Isothermal cross-boosting extension–nicking reaction mediated exponential signal amplification for ultrasensitive detection of polynucleotide kinase

The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3742-3748 ◽  
Author(s):  
Xiao-Yu Li ◽  
Yun-Xi Cui ◽  
Yi-Chen Du ◽  
An-Na Tang ◽  
De-Ming Kong
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A novel nucleic acid-based isothermal signal amplification strategy, named cross-boosting extension–nicking reaction (CBENR) is developed and successfully used for rapid and ultrasensitive detection of polynucleotide kinase (PNK) activity.

A novel electrochemical biosensor for ultrasensitive Hg2+ detection via a triple signal amplification strategy

2021 ◽  
Author(s):  
Wang He ◽  
Bin Qiao ◽  
Fengzhen Li ◽  
Lisha Pan ◽  
Delun Chen ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Amplification Strategy

We report a novel electrochemical biosensor for the ultrasensitive Hg2+ detection via a signal amplification strategy. This strategy could be easily modified and extended to detect other hazardous heavy metals and nucleic acid.

scholarly journals Target-activated transcription for the amplified sensing of protease biomarkers

2020 ◽  
Vol 11 (11) ◽  
pp. 2993-2998 ◽  
Author(s):  
Fang Liu ◽  
Min Yang ◽  
Wenlu Song ◽  
Xingyu Luo ◽  
Rui Tang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Amplification Strategy

A transcription-inspired signal amplification strategy that amplifies proteolysis into nucleic acid outputs is presented for the ultrasensitive sensing of protease biomarkers.

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

Recent advance in exonuclease Ⅲ-assisted target signal amplification strategy for nucleic acid detection

2021 ◽  
Author(s):  
hongyu liu ◽  
Yuhao You ◽  
Youzhuo Zhu ◽  
Heng Zheng
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Diagnostics ◽  
Mutation Analysis ◽  
Signal Amplification ◽  
Nucleic Acid Detection ◽  
Target Signal ◽  
Forensic Investigations ◽  
Exo Iii ◽  
Amplification Strategy

Detection of nucleic acids have become significantly important in molecular diagnostics, genetics therapy, mutation analysis, forensic investigations and biomedical development, and so on. In recent years, exonuclease Ⅲ (Exo III)...

Hairpin DNA-fueled dynamic self-assembly of three-arm DNA branched junctions consisting of active DNAzyme structures for enzyme-free ultrasensitive detection of nucleic acids

2016 ◽  
Vol 8 (47) ◽  
pp. 8262-8265 ◽  
Author(s):  
Ke Yang ◽  
Ming Zeng ◽  
Xing He ◽  
Jianming Li ◽  
Dinggeng He
Keyword(s):  
Nucleic Acids ◽  
Self Assembly ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Hairpin Dna ◽  
Amplification Strategy ◽  
Dual Signal Amplification

An enzyme-free dual signal amplification strategy based on programmable molecular hairpins has been developed for amplified detection of DNA via the hairpin DNA-fueled dynamic self-assembly of three-arm DNAzyme.

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