A novel highly selective fluorescent probe for imaging of cysteine both in living cells and zebrafish

2019 ◽  
Vol 11 (34) ◽  
pp. 4323-4327 ◽  
Author(s):  
Li Wang ◽  
Mingguang Ren ◽  
Zihong Li ◽  
Lixuan Dai ◽  
Weiying Lin

We have developed a novel fluorescent probe (PI-Cys) for imaging cysteine in living cells and zebrafish. Fluorescence imaging shows that it can be used as an endogenous Cys probe and can even detect cysteine in zebrafish.

2019 ◽  
Vol 11 (19) ◽  
pp. 2565-2570 ◽  
Author(s):  
Zhao Cheng ◽  
Lei Zheng ◽  
Hao Xu ◽  
Long Pang ◽  
Hao He

A rhodamine-based fluorescent probe J1 with low cytotoxicity and characteristic fluorescence imaging of intracellular Fe3+ in living cells L929.


2015 ◽  
Vol 39 (11) ◽  
pp. 8940-8947 ◽  
Author(s):  
Sima Paul ◽  
Shyamaprosad Goswami ◽  
Chitrangada Das Mukhopadhyay

A ratiometric fluorescent probe having a fast response and a large Stokes shift detects SH− both in solid and vapour phases and this probe is used for fluorescence imaging of SH− in living cells.


2012 ◽  
Vol 48 (39) ◽  
pp. 4740 ◽  
Author(s):  
Keiichi Ohtsuka ◽  
Shinobu Sato ◽  
Yusuke Sato ◽  
Kojiro Sota ◽  
Shinsuke Ohzawa ◽  
...  

2019 ◽  
Vol 43 (2) ◽  
pp. 552-555 ◽  
Author(s):  
Li Wang ◽  
Mingguang Ren ◽  
Zihong Li ◽  
Lixuan Dai ◽  
Weiying Lin

We have developed a new small-molecule based, mitochondrial-targeted ratiometric fluorescent palladium(ii) probe (CR-Pd). Fluorescence imaging shows that CR-Pd is suitable for the ratiometric visualization of palladium(ii) in living cells.


2013 ◽  
Vol 23 (10) ◽  
pp. 2916-2919 ◽  
Author(s):  
Mao-Zhong Tian ◽  
Ming-Ming Hu ◽  
Jiang-Li Fan ◽  
Xiao-Jun Peng ◽  
Jing-Yun Wang ◽  
...  

2017 ◽  
Vol 5 (10) ◽  
pp. 1954-1961 ◽  
Author(s):  
Mingguang Ren ◽  
Beibei Deng ◽  
Kai Zhou ◽  
Jian-Yong Wang ◽  
Xiuqi Kong ◽  
...  

We have developed a new mitochondrial-targeted turn-on fluorescent HNO probe (Mito-HNO). Fluorescence imaging shows that Mito-HNO is suitable for ratiometric visualization of HNO within mitochondria in living cells.


2020 ◽  
Vol 56 (20) ◽  
pp. 3023-3026
Author(s):  
Shohei Uchinomiya ◽  
Naoya Matsunaga ◽  
Koichiro Kamoda ◽  
Ryosuke Kawagoe ◽  
Akito Tsuruta ◽  
...  

Fluorescence imaging of fatty acid beta oxidation (FAO) with a fluorescent probe metabolically degraded by sequential enzyme reactions of FAO.


2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Zhihao Guo ◽  
Xiuji Wang ◽  
Pei Wei ◽  
Yihua Gao ◽  
Qin Li

The development of fluorescent methods for the detection of metal ions is of great importance due to their diverse environmental and biological roles. Herein, a rhodamine 6G-based off-on fluorescent probe (L1) with a t-butyl pyrrole moiety as the recognition site was designed and synthesized. Photophysical studies show that L1 exhibits excellent sensitivity and selectivity towards Cu2+ to other metal ions in neutral acetonitrile aqueous media. Mechanism studies suggest that the recognition process may associate with a Cu2+ promoted hydrolysis reaction of L1. Furthermore, L1 has been successfully applied in fluorescence imaging of Cu2+ ion in living cells.


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