Polymer brush based fluorescent immunosensor for direct monitoring of interleukin-1β in rat blood

The Analyst ◽  
2019 ◽  
Vol 144 (19) ◽  
pp. 5682-5690 ◽  
Author(s):  
Fei Deng ◽  
Yi Li ◽  
Md Jakir Hossain ◽  
Michael D. Kendig ◽  
Ria Arnold ◽  
...  
Keyword(s):  
Whole Blood ◽  
Polymer Brush ◽  
Interleukin 1Β ◽  
Rat Blood

A sandwich immunosensor was successfully developed for monitoring of interleukin-1β (IL-1β) in rat whole blood.

scholarly journals PENGARUH CHLOROQUINE TERHADAP INTERLEUKIN-1β, AKTIVASI CASPASE-1 DAN SURVIVAL RATE PADA TIKUS MODEL SEPSIS

Biomedika ◽  
2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Aryo Suseno ◽  
Tatar Sumandjar ◽  
HM Bambang Purwanto
Keyword(s):  
Survival Rate ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Rattus Norvegicus ◽  
Cecal Ligation ◽  
Interleukin 1Β ◽  
Cecal Ligation And Puncture ◽  
Caspase 1

Sepsis masih merupakan salah satu penyebab kematian tertinggi di ruang rawat intensif. Mekanisme sepsis belum diketahui secara penuh. Perlunya metode-metode baru dalam penanganan sepsis. Penelitian-penelitian baru menemukan adanya peran dari Nod-like Receptor dan inflammasome. Chloroquine menunjukkan hasil yang cukup menjanjikan. Penelitian ini bertujuan untuk mengetahui pengaruh qloroquin terhadap interleukin-1β, aktivasi caspase-1 dan survival rate pada tikus model sepsis. Penelitian adalah eksperimen hewan coba (Rattus norvegicus) yang disepsiskan dengan Cecal Ligation and Puncture (CLP). Sampel 52 tikus, dibagi menjadi 2 kelompok; Kelompok 1 untuk IL-1β dan kelompok 2 aktivasi Caspase-1. Tiap kelompok dibagi 3 sub kelompok; kontrol tanpa CLP + placebo (NaCl 0.9% 2ml), perlakuan dengan CLP + placebo, dan kelompok terapi dengan CLP +Chloroquine (CHQ) 50mg/Kg BB personde. Terapi dan placebo diberikan 24, 48 dan 72 Jam setelah CLP. Tikus mati dan moribund dicatat sebagai mortalitas. Pada hari ke 6, tikus yang hidup diambil darahnya dan dikorbankan dengan dislokasi servikal. Kadar IL-1β dengan ELISA pada serum, Aktivasi Caspase-1 dengan Flowcytometry dengan pewarnaan FLICA 660 pada Peripheral Blood Mono-Nuclear Cells (PBMC) dan Whole Blood (WB). Analisis dengan SPSS 22. Beda rerata masing-masing subkelompok dianalisa dengan ANOVA bila distribusi normal dan atau uji Kruskal-Wallis dilanjutkan dengan Mann–Whitney.Hasil penelitian menunjukkan tidak adanya perbedaan survival rate yang bermakna antar sub-kelompok dan antar kelompok (mean 52 vs 62, CI: (-37.32) - 27.98, P=0.767). Didapatkan rerata kadar IL-1β yang lebih rendah pada kelompok terapi dibanding kelompok perlakuan (mean 0.08975 vs 0.09680 CI: (-0.024) – 0.0097, P=0,376), namun tidak bermakna secara statistik. Rerata tingkat aktivasi Caspase-1 pada PBMC (mean 9,46 vs 15.04 CI: (-6.72) – 24.82 , P=0.865) dan WB (mean 2,99 vs 10,99 CI: (-5.303) – 5.844, P=0.478) lebih rendah pada kelompok terapi dibanding perlakuan walaupun tidak bermakna secara statistik. Penelitian ini menunjukkan bahwa penghambatan kadar inflamasi tidak berhubungan langsung dengan survival rate. Mekanisme anti-inflamasi Chloroquine, salah satunya, tampak melewati jalur inflammasome. Kata kunci: Sepsis, Chloroquine, Inflammasome, Caspase-1, IL-1β, Survival rate.

