Systematic analysis of enoxaparins from different sources with online one- and two-dimensional chromatography

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3746-3755
Author(s):  
Yilan Ouyang ◽  
Meng Zhu ◽  
Xin Wang ◽  
Lin Yi ◽  
Jawed Fareed ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Low Molecular Weight Heparins ◽  
Systematic Analysis ◽  
Different Sources

Enoxaparin, one of the most important low-molecular-weight heparins (LMWHs), is widely used as a clinical anticoagulant.

Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of a Novel Endolytic Heparinase

2004 ◽  
Vol 36 (12) ◽  
pp. 840-844 ◽  
Author(s):  
Yapeng Chao ◽  
Shaoxiang Xiong ◽  
Xiulan Cheng ◽  
Shijun Qian
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Mass Spectrometric ◽  
Low Molecular Weight ◽  
Low Molecular Weight Heparins ◽  
Mass Spectrometric Evidence ◽  
Different Characteristics ◽  
Different Sources ◽  
Catalytic Characterization

Abstract Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was degraded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccharide components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an Nacetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate-substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

A Perspective on Low Molecular Weight Heparins in the Management of Thrombosis

1993 ◽  
Vol 13 (S 01) ◽  
pp. S5-S11 ◽  
Author(s):  
Debra Hoppensteadt ◽  
Jeanine Walenga ◽  
A Ahsan ◽  
O Iqbal ◽  
W Jeske ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Mechanism Of Action ◽  
Manufacturing Process ◽  
Laboratory Tests ◽  
Low Molecular Weight ◽  
Low Molecular Weight Heparins ◽  
Pharmacological Management

SummaryThe introduction of low molecular weight heparins has added a new dimension to the pharmacological management of thrombotic disorders. Because of different chemical and pharmacological characteristics, due to the manufacturing process, each LMWH should be considered as a distinct entitity and only be used for its given indication. A list of commercially available LMWHs is included. The mechanism of action of the LMWHs and their use in various disorders are discussed. Available laboratory tests for monitoring LMWHs are presented and their limitations pointed out.

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