Facile fluorescence strategy for sensitive detection of microcystin-LR based on dsDNA-templated copper nanoclusters

2020 ◽  
Vol 12 (13) ◽  
pp. 1752-1758 ◽  
Author(s):  
Yanli Zhang ◽  
Yanqiong Lai ◽  
Xia Teng ◽  
Shaofu Pu ◽  
Zhi Yang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Low Cost ◽  
Sensitive Detection ◽  
Label Free ◽  
Copper Nanoclusters ◽  
Double Strand Dna

We developed a simple, sensitive, low-cost and label-free method to detect microcystin-leucine-arginine (MC-LR) using double-strand DNA-templated copper nanoclusters (dsDNA-CuNCs) as fluorescent probes.

Double stranded DNA-templated copper nanoclusters as a novel fluorescent probe for label-free detection of rutin

2019 ◽  
Vol 11 (28) ◽  
pp. 3584-3589 ◽  
Author(s):  
Yanqiong Lai ◽  
Xia Teng ◽  
Yanli Zhang ◽  
Hongbin Wang ◽  
Pengfei Pang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Low Cost ◽  
Label Free ◽  
Copper Nanoclusters ◽  
Double Stranded Dna ◽  
Label Free Detection

In this study, we developed a simple, sensitive, low-cost and label-free method to detect rutin by using double-stranded DNA-templated copper nanoclusters (dsDNA-CuNCs) as a fluorescent probe.

Nano-fluorescent probes based on DNA-templated copper nanoclusters for fast sensing of thiocyanate

2020 ◽  
Vol 44 (40) ◽  
pp. 17296-17301
Author(s):  
Tao Lei ◽  
Ting Huang ◽  
Tianze Wang ◽  
Peng Yu ◽  
Taiping Qing ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Fluorescent Sensor ◽  
Label Free ◽  
Copper Nanoclusters

A fast and label-free fluorescent sensor was developed to determine SCN−via inhibiting the formation of DNA-templated copper nanoclusters (CuNCs).

Simple and sensitive detection of uracil–DNA glycosylase activity using dsDNA-templated copper nanoclusters as fluorescent probes

2016 ◽  
Vol 8 (22) ◽  
pp. 4319-4323 ◽  
Author(s):  
Manman Cao ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Fluorescent Probes ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Copper Nanoclusters ◽  
Uracil Dna Glycosylase

DNA-templated copper nanoclusters were used as a fluorescent nano-dye for the uracil–DNA glycosylase (UDG) assay. The proposed method is simple and sensitive.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

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