scholarly journals Accuracy and precision analysis for a biophotonic assay of C-reactive protein

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2751-2757 ◽  
Author(s):  
Philip James-Pemberton ◽  
Urszula Łapińska ◽  
Mark Helliwell ◽  
Rouslan V. Olkhov ◽  
Oliver J. Hedaux ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
C Reactive Protein ◽  
Precision Analysis ◽  
Reactive Protein ◽  
Accuracy And Precision ◽  
Particle Plasmon

A multiplexed biophotonic assay platform has been developed using the localised particle plasmon in gold nanoparticles assembled in an array and functionalised for two assays: total IgG and C-reactive protein (CRP).

ANTIBODY CONJUGATES WITH GOLD NANOPARTICLES AS A COMPONENT OF IMMUNOCHROMATOGRAPHIC DIAGNOSTICUMS: THE INFLUENCE OF SYNTHESIS CONDITIONS ON THE COMPOSITION AND REACTIVITY

2020 ◽  
pp. 180-181
Author(s):  
N. Byzova ◽  
A. Zherdev ◽  
B. Dzantiev
Keyword(s):  
Gold Nanoparticles ◽  
Immunochromatographic Test ◽  
C Reactive Protein ◽  
Synthesis Conditions ◽  
Reactive Protein ◽  
Test Systems ◽  
Antibody Conjugates ◽  
Analytical Reagents ◽  
D Dimer

A series of preparations of gold nanoparticles with diameters from 13 to 60 nm and their conjugates with antibodies (murine immunoglobulins of class G) of different composition were obtained. The composition of the conjugates and the amount of antibodies that retain their reactivity in an immobilized form are characterized. Using the example of immunochromatographic test systems for the detection of D-dimer and C-reactive protein, the effectiveness of conjugates as analytical reagents is compared.

scholarly journals An aptasensor based on the microscopic enumeration of encoding gold nanoparticles for the detection of C-reactive protein

RSC Advances ◽  
2019 ◽  
Vol 9 (59) ◽  
pp. 34293-34298 ◽  
Author(s):  
Yuanfang Zhao ◽  
Jingru Zhao ◽  
Tian Jin ◽  
Shuqing Sun ◽  
Wenlan Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Magnetic Separation ◽  
C Reactive Protein ◽  
Reactive Protein

An aptasensor based on the displacement of encoding AuNPs by analyte molecules was presented. Combined with magnetic separation and DFM imaging, the number of displaced AuNPs was counted, which was correlated to the concentration of the CRP.

A simple aptamer-based colorimetric assay for rapid detection of C-reactive protein using gold nanoparticles

Talanta ◽  
2020 ◽  
Vol 214 ◽  
pp. 120868 ◽  
Author(s):  
Maria António ◽  
Rita Ferreira ◽  
Rui Vitorino ◽  
Ana L. Daniel-da-Silva
Keyword(s):  
Gold Nanoparticles ◽  
Rapid Detection ◽  
Colorimetric Assay ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals CRP Detection Using Gold Nanosensors – Effect of Layer Thickness and Immobilization Chemistry

2021 ◽  
Author(s):  
Stephan Kastner ◽  
Pia Pritzke ◽  
Andrea Csáki ◽  
Wolfgang Fritzsche
Keyword(s):  
Gold Nanoparticles ◽  
Gold Surface ◽  
Localized Surface Plasmon ◽  
C Reactive Protein ◽  
Target Signal ◽  
Reactive Protein ◽  
Varying Thickness ◽  
Binding Event ◽  
Target Binding ◽  
Different Thickness

Abstract The immobilization of a capture molecule represents a crucial step for effective usage of gold nanoparticles in localized surface plasmon resonance (LSPR)-based bioanalytics. Depending on the immobilization method used, the resulting capture layer is of varying thickness. Thus, the target binding event takes place in different distances to the gold surface. Using the example of a C-reactive protein (CRP) immunoassay, different immobilization methods were tested and investigated with regard to their resulting target signal strength. The dependency of the target signal on the distance to the gold surface was investigated utilizing polyelectrolyte bilayers of different thickness. It could be experimentally demonstrated how much the LSPR-shift triggered by a binding event on the gold nanoparticles decreases with increasing distance to the gold surface. Thus, the sensitivity of an LSPR assay is influenced by the choice of immobilization chemistry.

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