Immunosensing of prostate cancer in human plasma samples using immobilization of antibody on the surface of mesoporous silica-modified silver nanoparticles and its immunocomplex with prostate-specific antigen

2019 ◽  
Vol 11 (48) ◽  
pp. 6159-6167 ◽  
Author(s):  
Fatemeh Farshchi ◽  
Mohammad Hasanzadeh ◽  
Elham Solhi
Keyword(s):  
Prostate Cancer ◽  
Silver Nanoparticles ◽  
Mesoporous Silica ◽  
Human Plasma ◽  
Prostate Specific Antigen ◽  
Early Stage ◽  
Screening Method ◽  
Specific Antigen ◽  
Plasma Samples ◽  
Human Plasma Samples

An innovative screening method for the early stage diagnosis of prostate cancer in human plasma samples is proposed in this study.

An Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for the biosensing of the prostate-specific antigen in unprocessed human plasma samples

2019 ◽  
Vol 11 (44) ◽  
pp. 5661-5672 ◽  
Author(s):  
Mina Shaban ◽  
Mohammad Hasanzadeh ◽  
Elham Solhi
Keyword(s):  
Human Plasma ◽  
Prostate Specific Antigen ◽  
Signal Amplification ◽  
Specific Antigen ◽  
Plasma Samples ◽  
Real Samples ◽  
Human Plasma Samples ◽  
Sandwich Type ◽  
Amplification Strategy

A novel sandwich-type biosensor based on a signal amplification strategy of various nanocomposites was developed to monitor the prostate-specific antigen (PSA) in real samples.

An Innovative Immunoanalysis Strategy towards Sensitive Recognition of PSA Biomarker in Human Plasma Samples Using Flexible and Portable Paper Based Biosensor: A New Platform towards POC Detection of Cancer Biomarkers Using Integration of Pen-on Paper Technology with Immunoassays Methods

2021 ◽  
Vol 1 (1) ◽  
pp. 6-6
Author(s):  
Fatemeh Farshchi ◽  
Arezoo Saadati ◽  
Houman Kholafazad kordasht ◽  
Mohammad Hasanzadeh
Keyword(s):  
Prostate Cancer ◽  
Human Plasma ◽  
Specific Antigen ◽  
Cost Effective ◽  
Plasma Samples ◽  
Label Free ◽  
Free Paper ◽  
Detection Range ◽  
Efficient Treatment ◽  
Human Plasma Samples

Background: The prostate-specific antigen (PSA) is one of the best markers for detecting prostate cancer. Rapid and real time recognition of PSA biomarker could be helpful in the early diagnosis and efficient treatment of prostate cancer. One of the usual methods to identify this biomarker is ELISA, which has a picomolar detection range but requires specialized personnel and also this technique is time-consuming and expensive. Methods: Cost-effective POC devices are great solutions because they are very cost-effective, sensitive and simple, do not require expert operators and have a high response time in a short time. With that in mind, in this work, a novel and simple label-free paper-based electrochemical immunosensor were designed by using conductive Ag-ink and designed directly by pen on paper technique on the surface of photographic paper, which is a suitable substrate for antibody immobilization, for rapid detection of PSA. Results: Based on the obtained results, under the optimum conditions, the synthesized Ag ink has a great substrate for antibody (Ab) and antigen (Ag) immobilization. The linear range was from 0.001 to 30 μg/L and the obtained low limite of quantification (LLOQ) was 0.001μg/L. This immunosensor also tested in human plasma samples, which had good analytical power. Conclusion: The proposed paper-based immunoassay could be a hopefully new and cheap tool for the diagnosis of other biomarkers.

Prostate Cancer: Biology, Incidence, Detection Methods, Treatment Methods, and Vaccines

2020 ◽  
Vol 20 (10) ◽  
pp. 847-854
Author(s):  
Ronald Bartzatt
Keyword(s):  
Prostate Cancer ◽  
Cancer Treatment ◽  
Prostate Specific Antigen ◽  
Cancer Biology ◽  
Early Stage ◽  
Specific Antigen ◽  
Detection Methods ◽  
Hormone Refractory Prostate Cancer ◽  
High Risk Prostate Cancer ◽  
Hormone Refractory

Cancer of the prostate are cancers in which most incidences are slow-growing, and in the U.S., a record of 1.2 million new cases of prostate cancer occurred in 2018. The rates of this type of cancer have been increasing in developing nations. The risk factors for prostate cancer include age, family history, and obesity. It is believed that the rate of prostate cancer is correlated with the Western diet. Various advances in methods of radiotherapy have contributed to lowering morbidity. Therapy for hormone- refractory prostate cancer is making progress, for almost all men with metastases will proceed to hormone-refractory prostate cancer. Smoking cigarettes along with the presence of prostate cancer has been shown to cause a higher risk of mortality in prostate cancer. The serious outcome of incontinence and erectile dysfunction result from the cancer treatment of surgery and radiation, particularly for prostate- specific antigen detected cancers that will not cause morbidity or mortality. Families of patients, as well as patients, are profoundly affected following the diagnosis of prostate cancer. Poor communication between spouses during prostate cancer increases the risk for poor adjustment to prostate cancer. The use of serum prostate-specific antigen to screen for prostate cancer has led to a greater detection, in its early stage, of this cancer. Prostate cancer is the most common malignancy in American men, accounting for more than 29% of all diagnosed cancers and about 13% of all cancer deaths. A shortened course of hormonal therapy with docetaxel following radical prostatectomy (or radiation therapy) for high-risk prostate cancer has been shown to be both safe and feasible. Patients treated with docetaxel-estramustine had a prostate-specific antigen response decline of at least 50%. Cancer vaccines are an immune-based cancer treatment that may provide the promise of a non-toxic but efficacious therapeutic alternative for cancer patients. Further studies will elucidate improved methods of detection and treatment.

Ultrasensitive photoelectrochemical immunoassay for prostate-specific antigen based on silver nanoparticles-triggered ion-exchange reaction with ZnO/CdS nanorods

The Analyst ◽  
2021 ◽  
Author(s):  
Ling Zhu ◽  
Zipeng Yin ◽  
Zijian Lv ◽  
Mei-Jin Li ◽  
Dianping Tang
Keyword(s):  
Prostate Cancer ◽  
Silver Nanoparticles ◽  
Ion Exchange ◽  
Exchange Reaction ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Cds Nanorods ◽  
Ion Exchange Reaction ◽  
Photoelectrochemical Immunoassay

Prostate-specific antigen (PSA), a glycoprotein that is most likely to cause prostate cancer, has attracted widespread attention in recent years due to its increasing threat to people's life and health....

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