scholarly journals Real time monitoring of biofilm formation on coated medical devices for the reduction and interception of bacterial infections

2020 ◽  
Vol 8 (5) ◽  
pp. 1464-1477 ◽  
Author(s):  
Yasin Kurmoo ◽  
Andrew L. Hook ◽  
Daniel Harvey ◽  
Jean-Frédéric Dubern ◽  
Paul Williams ◽  
...  
Keyword(s):  
Endotracheal Tube ◽  
Real Time ◽  
Optical Fibre ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Bacterial Attachment ◽  
Real Time Monitoring ◽  
Optical Fibre Sensors

Optical fibre sensors successfully detect and quantify bacterial attachment and biofilm formation on uncoated and coated endotracheal tube surfaces.

scholarly journals Real time monitoring of water level and temperature in storage fuel pools through optical fibre sensors

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
S. Rizzolo ◽  
J. Périsse ◽  
A. Boukenter ◽  
Y. Ouerdane ◽  
E. Marin ◽  
...  
Keyword(s):  
Water Level ◽  
Real Time ◽  
Optical Fibre ◽  
Real Time Monitoring ◽  
Optical Fibre Sensors

scholarly journals Understanding How Staphylococcal Autolysin Domains Interact With Polystyrene Surfaces

2021 ◽  
Vol 12 ◽  
Author(s):  
Radha P. Somarathne ◽  
Emily R. Chappell ◽  
Y. Randika Perera ◽  
Rahul Yadav ◽  
Joo Youn Park ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Limited Proteolysis ◽  
Cd Spectroscopy ◽  
Bacterial Attachment ◽  
Binding Interaction ◽  
Biofilm Growth ◽  
Source Of Infection ◽  
Structural Details

Biofilms, when formed on medical devices, can cause malfunctions and reduce the efficiency of these devices, thus complicating treatments and serving as a source of infection. The autolysin protein of Staphylococcus epidermidis contributes to its biofilm forming ability, especially on polystyrene surfaces. R2ab and amidase are autolysin protein domains thought to have high affinity to polystyrene surfaces, and they are involved in initial bacterial attachment in S. epidermidis biofilm formation. However, the structural details of R2ab and amidase binding to surfaces are poorly understood. In this study, we have investigated how R2ab and amidase influence biofilm formation on polystyrene surfaces. We have also studied how these proteins interact with polystyrene nanoparticles (PSNPs) using biophysical techniques. Pretreating polystyrene plates with R2ab and amidase domains inhibits biofilm growth relative to a control protein, indicating that these domains bind tightly to polystyrene surfaces and can block bacterial attachment. Correspondingly, we find that both domains interact strongly with anionic, carboxylate-functionalized as well as neutral, non-functionalized PSNPs, suggesting a similar binding interaction for nanoparticles and macroscopic surfaces. Both anionic and neutral PSNPs induce changes to the secondary structure of both R2ab and amidase as monitored by circular dichroism (CD) spectroscopy. These changes are very similar, though not identical, for both types of PSNPs, suggesting that carboxylate functionalization is only a small perturbation for R2ab and amidase binding. This structural change is also seen in limited proteolysis experiments, which exhibit substantial differences for both proteins when in the presence of carboxylate PSNPs. Overall, our results demonstrate that the R2ab and amidase domains strongly favor adsorption to polystyrene surfaces, and that surface adsorption destabilizes the secondary structure of these domains. Bacterial attachment to polystyrene surfaces during the initial phases of biofilm formation, therefore, may be mediated by aromatic residues, since these residues are known to drive adsorption to PSNPs. Together, these experiments can be used to develop new strategies for biofilm eradication, ensuring the proper long-lived functioning of medical devices.

scholarly journals Real-time monitoring of Pseudomonas aeruginosa biofilm formation on endotracheal tubes in vitro

