Widefield frequency domain fluorescence lifetime imaging microscopy (FD-FLIM) for accurate measurement of oxygen gradients within microfluidic devices

The Analyst ◽  
2019 ◽  
Vol 144 (11) ◽  
pp. 3494-3504 ◽  
Author(s):  
Hsiao-Mei Wu ◽  
Tse-Ang Lee ◽  
Ping-Liang Ko ◽  
Wei-Hao Liao ◽  
Tung-Han Hsieh ◽  
...  
Keyword(s):  
Frequency Domain ◽  
Fluorescence Lifetime ◽  
Microfluidic Devices ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Oxygen Gradient ◽  
Lifetime Imaging ◽  
Fast Acquisition ◽  
Oxygen Gradients ◽  
Acquisition Speed

A widefield FD-FLIM system with fast acquisition speed is utilized to accurately characterize oxygen gradient distributions within microfluidic devices.

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