chemo) radiotherapy, but up to 50% of patients with late-stage disease develop locoregional recurrence. Determining the mechanisms underpinning treatment resistance could identify new therapeutic targets and aid treatment selection. C-terminal tensin-like (CTEN) is a member of the tensin family, upregulated in several cancers, although its expression and function in HNSCC are unknown. We found that CTEN is commonly upregulated in HNSCC, particularly HPV−ve tumours. In vitro CTEN was upregulated in HPV−ve (n = 5) and HPV+ve (n = 2) HNSCC cell lines. Stable shRNA knockdown of CTEN in vivo significantly reduced tumour growth (SCC-25), and functional analyses in vitro showed that CTEN promoted tumour cell invasion, colony formation and growth in 3D-culture (SCC-25, Detroit 562). RNA sequencing of SCC-25 cells following CTEN siRNA knockdown identified 349 differentially expressed genes (logFC > 1, p < 0.05). Gene ontology analysis highlighted terms relating to cell locomotion and apoptosis, consistent with in vitro findings. A membrane-based antibody array confirmed that CTEN regulated multiple apoptosis-associated proteins, including HSP60 and cleaved caspase-3. Notably, in a mixed cohort of HPV+ve and HPV−ve HNSCC patients (n = 259), we found a significant, independent negative association of CTEN with prognosis, limited to those patients treated with (chemo)radiotherapy, not surgery, irrespective of human papillomavirus (HPV) status. These data show that CTEN is commonly upregulated in HNSCC and exerts several functional effects. Its potential role in modulating apoptotic response to therapy suggests utility as a predictive biomarker or radio-sensitising target.

A New In-Vitro Model to Study Tumour Cell Invasion

Metastasis ◽  
1980 ◽  
pp. 28-32 ◽  
Author(s):  
R. Tchao ◽  
A. B. Schleich ◽  
M. Frick ◽  
A. Mayer
Keyword(s):  
Cell Invasion ◽  
Tumour Cell ◽  
In Vitro Model ◽  
Tumour Cell Invasion ◽  
Vitro Model
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