scholarly journals Quantification of protein glycation using vibrational spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (10) ◽  
pp. 3686-3696
Author(s):  
Bethan S. McAvan ◽  
Aidan P. France ◽  
Bruno Bellina ◽  
Perdita E. Barran ◽  
Royston Goodacre ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Protein Glycation ◽  
Glycated Proteins

FTIR-ATR and Raman spectroscopy can distinguish between glycated and non-glycated proteins.

scholarly journals Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 922
Author(s):  
William Querido ◽  
Shital Kandel ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Biological Tissues ◽  
Label Free ◽  
Connective Tissues ◽  
Bone Properties ◽  
Composition And Structure

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

scholarly journals Identification of LDPE Grades Focusing on Specific CH2 Raman Vibration Modes

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Richard Jumeau ◽  
Patrice Bourson ◽  
Michel Ferriol ◽  
François Lahure ◽  
Marc Ponçot ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Phenomenological Model ◽  
Low Density ◽  
Vibration Modes ◽  
Scanning Calorimetry ◽  
Lamella Thickness ◽  
Poly Ethylene

The possibilities of applications of vibrational spectroscopy techniques (Raman spectroscopy) in the analysis and characterization of polymers are more and more used and accurate. In this paper, our purpose is to characterize Low Density Poly(Ethylene) (LDPE) grades by Raman spectroscopy and in particular with CH2 Raman vibration modes. With temperature measurements, we determine different amorphous and crystalline Raman assignments. From these results and on the basis of the evolution of CH2 bending Raman vibration modes, we develop a phenomenological model in correlation with Differential Scanning Calorimetry and in particular with crystalline lamella thickness determination.

Infrared and Raman spectroscopy of automotive paints for forensic identification of natural weathering

2018 ◽  
Vol 10 (10) ◽  
pp. 1203-1212 ◽  
Author(s):  
Andrey Giovanni Gomes de Oliveira ◽  
Ewelina Wiercigroch ◽  
Juliano de Andrade Gomes ◽  
Kamilla Malek
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Forensic Identification ◽  
Natural Weathering ◽  
Infrared And Raman Spectroscopy ◽  
Full Analysis

A rapid and full analysis of automotive paints affected by natural weathering is proposed by means of vibrational spectroscopy.

Protein Glycation: An Old Villain is Shedding Secrets

2019 ◽  
Vol 22 (6) ◽  
pp. 362-369
Author(s):  
Gerald H. Lushington ◽  
Anthony C. Barnes
Keyword(s):  
Drug Targets ◽  
Current Knowledge ◽  
Treatment Options ◽  
Protein Glycation ◽  
Target Area ◽  
Disease Etiology ◽  
Glycated Proteins ◽  
Parkinson’S Diseases ◽  
Challenges And Opportunities ◽  
Novel Drug

: The glycation of proteins is non-physiological post-translational incorporation of carbohydrates onto the free amines or guanidines of proteins and some lipids. Although the existence of glycated proteins has been known for forty years, a full understanding of their pathogenic nature has been slow in accruing. In recent years, however, glycation has gained widespread acceptance as a contributing factor in numerous metabolic, autoimmune, and neurological disorders, tying together several confounding aspects of disease etiology. From diabetes, arthritis, and lupus, to multiple sclerosis, amyotrophic lateral sclerosis, Alzheimer’s, and Parkinson’s diseases, an emerging glycation/inflammation paradigm now offers significant new insight into a physiologically important toxicological phenomenon. It exposes novel drug targets and treatment options, and may even lay foundations for long-awaited breakthroughs. : This ‘current frontier’ article briefly profiles current knowledge regarding the underlying causes of glycation, the structural biology implications of such modifications, and their pathological consequences. Although several emerging therapeutic strategies for addressing glycation pathologies are introduced, the primary purpose of this mini-review is to raise awareness of the challenges and opportunities inherent in this emerging new medicinal target area.

scholarly journals Population-Controlled Impulsive Vibrational Spectroscopy: Background- and Baseline-Free Raman Spectroscopy of Excited Electronic States

2014 ◽  
Vol 118 (43) ◽  
pp. 9976-9984 ◽  
Author(s):  
Torsten Wende ◽  
Matz Liebel ◽  
Christoph Schnedermann ◽  
Robert J. Pethick ◽  
Philipp Kukura
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Electronic States ◽  
Excited Electronic States

Is serum fructosamine assay specific for determination of glycated serum protein?

1988 ◽  
Vol 34 (2) ◽  
pp. 320-323 ◽  
Author(s):  
E D Schleicher ◽  
R Mayer ◽  
E M Wagner ◽  
K D Gerbitz
Keyword(s):  
Healthy Subjects ◽  
Serum Proteins ◽  
Protein Glycation ◽  
Specific Product ◽  
Glycated Proteins ◽  
Liquid Chromatographic Method ◽  
Serum Fructosamine ◽  
Liquid Chromatographic ◽  
Hydrolysis Of

Abstract We compared the fructosamine activity in sera from healthy and diabetic subjects with the degree of protein glycation detected by a liquid-chromatographic method. The latter technique measures furosine as a specific product after hydrolysis of epsilon-amino-fructose-lysine. Our results indicate that the fructosamine assay measures the extent of glycation of purified human serum albumin correctly. On the other hand, we found no correlation between the two methods for sera from healthy subjects, although for diabetics' sera the values obtained with both methods were related. However, only about half of the reducing activity (fructosamine) was due to specific nonenzymatic glycation of proteins in healthy subjects and well-controlled diabetics. The remaining unspecific activity varied from serum to serum. It was not reducible with NaBH4 and was independent of the glycation of albumin, which normally accounts for about 80% of glycated serum proteins. The fructosamine assay is therefore of limited specificity for the exact measurement of glycated proteins in serum.

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