scholarly journals The search for a unique Raman signature of amyloid-beta plaques in human brain tissue from Alzheimer's disease patients

The Analyst ◽  
2020 ◽  
Vol 145 (5) ◽  
pp. 1724-1736 ◽  
Author(s):  
Benjamin Lochocki ◽  
Tjado H. J. Morrema ◽  
Freek Ariese ◽  
Jeroen J. M. Hoozemans ◽  
Johannes F. de Boer
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Raman Spectroscopy ◽  
Human Brain ◽  
Brain Tissue ◽  
Amyloid Beta ◽  
Spectral Signature ◽  
Human Brain Tissue ◽  
Formalin Fixed

Raman spectroscopy was used to examine unstained, formalin fixed Alzheimer's disease human brain tissue to potentially identify a unique spectral signature of amyloid-beta plaques.

scholarly journals Luminex-based quantification of Alzheimer’s disease neuropathologic change in formalin-fixed post-mortem human brain tissue

2018 ◽  
Vol 99 (7) ◽  
pp. 1056-1067
Author(s):  
C. Dirk Keene ◽  
Angela M. Wilson ◽  
Mitchell D. Kilgore ◽  
Lauren T. Bruner ◽  
Nadia O. Postupna ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Brain ◽  
Brain Tissue ◽  
Post Mortem ◽  
Human Brain Tissue ◽  
Formalin Fixed

scholarly journals Detection of NGF-like activity in human brain tissue: increased levels in Alzheimer's disease

1993 ◽  
Vol 13 (6) ◽  
pp. 2540-2550 ◽  
Author(s):  
KA Crutcher ◽  
SA Scott ◽  
S Liang ◽  
WV Everson ◽  
J Weingartner
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Brain ◽  
Brain Tissue ◽  
Human Brain Tissue

Identification of amyloid-beta (Aβ) plaques in freshly frozen human brain tissue using Raman spectroscopy

2019 ◽  
Author(s):  
Benjamin Lochocki ◽  
Tjado H. J. Morrema ◽  
Freek Ariese ◽  
Jeroen J. M. Hoozemans ◽  
Johannes F. de Boer
Keyword(s):  
Raman Spectroscopy ◽  
Human Brain ◽  
Brain Tissue ◽  
Amyloid Beta ◽  
Human Brain Tissue

scholarly journals Correlative x-ray phase-contrast tomography and histology of human brain tissue affected by Alzheimer’s disease

NeuroImage ◽  
2020 ◽  
Vol 210 ◽  
pp. 116523 ◽  
Author(s):  
Mareike Töpperwien ◽  
Franziska van der Meer ◽  
Christine Stadelmann ◽  
Tim Salditt
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Brain ◽  
Brain Tissue ◽  
Phase Contrast ◽  
Human Brain Tissue ◽  
X Ray ◽  
Phase Contrast Tomography

Organotin compounds in trimethyltin-treated rats and in human brain in Alzheimer's Disease

1997 ◽  
Vol 16 (9) ◽  
pp. 512-515 ◽  
Author(s):  
F. Martin ◽  
FM Corrigan ◽  
Ofx Donard ◽  
J. Kelly ◽  
Jao Besson ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Molecular Weight ◽  
Human Brain ◽  
Brain Tissue ◽  
Hydride Generation ◽  
Organotin Compounds ◽  
Low Molecular Weight ◽  
Human Brain Tissue ◽  
Cryogenic Trapping

As blood tin concentrations are elevated in Alzheimer's disease and as some low molecular weight organotin compounds are neurotoxic, we have attempted to detect organotins in brain in Alzheimer's Disease. First we measured the concentration of trimethyltin (TMT) in the brains of rats which had been exposed to memory- impairing concentrations of TMT and, as the method of linking hydride generation, cryogenic trapping, gas chromatographic separation and atomic absorption spec trophotometric detection permitted the measurements of organotin compounds when the total tin was greater than 0.2 nanograms, we applied these techniques to human brain tissue, some of which showed neuropathological evidence of Alzheimer's Disease. No low molecular weight organotin compounds were detected in the human brain tissue, but it is possible that tin may be complexed with large organic molecules, the hydrides of which would not be volatile, but which could be identified by liquid chromatography.

scholarly journals Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Benjamin Lochocki ◽  
Baayla D. C. Boon ◽  
Sander R. Verheul ◽  
Liron Zada ◽  
Jeroen J. M. Hoozemans ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Brain ◽  
Brain Tissue ◽  
Plaque Morphology ◽  
Label Free ◽  
Human Brain Tissue ◽  
Histological Techniques ◽  
Plaque Area ◽  
Amyloid Deposits

AbstractAlzheimer’s disease (AD) neuropathology is characterized by hyperphosphorylated tau containing neurofibrillary tangles and amyloid-beta (Aβ) plaques. Normally these hallmarks are studied by (immuno-) histological techniques requiring chemical pretreatment and indirect labelling. Label-free imaging enables one to visualize normal tissue and pathology in its native form. Therefore, these techniques could contribute to a better understanding of the disease. Here, we present a comprehensive study of high-resolution fluorescence imaging (before and after staining) and spectroscopic modalities (Raman mapping under pre-resonance conditions and stimulated Raman scattering (SRS)) of amyloid deposits in snap-frozen AD human brain tissue. We performed fluorescence and spectroscopic imaging and subsequent thioflavin-S staining of the same tissue slices to provide direct confirmation of plaque location and correlation of spectroscopic biomarkers with plaque morphology; differences were observed between cored and fibrillar plaques. The SRS results showed a protein peak shift towards the β-sheet structure in cored amyloid deposits. In the Raman maps recorded with 532 nm excitation we identified the presence of carotenoids as a unique marker to differentiate between a cored amyloid plaque area versus a non-plaque area without prior knowledge of their location. The observed presence of carotenoids suggests a distinct neuroinflammatory response to misfolded protein accumulations.

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