Parallel [TG(GA)3]n-homoduplexes/thioflavin T: an intense and stable fluorescent indicator for label-free biosensing

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 286-294 ◽  
Author(s):  
Qiang Liu ◽  
Shaochun Jing ◽  
Mei Liu ◽  
Yan Jin ◽  
Baoxin Li
Keyword(s):  
Thioflavin T ◽  
Label Free ◽  
Fluorescent Indicator ◽  
G Quadruplex

Compared with the G-quadruplex/ThT fluorescent system, parallel [TG(GA)3]n-dsDNA/ThT is a stable and strong fluorescent indicator for label-free biosensing.

scholarly journals A Sensitive and Label-Free Pb(II) Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex/Thioflavin T Conformation

Sensors ◽  
2016 ◽  
Vol 16 (12) ◽  
pp. 2155 ◽  
Author(s):  
Yanli Wen ◽  
Lele Wang ◽  
Lanying Li ◽  
Li Xu ◽  
Gang Liu
Keyword(s):  
Fluorescence Sensor ◽  
Thioflavin T ◽  
Label Free ◽  
G Quadruplex

A label-free fluorescence method for the detection of uracil DNA glycosylase activity based on G-quadruplex formation

2016 ◽  
Vol 8 (41) ◽  
pp. 7453-7459 ◽  
Author(s):  
Changbei Ma ◽  
Kefeng Wu ◽  
Jun Wang ◽  
Hailun He ◽  
Feng Ning ◽  
...  
Keyword(s):  
Fluorescence Method ◽  
Thioflavin T ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Quadruplex Formation

A simple fluorescence based biosensor for label-free detection of uracil DNA glycosylase activity based on G-quadruplex formation using a thioflavin T probe is reported.

scholarly journals Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe

Toxins ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 198 ◽  
Author(s):  
Kefeng Wu ◽  
Changbei Ma ◽  
Han Zhao ◽  
Hailun He ◽  
Hanchun Chen
Keyword(s):  
Ochratoxin A ◽  
Fluorescence Assay ◽  
Thioflavin T ◽  
Label Free ◽  
G Quadruplex

Label-free fluorometric detection of microRNA using isothermal rolling circle amplification generating tandem G-quadruplex

The Analyst ◽  
2020 ◽  
Vol 145 (18) ◽  
pp. 6130-6137
Author(s):  
Minhee Kim ◽  
Dong-Min Kim ◽  
Dong-Eun Kim
Keyword(s):  
Tandem Repeats ◽  
Rolling Circle Amplification ◽  
Thioflavin T ◽  
Fluorometric Detection ◽  
Label Free ◽  
Rolling Circle ◽  
G Quadruplex

Fluorometric detection of microRNA using Rolling Circle Amplification generating tandem G-quadruplex (GQ-RCA). Target miRNA triggers the GQ-RCA reaction generating tandem repeats of the G-quadruplex, resulting in enhanced Thioflavin T fluorescence.

scholarly journals A Label-Free Fluorometric Glutathione Assay Based on a Conformational Switch of G-quadruplex

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2743
Author(s):  
Xi Zhou ◽  
Doudou Zhang ◽  
Ying Yan ◽  
Hailun He ◽  
Yukui Zhou ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Clinical Diagnostics ◽  
Thioflavin T ◽  
Label Free ◽  
Serum Samples ◽  
Standard Samples ◽  
Conformational Switch ◽  
Fluorescent Intensity ◽  
G Quadruplex ◽  
Sensitivity And Selectivity

In this paper, a label-free fluorescent method for glutathione (GSH) detection based on a thioflavin T/G-quadruplex conformational switch is developed. The sensing assay is fabricated depending on the virtue of mercury ions to form a thymine–thymine mismatch, which collapses the distance between two ssDNA and directs the guanine-rich part to form an intra-strand asymmetric split G-quadruplex. The newly formed G-quadruplex efficiently reacts with thioflavin T and enhances the fluorescent intensity. In the presence of GSH, Hg2+ is absorbed, destroying the G-quadruplex formation with a significant decrease in fluorescence emission. The proposed fluorescent assay exhibits a linear range between 0.03–5 μM of GSH with a detection limit of 9.8 nM. Furthermore, the efficacy of this method is examined using human serum samples to detect GSH. Besides GSH, other amino acids are also investigated in standard samples, which display satisfactory sensitivity and selectivity. Above all, we develop a method with features including potentiality, facility, sensitivity, and selectivity for analyzing GSH for clinical diagnostics.

One-strand oligonucleotide probe for fluorescent label-free “turn-on” detection of T4 polynucleotide kinase activity and its inhibition

The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5650-5655 ◽  
Author(s):  
Fu Zhou ◽  
Guangfeng Wang ◽  
Dongmin Shi ◽  
Yue Sun ◽  
Liang Sha ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Kinase Activity ◽  
Oligonucleotide Probe ◽  
Thioflavin T ◽  
Fluorescent Label ◽  
Label Free ◽  
Molecular Rotor ◽  
Turn On ◽  
Polynucleotide Kinase ◽  
G Quadruplex

Thioflavin T (ThT), as one of the most exciting fluorogenic molecules, boasts the “molecular-rotor” ability to induce DNA sequences containing guanine repeats to fold into G-quadruplex structures.

Sign in / Sign up

Export Citation Format

Share Document