scholarly journals Quantitative analysis of 10 classes of phospholipids by ultrahigh-performance liquid chromatography tandem triple-quadrupole mass spectrometry

The Analyst ◽  
2019 ◽  
Vol 144 (13) ◽  
pp. 3980-3987 ◽  
Author(s):  
Qingxia Huang ◽  
Hehua Lei ◽  
Manyuan Dong ◽  
Huiru Tang ◽  
Yulan Wang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Chain Length ◽  
Acyl Chain ◽  
Triple Quadrupole ◽  
Label Free ◽  
Quadrupole Mass ◽  
Acyl Chain Length ◽  
Ultrahigh Performance Liquid Chromatography

We have developed fast and sensitive label-free quantitation with normalization of acyl chain length to quantify 10 classes of phospholipids by UHPLC-MS.

Simultaneous Quantitative Analysis of Multiple Biotransformation Products of Xian-Ling-Gu-Bao, a Traditional Chinese Medicine Prescription, with Rat Intestinal Microflora by Ultra-Performance Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometry

2020 ◽  
Vol 58 (6) ◽  
pp. 494-503
Author(s):  
Xi-Yang Tang ◽  
Meng-Xue Gao ◽  
Hui-Hui Xiao ◽  
Wei-Jing Yun ◽  
Yi Dai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Intestinal Microflora ◽  
Ultra Performance Liquid Chromatography ◽  
Triple Quadrupole ◽  
Quadrupole Mass ◽  
Medicine Prescription ◽  
Chinese Medicine Prescription ◽  
Biotransformation Products

Abstract Xian-Ling-Gu-Bao (XLGB), a famous traditional Chinese medicine prescription consisted of six herbal medicines, was used for prevention and treatment of osteoporosis in China. As an oral formulation, the multiple components contained in XLGB were inevitably biotransformed by the intestinal microflora before absorption via the gastrointestinal tract. However, the dynamic profiles of biotransformation products of XLGB remain unknown. In this paper, a rapid and sensitive ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry method was developed for the simultaneous quantitative analysis of multiple biotransformation products of XLGB with rat intestinal microflora. For 10 selected quantitative compounds, all calibration curves revealed good linearity (r2 > 0.99) within the sampling ranges considered. The whole intra- and inter-day precisions (as relative standard deviation) of all analytes were <13.5%, and the accuracies (as relative error) were in the range from −11.3 to 11.2%. The lower limits of quantification were 20, 10, 5, 20, 2, 2, 2, 5, 2 and 2 ng/mL for sweroside, timosaponin BII, epimedin C, asperosaponin VI, psoralen, isobavachin, icariside II, timosaponin AIII, isobavachalcone and icaritin, respectively. The matrix effects, extraction recoveries and stabilities were all satisfactory. Meanwhile, dynamic profiles of 21 additional biotransformation products were also monitored by their area-time curves. The analytical method was successfully applied to describe dynamic profiles of 31 biotransformation products of XLGB and the recipes with removal of a definite composed herbal medicine (Anemarrhenae Rhizoma or Rehmanniae Radix).

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