scholarly journals Versatile printed microheaters to enable low-power thermal control in paper diagnostics

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 184-196
Author(s):  
Kristin M. Byers ◽  
Li-Kai Lin ◽  
Taylor J. Moehling ◽  
Lia Stanciu ◽  
Jacqueline C. Linnes
Keyword(s):  
Low Power ◽  
Point Of Care ◽  
Low Cost ◽  
Thermal Control ◽  
Point Of Care Diagnostics ◽  
Paper Diagnostics

Reusable, low-power, low-cost, printed microheaters developed for practical use in in-field and point-of-care diagnostics.

scholarly journals Pocket MUSE: an affordable, versatile and high-performance fluorescence microscope using a smartphone

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yehe Liu ◽  
Andrew M. Rollins ◽  
Richard M. Levenson ◽  
Farzad Fereidouni ◽  
Michael W. Jenkins
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
Consumer Electronics ◽  
Practical Implementation ◽  
The Novel ◽  
Point Of Care Diagnostics ◽  
Optical Module ◽  
Optical Configuration ◽  
User Friendly

AbstractSmartphone microscopes can be useful tools for a broad range of imaging applications. This manuscript demonstrates the first practical implementation of Microscopy with Ultraviolet Surface Excitation (MUSE) in a compact smartphone microscope called Pocket MUSE, resulting in a remarkably effective design. Fabricated with parts from consumer electronics that are readily available at low cost, the small optical module attaches directly over the rear lens in a smartphone. It enables high-quality multichannel fluorescence microscopy with submicron resolution over a 10× equivalent field of view. In addition to the novel optical configuration, Pocket MUSE is compatible with a series of simple, portable, and user-friendly sample preparation strategies that can be directly implemented for various microscopy applications for point-of-care diagnostics, at-home health monitoring, plant biology, STEM education, environmental studies, etc.

scholarly journals Corrigendum to “Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics” [Biosens. Bioelectron. 129 (2019) 147–154]

2020 ◽  
Vol 150 ◽  
pp. 111956
Author(s):  
Andrew Lakey ◽  
Zulfiqur Ali ◽  
Simon M. Scott ◽  
Syrine Chebil ◽  
Hafsa Korri-Youssoufi ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Point Of Care Diagnostics

scholarly journals The SARS-COV-2 Spike Protein Binds Sialic Acids, and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

2020 ◽  
Author(s):  
Alexander N. Baker ◽  
Sarah-Jane Richards ◽  
Collette S. Guy ◽  
Thomas R. Congdon ◽  
Muhammad Hasan ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Lateral Flow ◽  
Function Optimization ◽  
Spike Protein ◽  
Spike Glycoprotein ◽  
Binding Function ◽  
Point Of Care Diagnostics ◽  
Novel Coronavirus ◽  
Flow Detection

<div> <div> <div> <p>There is an urgent need to understand the behavior of novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has high affinity towards the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a viral particle mimic, paper-based lateral flow detection was demonstrated in under 30 minutes showing the potential of this system as a low-cost detection platform. </p> </div> </div> </div>

scholarly journals Handyfuge-LAMP: low-cost and electricity-free centrifugation for isothermal SARS-CoV-2 detection in saliva

2020 ◽  
Author(s):  
Ethan Li ◽  
Adam Larson ◽  
Anesta Kothari ◽  
Manu Prakash
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Sample Collection ◽  
Rna Detection ◽  
Open Hardware ◽  
Point Of Care Diagnostics ◽  
Simple Protocol ◽  
Specialized Equipment ◽  
The Cost

AbstractPoint of care diagnostics for COVID-19 detection are vital to assess infection quickly and at the source so appropriate measures can be taken. The loop-mediated isothermal amplification (LAMP) assay has proven to be a reliable and simple protocol that can detect small amounts of viral RNA in patient samples (<10 genomes per μL) (Nagamine, Hase, and Notomi 2002). Recently, Rabe and Cepko at Harvard published a sensitive and simple protocol for COVID-19 RNA detection in saliva using an optimized LAMP assay (Rabe and Cepko, 2020).This LAMP protocol has the benefits of being simple, requiring no specialized equipment; rapid, requiring less than an hour from sample collection to readout; and cheap, costing around $1 per reaction using commercial reagents. The pH based colorimetric readout also leaves little ambiguity and is intuitive. However, a shortfall in many nucleic acid-based methods for detection in saliva samples has been the variability in output due to the presence of inhibitory substances in saliva. Centrifugation to separate the reaction inhibitors from inactivated sample was shown to be an effective way to ensure reliable LAMP amplification. However, a centrifuge capable of safely achieving the necessary speeds of 2000 RPM for several minutes often costs hundreds of dollars and requires a power supply.We present here an open hardware solution- Handyfuge - that can be assembled with readily available components for the cost of <5 dollars a unit and could be used together with the LAMP assay for point of care detection of COVID-19 RNA from saliva. The device is then validated using the LAMP protocol from Rabe and Cepko. With the use of insulated coolers for reagent supply chain and delivery, the assay presented can be completed without the need for electricity or any laboratory scale infrastructure.

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