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Eva Pericolini ◽  
Bruna Colombari ◽  
Gianmarco Ferretti ◽  
Ramona Iseppi ◽  
Andrea Ardizzoni ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Biofilm Formation ◽  
Real Time Monitoring ◽  
Endotracheal Tubes

scholarly journals An Optical Fibre-Based Sensor for Real-Time Monitoring of Clinical Linear Accelerator Radiotherapy Delivery

2016 ◽  
Vol 22 (3) ◽  
pp. 35-42 ◽  
Author(s):  
Sinead O'Keeffe ◽  
Wenhui Zhao ◽  
Weimin Sun ◽  
Daxin Zhang ◽  
Zhuang Qin ◽  
...  
Keyword(s):  
Real Time ◽  
Optical Fibre ◽  
Linear Accelerator ◽  
Real Time Monitoring

scholarly journals RNA III Inhibiting Peptide Inhibits In Vivo Biofilm Formation by Drug-Resistant Staphylococcus aureus

2003 ◽  
Vol 47 (6) ◽  
pp. 1979-1983 ◽  
Author(s):  
Andrea Giacometti ◽  
Oscar Cirioni ◽  
Yael Gov ◽  
Roberto Ghiselli ◽  
Maria Simona Del Prete ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Quorum Sensing ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Drug Resistant ◽  
Dacron Graft

ABSTRACT Staphylococcus aureus is a prevalent cause of bacterial infections associated with indwelling medical devices. RNA III inhibiting peptide (RIP) is known to inhibit S. aureus pathogenesis by disrupting quorum-sensing mechanisms. RIP was tested in the present study for its ability to inhibit S. aureus biofilm formation in a rat Dacron graft model. The activity of RIP was synergistic with those of antibiotics for the complete prevention of drug-resistant S. aureus infections.

scholarly journals Real‐time Monitoring of Staphylococcus aureus Biofilm Formation Under Flow Condition in Microfluidic Chambers

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Abdulaziz Al Mouslem ◽  
Eber Feliciano ◽  
Leonardo De La Fuente ◽  
Peter R. Panizzi
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Flow Condition ◽  
Biofilm Formation ◽  
Real Time Monitoring ◽  
Microfluidic Chambers

scholarly journals Bacterial Biofilm Growth on 3D-Printed Materials

2021 ◽  
Vol 12 ◽  
Author(s):  
Donald C. Hall ◽  
Phillip Palmer ◽  
Hai-Feng Ji ◽  
Garth D. Ehrlich ◽  
Jarosław E. Król
Keyword(s):  
Medical Devices ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Bacterial Attachment ◽  
Bacterial Biofilm ◽  
Chronic Infections ◽  
Biofilm Growth ◽  
Wide Range ◽  
3D Printed ◽  
Printed Materials

Recent advances in 3D printing have led to a rise in the use of 3D printed materials in prosthetics and external medical devices. These devices, while inexpensive, have not been adequately studied for their ability to resist biofouling and biofilm buildup. Bacterial biofilms are a major cause of biofouling in the medical field and, therefore, hospital-acquired, and medical device infections. These surface-attached bacteria are highly recalcitrant to conventional antimicrobial agents and result in chronic infections. During the COVID-19 pandemic, the U.S. Food and Drug Administration and medical officials have considered 3D printed medical devices as alternatives to conventional devices, due to manufacturing shortages. This abundant use of 3D printed devices in the medical fields warrants studies to assess the ability of different microorganisms to attach and colonize to such surfaces. In this study, we describe methods to determine bacterial biofouling and biofilm formation on 3D printed materials. We explored the biofilm-forming ability of multiple opportunistic pathogens commonly found on the human body including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus to colonize eight commonly used polylactic acid (PLA) polymers. Biofilm quantification, surface topography, digital optical microscopy, and 3D projections were employed to better understand the bacterial attachment to 3D printed surfaces. We found that biofilm formation depends on surface structure, hydrophobicity, and that there was a wide range of antimicrobial properties among the tested polymers. We compared our tested materials with commercially available antimicrobial PLA polymers.

Sign in / Sign up

Export Citation Format

Share Document