scholarly journals Carbon–carbon double bond position elucidation in fatty acids using ozone-coupled direct analysis in real time mass spectrometry

The Analyst ◽  
2019 ◽  
Vol 144 (19) ◽  
pp. 5848-5855 ◽  
Author(s):  
Nicolas Cetraro ◽  
Robert B. Cody ◽  
Joanne Y. Yew
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Double Bond ◽  
Real Time ◽  
Direct Analysis ◽  
Double Bond Position ◽  
Carbon Double Bond ◽  
Ms Analysis

Ozonolysis combined with DART MS analysis provides a facile method for the identification of double bond position in lipids.

scholarly journals A method to determine the double bond position in unsaturated fatty acids by solvent plasmatization using liquid chromatography-mass spectrometry

2019 ◽  
Author(s):  
Shigeo Takashima ◽  
Kayoko Toyoshi ◽  
Takuhei Yamamoto ◽  
Nobuyuki Shimozawa
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Liquid Chromatography ◽  
Double Bond ◽  
Structural Properties ◽  
Double Bond Position ◽  
Liquid Chromatography Mass Spectrometry ◽  
Carbon Double Bond ◽  
Chromatography Mass Spectrometry ◽  
True Diversity

AbstractFatty acids (FAs) are the central components of life: they constitute biological membranes in the form of lipid, act as signaling molecules, and are used as energy sources. FAs are classified according to their chain lengths and the number and position of carbon-carbon double bond, and their physiological character is largely defined by these structural properties. Determination of the precise structural properties is crucial for characterizing FAs, but pinpointing the exact position of carbon-carbon double bond in FA molecules is challenging. Herein, a new analytical method is reported for determining the double bond position of mono- and poly-unsaturated FAs using liquid chromatography-mass spectrometry (LC-MS) coupled with solvent plasmatization. With the aid of plasma on ESI capirally, epoxidation or peroxidation of carbon-carbon double bond in FAs is facilitated. Subsequently, molecular fragmentation occurs at or beside the epoxidized or peroxidized double bond via collision-induced dissociation (CID), and the position of the double bond is elucidated. In this method, FAs are separated by LC, modified by plasma, fragmented via CID, and detected using a time-of-flight mass spectrometer in a seamless manner such that the FA composition in a mixture can be determined. Our method enables thorough characterization of FA species with distinguishing multiple isomers, and therefore can uncover the true diversity of FAs for their application in food, health, and medical sciences.

scholarly journals Optimization of confined direct analysis in real time mass spectrometry (DART-MS)

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2743-2750 ◽  
Author(s):  
Edward Sisco ◽  
Matthew E. Staymates ◽  
Thomas P. Forbes
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Direct Analysis ◽  
Ms Analysis

Developing an understanding of confined DART-MS analysis.

Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry

2017 ◽  
Vol 35 (2) ◽  
pp. 185 ◽  
Author(s):  
Xiaoming GONG ◽  
Ronghui MA ◽  
Hongtao WANG ◽  
Liqiang GUO ◽  
Kai LI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Real Time ◽  
Ochratoxin A ◽  
Direct Analysis ◽  
Tandem Mass
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