A new HPLC method for simultaneous analysis of sterols, tocopherols, tocotrienols, and squalene in olive oil deodorizer distillates using a monolithic column with chemometric techniques

2019 ◽  
Vol 11 (36) ◽  
pp. 4681-4692 ◽  
Author(s):  
İsmail Tarhan ◽  
Hüseyin Kara
Keyword(s):  
Olive Oil ◽  
Simultaneous Determination ◽  
Monolithic Column ◽  
Hplc Method ◽  
Simultaneous Analysis ◽  
Deodorizer Distillate ◽  
Chemometric Techniques

Simultaneous determination: sterols, tocopherols, tocotrienols, and squalene in an olive oil deodorizer distillate have been separated using a monolithic column.

Development and validation of a new RP-HPLC method for the simultaneous determination of hydroquinone, kojic acid, octinoxate, avobenzone, BHA and BHT in skin-whitening cream

2016 ◽  
Vol 8 (5) ◽  
pp. 1170-1180 ◽  
Author(s):  
Fanny Galimany-Rovira ◽  
Pilar Pérez-Lozano ◽  
Josep Ma Suñé-Negre ◽  
Encarna García-Montoya ◽  
Montserrat Miñarro ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Kojic Acid ◽  
Cost Effective ◽  
Hplc Method ◽  
Simultaneous Analysis ◽  
Skin Whitening ◽  
Rp Hplc ◽  
Development And Validation

Simultaneous analysis with a single HPLC run allows for a much more cost-effective and less time-consuming analysis of skin-whitening cream.

scholarly journals HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets

2014 ◽  
Vol 64 (2) ◽  
pp. 187-198 ◽  
Author(s):  
Amer M. Alanazi ◽  
Ali S. Abdelhameed ◽  
Nasr Y. Khalil ◽  
Azmat A. Khan ◽  
Ibrahim A. Darwish
Keyword(s):  
Simultaneous Determination ◽  
High Throughput ◽  
Monolithic Column ◽  
Simple Procedure ◽  
Hplc Method ◽  
Uv Detector ◽  
Pharmaceutical Dosage Forms ◽  
Pharmaceutical Tablets ◽  
Short Run

Abstract A simple, sensitive and accurate HPLC method with high throughput has been developed and validated for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in combined pharmaceutical dosage forms. The proposed method employed, for the first time, a monolithic column in the analysis. Optimal chromatographic separation of the analytes was achieved on Chromolith® Performance RP-18e column using a mobile phase consisting of phosphate buffer (pH 4)/acetonitrile (50:50, V/V) pumped isocratically at a flow rate of 1.0 mL min-1. The eluted analytes were monitored with a UV detector set at 270 nm. Under the optimum chromatographic conditions, linear relationship with a good correlation coefficient (R ≥ 0.9997) was found between the peak area and the corresponding concentrations of both IRB and HCT in the ranges of 10-200 and 1-20 ng mL-1. The limits of detection were 2.34 and 0.03 ng mL-1 for IRB and HCT, respectively. The intra- and inter-assay precisions were satisfactory as the RSD values did not exceed 3 %. The accuracy of the proposed method was > 97 %. The proposed method had high throughput as the analysis involved a simple procedure and a very short run- -time of < 3 min. The results demonstrated that the method is applicable in the quality control of combined pharmaceutical tablets containing IRB and HCT

RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

2012 ◽  
Vol 2 (2) ◽  
pp. 364-367 ◽  
Author(s):  
Saida Naik Dheeravath ◽  
◽  
Kasani Ramadevi ◽  
Zilla Saraswathi ◽  
Dheeravath Maniklal ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Hplc Method ◽  
Rp Hplc ◽  
Hplc Method Development

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

A HPLC method for the simultaneous determination of seven anthracyclines

2005 ◽  
Vol 39 (1-2) ◽  
pp. 305-309 ◽  
Author(s):  
Irinel Badea ◽  
Lelia Lazăr ◽  
Dana Moja ◽  
Daniela Nicolescu ◽  
Aurel Tudose
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method
Sign in / Sign up

Export Citation Format

Share Document