Responsive methylene blue release from lanthanide coordination polymer for label-free, immobilization-free and sensitive electrochemical alkaline phosphatase activity assay

The Analyst ◽  
2019 ◽  
Vol 144 (20) ◽  
pp. 5971-5979 ◽  
Author(s):  
Zhiqiang Chen ◽  
Shuang Liu ◽  
Xiaoxiao Yu ◽  
Lijie Hao ◽  
Li Wang ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Alkaline Phosphatase ◽  
Coordination Polymer ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Label Free ◽  
Activity Assay ◽  
Lanthanide Coordination Polymer ◽  
Stimulus Responsive

A stimulus responsive methylene blue releasing and enriching strategy for sensitive electrochemical alkaline phosphatase activity assay.

Fluorescence assay for alkaline phosphatase activity based on energy transfer from terbium to europium in lanthanide coordination polymer nanoparticles

2018 ◽  
Vol 6 (37) ◽  
pp. 6008-6015 ◽  
Author(s):  
Fengyi Wang ◽  
Xuan Hu ◽  
Jing Hu ◽  
Qianqian Peng ◽  
Baozhan Zheng ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Energy Transfer ◽  
Coordination Polymer ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Assay ◽  
Polymer Nanoparticles ◽  
Lanthanide Coordination Polymer

An effective fluorescence assay alkaline phosphatase (ALP) method was developed by using bimetallic lanthanide coordination polymer nanoparticles (Tb-GMP-Eu CPNs).

Carbon dots doped lanthanide coordination polymer nanocomposite as ratiometric fluorescent probe for the sensitive detection of alkaline phosphatase activity

The Analyst ◽  
2021 ◽  
Author(s):  
Weidi Sun ◽  
Xue Han ◽  
Fengli Qu ◽  
Rong-Mei Kong ◽  
Zilong Zhao
Keyword(s):  
Alkaline Phosphatase ◽  
Coordination Polymer ◽  
Polymer Nanocomposite ◽  
Activity Analysis ◽  
Ratiometric Fluorescence ◽  
Fluorescence Sensing ◽  
Lanthanide Coordination Polymer ◽  
Stimulus Responsive ◽  
Ratiometric Fluorescence Sensing ◽  
Ratiometric Fluorescent Probe

Developing sensitive methods for alkaline phosphatase (ALP) activity analysis is an important analytical topic. Based on the stimulus-responsive lanthanide coordination polymer, a simple ratiometric fluorescence sensing strategy was proposed to...

A turn-on fluorescence assay of alkaline phosphatase activity using a DNA–silver nanocluster probe

2018 ◽  
Vol 42 (6) ◽  
pp. 4331-4336 ◽  
Author(s):  
Changbei Ma ◽  
Haisheng Liu ◽  
Kefeng Wu ◽  
Mingjian Chen ◽  
Hailun He ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Assay ◽  
Label Free ◽  
Silver Nanocluster ◽  
Turn On

A label-free fluorescence assay has been developed for the detection of alkaline phosphatase based on DNA–silver nanocluster probes.

Scanning electron microscopy, whole-mount histology, and histochemistry of two anodontine glochidia (Bivalvia: Unionidae)

1996 ◽  
Vol 74 (11) ◽  
pp. 1964-1973 ◽  
Author(s):  
M. Pekkarinen
Keyword(s):  
Methylene Blue ◽  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Neutral Lipids ◽  
Periodic Acid ◽  
Critical Point Drying ◽  
Electrolyte Uptake

Intramarsupial glochidia of Anodonta anatina (L.) and Pseudanodonta complanata (Rossmässler) were studied in southern Finland. Material staining positively with periodic acid – Schiff's reagent (PAS), neutral lipid reserves, and acid phosphatase activity have different distributions in the mantle of the two species. Moreover, the mucous covering of the mantle of the two glochidia behaves differently on critical-point drying. The presence of microvilli with alkaline phosphatase activity on the mantle surface and acid phosphatase activity in the mantle cells in both glochidia suggest that the mantle plays a role in nutrient uptake and digestion and possibly also in electrolyte uptake. The primordia of the stomach, digestive diverticula, and intestine, at least in A. anatina glochidia, contain neutral lipids and exhibit acid phosphatase activity: In A. anatina glochidia, a microvillous layer with alkaline phosphatase activity continues from the ventral walls of the lateral pits to the suspected kidney diverticula. In both glochidia, there may be three pairs of rudimentary ganglia, which do not stain with methylene blue. The eight ciliated sense organs of the glochidia are methylene blue- and PAS-positive and they exhibit succinate dehydrogenase and acid phosphatase activity. In each mantle lobe, the enveloping cell of the dorsal ciliary organ is interconnected with those of the ventral triad via a cellular fold or "tract," and the ciliated central cells of the organs send axons towards each other.

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