RAPID SCREENING FOR P2X7 ALLELE WHICH MAY REGULATE LPS-INDUCED INTERLEUKIN-1β PRODUCTION IN WHOLE BLOOD

2003 ◽  
Vol 99 (Supplement) ◽  
pp. B19
Author(s):  
Giuditta Angelini ◽  
Loren Denlinger ◽  
Doug Coursin ◽  
Paul Bertics ◽  
Kirk Hogan
Keyword(s):  
Whole Blood ◽  
Interleukin 1Β ◽  
Rapid Screening

scholarly journals Comparison of the Interleukin-1β-Inducing Potency of Allergenic Spores from Higher Fungi (Basidiomycetes) in a Cryopreserved Human Whole Blood System

2013 ◽  
Vol 163 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Félix E. Rivera-Mariani ◽  
Kranthi Vysyaraju ◽  
Jesse Negherbon ◽  
Estelle Levetin ◽  
W. Elliot Horner ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood System ◽  
Interleukin 1Β ◽  
Human Whole Blood

Platelet Aggregation and Fibrinogen Binding in Human, Rhesus Monkey, Guinea-Pig, Hamster and Rat Blood: Activation by ADP and a Thrombin Receptor Peptide and Inhibition by Glycoprotein IIb/IIIa Antagonists

1993 ◽  
Vol 70 (03) ◽  
pp. 531-539 ◽  
Author(s):  
Nigel S Cook ◽  
Hans-Günter Zerwes ◽  
Carlo Tapparelli ◽  
Max Powling ◽  
Jagjit Singh ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Guinea Pig ◽  
Rhesus Monkey ◽  
Whole Blood ◽  
Flow Cytometric Analysis ◽  
Human Platelets ◽  
Thrombin Receptor ◽  
Important Species ◽  
Rat Blood ◽  
Fibrinogen Binding

SummaryPlatelet aggregation and fibrinogen binding in whole blood, induced either by ADP or by a 14 amino acid peptide mimicking an N-terminal region of the platelet thrombin receptor (TRP, thrombin receptor activating peptide), have been studied with blood from different species. Aggregation was assessed by counting the number of single platelets in blood before und after addition of the agonist with an automated cell counter. Both ADP (0.02-0.5 μM) and TRP (1-10 μM) were found to be potent agonists of platelet aggregation in human, rhesus monkey and guinea-pig blood, causing a near-maximal aggregatory response within 2 min of agonist addition. In contrast, hamster and rat platelets were much less responsive to both ADP and TRP in terms of the whole blood aggregation.Echistatin, RGDW and a synthetic glycoprotein (GP) IIb/IIIa antagonist, Ro 43-8857, inhibited fibrinogen binding to purified immobilized human GP-IIb/IIIa with IC50’s of 1.6, 88 and 11.4 nM, respectively. In whole human blood, the respective IC50’s (as determined by flow cytometric analysis of platelet fibrinogen binding) were 4.4, 1700 and 29.5 nM. The affinities of these three compounds for inhibiting fibrinogen binding in whole blood from rhesus monkeys and guinea-pigs were similar to the affinities for human platelets, but with rat blood echistatin, RGDW and Ro 43-8857 were all around 100-fold less potent. Ro 43-8857 was a potent inhibitor of ADP- or TRP-induced platelet aggregation in human, rhesus monkey and guinea-pig whole blood (IC50 of 69-320 nM) but was much less active in hamster blood.These results highlight important species differences in the response of platelets to activation by two different agonists and also in their inhibition by GP-IIb/IIIa antagonists. In particular, platelets from the rat and hamster were insensitive to agonists and antagonists, whereas guinea-pig and rhesus monkey platelets responded with an affinity similar to human platelets. Since these studies were performed in whole blood, the results should be representative of those expected in animal experiments. These recently developed methods for studying platelet responses in small aliquots of whole blood are simple to perform and provide important information concerning the optimal choice of species for subsequent in vivo studies with these compounds.

A novel hydrophilic polymer-brush pattern for site-specific capture of blood cells from whole blood

2015 ◽  
Vol 51 (20) ◽  
pp. 4200-4203 ◽  
Author(s):  
Jianwen Hou ◽  
Qiang Shi ◽  
Wei Ye ◽  
Qunfu Fan ◽  
Hengchong Shi ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Polymer Brush ◽  
Hydrophilic Polymer ◽  
Site Specific

A novel hydrophilic PAMPS–PAAm brush pattern is fabricated to selectively capture blood cells from whole blood.

1α,25-DIHYDROXYVITAMIN D3SUPPRESSES INTERLEUKIN-1β-INDUCED INTERLEUKIN-8 IN HUMAN WHOLE BLOOD: AN INVOLVEMENT OF ERYTHROCYTES IN THE INHIBITION

2002 ◽  
Vol 24 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Katsushi Takahashi ◽  
Hideki Horiuchi ◽  
Tomohiro Ohta ◽  
Keiji Komoriya ◽  
Hitoshi Ohmori ◽  
...  
Keyword(s):  
Whole Blood ◽  
Interleukin 8 ◽  
Interleukin 1Β ◽  
Human Whole Blood

Interleukin-1β (IL-1β), IL-1 receptor antagonist, and TNFα production in whole blood

1992 ◽  
Vol 52 (6) ◽  
pp. 687-692 ◽  
Author(s):  
Judith L. Nerad ◽  
Jeffrey K. Griffiths ◽  
W.M Jos ◽  
Van der Meer ◽  
Stefan Endres ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Whole Blood ◽  
Interleukin 1Β
Sign in / Sign up

Export Citation Format

Share Document