Ligand structure and charge state-dependent separation of monolayer protected Au25 clusters using non-aqueous reversed-phase HPLC

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1337-1345
Author(s):  
Korath Shivan Sugi ◽  
Shridevi Bhat ◽  
Abhijit Nag ◽  
Paramasivam Ganesan ◽  
Ananthu Mahendranath ◽  
...  
Keyword(s):  
Charge State ◽  
Flow Rate ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Ligand Structure ◽  
Rate Dependent ◽  
State Dependent

We demonstrate a systematic flow rate dependent study of three different aliphatic ligand protected Au25 clusters, with three commercially available reversed-phase HPLC columns.

A study of the separation of Mo(VI) and W(VI) by reversed phase HPLC

1989 ◽  
Vol 54 (8) ◽  
pp. 2133-2140 ◽  
Author(s):  
Milan Vrchlabský ◽  
Naděžda Pollaková ◽  
Aleš Hrdlička
Keyword(s):  
Flow Rate ◽  
Phosphate Buffer ◽  
Stream Flow ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation ◽  
Rp Hplc

The RP HPLC separation of Mo(VI) and W(VI) in the form of their oxoanions or anionic chelates with 2,3-dihydroxynaphthalene (DHN) was studied using a 100 x 4 mm i.d. column packed with Silasorb C18 (10μm). The two elements were separated with a resolution of Rij = 1.8 in 10 min after injection of the DHN-derivatized anions into the stream (flow rate 1 ml min-1) of the mobile phase containing phosphate buffer (50 mmol l-1), DHN (0.5 mmol l-1) and tetrabutylammonium cations (1 mmol l-1) in 60% (v/v) methanol at pH 7.

scholarly journals A Reversed-phase HPLC Study of the Complexation of Benzene Derivatives Guest Molecules with 5,17-bis(N-Tolyliminomethyl)-25,27-dipropoxycalix[4]arene in Acetonitrile–Water Solution

1999 ◽  
Vol 23 (1) ◽  
pp. 60-61
Author(s):  
O. I. Kalchenko ◽  
A. V. Solovyov ◽  
J. Lipkowski ◽  
V. I. Kalchenko
Keyword(s):  
Stability Constants ◽  
Water Solution ◽  
Reversed Phase ◽  
Benzene Derivatives ◽  
Reversed Phase Hplc ◽  
Guest Molecules ◽  
Hplc Study

Stability constants of the host–guest complexes of 5,17-bis( N-tolyliminomethyl)-25,27-dipropoxycalix[4]arene with benzene derivatives were determined by reversed-phase HPLC in acetonitrile–water solution.

scholarly journals Development and validation of RP-HPLC method for estimation of brexpiprazole in its bulk and tablet dosage form using Quality by Design approach

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Amol S. Jagdale ◽  
Nilesh S. Pendbhaje ◽  
Rupali V. Nirmal ◽  
Poonam M. Bachhav ◽  
Dayandeo B. Sumbre
Keyword(s):  
Flow Rate ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Control Analysis ◽  
Uv Detector ◽  
Mobile Phase Flow Rate ◽  
Rp Hplc ◽  
Quality By Design Approach ◽  
Bulk Drug

Abstract Background A new, sensitive, suitable, clear, accurate, and robust reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of brexpiprazole in bulk drug and tablet formulation was developed and validated in this research. Surface methodology was used to optimize the data, with a three-level Box-Behnken design. Methanol concentration in the mobile phase, flow rate, and pH were chosen as the three variables. The separation was performed using an HPLC method with a UV detector and Openlab EZchrom program, as well as a Water spherisorb C18 column (100 mm × 4.6; 5m). Acetonitrile was pumped at a flow rate of 1.0 mL/min with a 10 mM phosphate buffer balanced to a pH of 2.50.05 by diluted OPA (65:35% v/v) and detected at 216 nm. Result The developed RP-HPLC method yielded a suitable retention time for brexpiprazole of 4.22 min, which was optimized using the Design Expert-12 software. The linearity of the established method was verified with a correlation coefficient (r2) of 0.999 over the concentration range of 5.05–75.75 g/mL. For API and formulation, the percent assay was 99.46% and 100.91%, respectively. The percentage RSD for the method’s precision was found to be less than 2.0%. The percentage recoveries were discovered to be between 99.38 and 101.07%. 0.64 μg/mL and 1.95 μg/mL were found to be the LOD and LOQ, respectively. Conclusion The developed and validated RP-HPLC system takes less time and can be used in the industry for routine quality control/analysis of bulk drug and marketed brexpiprazole products. Graphical abstract

4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.

1977 ◽  
Vol 23 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
P H Culbreth ◽  
I W Duncan ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Nitrophenyl Phosphate ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.

Determination of 5-hydroxymethyl-2-furaldehyde in royal jelly by a rapid reversed phase HPLC method

2013 ◽  
Vol 5 (19) ◽  
pp. 5010 ◽  
Author(s):  
Marco Ciulu ◽  
Roberta Farre ◽  
Ignazio Floris ◽  
Valeria M. Nurchi ◽  
Angelo Panzanelli ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc

Simultaneous Determination of Ibuprofen and Pseudoephedrine Hydrochloride in Pharmaceutical Tablets by Reversed-Phase HPLC

2009 ◽  
Vol 42 (18) ◽  
pp. 2951-2961 ◽  
Author(s):  
Marie-Hélène Langlois ◽  
Philippe Dallet ◽  
Tina Kauss ◽  
Jean-Pierre Dubost
Keyword(s):  
Simultaneous Determination ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Pharmaceutical Tablets
Sign in / Sign up

Export Citation Format

Share Document