Facile one-step targeted immobilization of an enzyme based on silane emulsion self-assembled molecularly imprinted polymers for visual sensors

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 268-276 ◽  
Author(s):  
Guoning Chen ◽  
Hua Shu ◽  
Lu Wang ◽  
Kamran Bashir ◽  
Qun Wang ◽  
...  
Keyword(s):  
Crude Extract ◽  
Molecularly Imprinted Polymers ◽  
Self Assembly ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Step Process ◽  
Visual Sensors ◽  
One Step ◽  
Self Assembled

The silane emulsion self-assembly MIPs can specifically immobilize HRP in a one-step process from a crude extract of horseradish (unpurified samples).

Removal of disperse violet 28 from water using self-assembled organo-layered double hydroxides through a one-step process

RSC Advances ◽  
2014 ◽  
Vol 4 (102) ◽  
pp. 58307-58314 ◽  
Author(s):  
Yan Li ◽  
Hao-Yu Bi ◽  
Yong-Sheng Jin ◽  
Xiao-Qin Shi
Keyword(s):  
Layered Double Hydroxides ◽  
Self Assembly ◽  
The Self ◽  
Dyeing Wastewater ◽  
Step Process ◽  
One Step ◽  
Self Assembled ◽  
Double Hydroxides

A simple one-step process involving the self-assembly of organo-LDH and the removal of non-ionic dyes from dyeing wastewater was realized.

Synthesis and Characterization of Molecularly Imprinted Polymers for Quercetin

2013 ◽  
Vol 17 ◽  
pp. 71-78
Author(s):  
Neelam Verma ◽  
Nitu Trehan
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Fruits And Vegetables ◽  
Sem Analysis ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Inflammatory Agent ◽  
One Step ◽  
Ft Ir

Quercetin is a flavonoid present in herbs, fruits and vegetables. It acts as an antioxidant, anticancer and anti-inflammatory agent. Molecularly Imprinted Polymers (MIPs) were prepared for quercetin by non-covalent approach in the presence of monomer acrylamide, EGDMA as the crosslinker, AIBN as the initiator and methanol and ethyl acetate as porogenic solvents. Most stable MIPs were synthesized using methanol as the solvent. Step wise removal of the template with methanol and acetic acid (9:1) left binding sites that retain complementary specificity and affinity. These stable MIPs were analysed by FT-IR technique. It was observed that there was hydrogen bonding between the template and the functional monomer. This study was further supported by NMR analysis for MIPs and NMIPs (control polymer) that cavity for quercetin has been created in MIPs and absent in the case of NMIPs. MIPs were characterized by SEM analysis that showed more clusters in case of MIPs than NMIPs as an effect of imprinting. These MIPs can be used for extraction of quercetin from herbs in a one step process.

scholarly journals Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

Biosensors ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 16 ◽  
Author(s):  
Kai-Hsi Liu ◽  
Danny O’Hare ◽  
James L. Thomas ◽  
Han-Zhang Guo ◽  
Chien-Hsin Yang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Self Assembly ◽  
Conductive Polymers ◽  
Limit Of Detection ◽  
Electrochemical Determination ◽  
Aromatic Rings ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Target Molecules ◽  
Metanilic Acid

Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π-π and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted electronically conductive polymers (TIECPs) on sensing electrodes. The linear sensing range for testosterone was from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000-fold to measure testosterone concentration using the above TIECP sensors; results were compared with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33 ± 0.09 to 9.13 ± 1.33 ng/mL. The mean accuracy of the TIECP-coated sensors was 90.3 ± 7.0%.

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