Surface engineering of titanium implants with enzyme-triggered antibacterial properties and enhanced osseointegrationin vivo

2018 ◽  
Vol 6 (48) ◽  
pp. 8090-8104 ◽  
Author(s):  
Zhang Yuan ◽  
Suzhou Huang ◽  
Shaoxiong Lan ◽  
Haizhou Xiong ◽  
Bailong Tao ◽  
...  
Keyword(s):  
Drug Release ◽  
Bacterial Adhesion ◽  
Surface Engineering ◽  
Antibacterial Properties ◽  
Titanium Implants ◽  
Antibacterial Drug ◽  
Effective Inhibition

A catechol-functionalized coating on a Van-loaded Ti implant achieves enhanced osseointegration and effective inhibition of bacterial adhesion and enzyme-triggered antibacterial drug release.

Nano-Surface Modification on Titanium Implants for Drug Delivery

2007 ◽  
Vol 1054 ◽  
Author(s):  
Chang Yao ◽  
Thomas J Webster
Keyword(s):  
Drug Release ◽  
Surface Engineering ◽  
Physical Adsorption ◽  
Titanium Implants ◽  
Orthopedic Implant ◽  
Release Behavior ◽  
Oh Groups ◽  
Chemically Modified ◽  
Drug Release Behavior

ABSTRACTThe surface layer of titanium implants, i.e. titanium dioxide, is responsible for the inertness of titanium-based implants within the human body. However, their cytocompatibility properties and long-term efficacy are limited without further surface engineering since the average functional lifetime of an orthopedic implant is only 10 to 15 years. In this study, an electrochemical method known as anodization was used to create titania nanotubular structures on titanium implant surfaces. These nanotubes were about 60 nm wide (inner diameter) and 200 nm deep. In vitro studies found that anodized surfaces consisting of titania nanotube arrays were favored by bone-forming cells (osteoblasts) compared to unanodized surfaces. These titania nano-tubular structures were utilized here as novel drug release delivery systems. It is proposed that the system designed here can have multi-functional drug release to inhibit infection and wound inflammation while increasing new bone formation. For this purpose, antibiotic drugs (penicillin and streptomycin) were loaded into these nanotubular structures by physical adsorption. To mediate interactions between drug molecules and nanotube walls, anodized titanium nanotubes were modified by silanization to possess amine or methyl groups on their surface instead of −OH groups. Results showed increased hydrophobicity of chemically modified titania nanotubes (methyl > amine > hydroxyl terminated surface). These drug loaded substrates were soaked in phosphate buffered solution in a simulated body environment to determine drug release behavior. Buffer solutions were collected and replaced every day. The eluted drug amounts were measured spectroscopically. Results showed more antibiotic penicillin and streptomycin released from chemically modified nanotubes compared to unanodized titanium substrates; specifically, titania anodized nanotubes functionalized with −OH groups did quite well. In this manner, this study advances titanium currently used in orthopedics to possess drug release behavior which can improve orthopedic implant efficacy.

scholarly journals Toward Bactericidal Enhancement of Additively Manufactured Titanium Implants

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 668
Author(s):  
Yingjing Fang ◽  
Shokouh Attarilar ◽  
Zhi Yang ◽  
Guijiang Wei ◽  
Yuanfei Fu ◽  
...  
Keyword(s):  
Surface Modification ◽  
Structural Design ◽  
Bacterial Adhesion ◽  
Antibacterial Properties ◽  
Structure Design ◽  
Titanium Implants ◽  
Active Metal ◽  
Bactericidal Properties ◽  
Surface Modification Techniques

Implant-associated infections (IAIs) are among the most intractable and costly complications in implant surgery. They can lead to surgery failure, a high economic burden, and a decrease in patient quality of life. This manuscript is devoted to introducing current antimicrobial strategies for additively manufactured (AM) titanium (Ti) implants and fostering a better understanding in order to pave the way for potential modern high-throughput technologies. Most bactericidal strategies rely on implant structure design and surface modification. By means of rational structural design, the performance of AM Ti implants can be improved by maintaining a favorable balance between the mechanical, osteogenic, and antibacterial properties. This subject becomes even more important when working with complex geometries; therefore, it is necessary to select appropriate surface modification techniques, including both topological and chemical modification. Antibacterial active metal and antibiotic coatings are among the most commonly used chemical modifications in AM Ti implants. These surface modifications can successfully inhibit bacterial adhesion and biofilm formation, and bacterial apoptosis, leading to improved antibacterial properties. As a result of certain issues such as drug resistance and cytotoxicity, the development of novel and alternative antimicrobial strategies is urgently required. In this regard, the present review paper provides insights into the enhancement of bactericidal properties in AM Ti implants.

scholarly journals A Concise Review of Silver Diamine Fluoride on Oral Biofilm

2021 ◽  
Vol 11 (7) ◽  
pp. 3232
Author(s):  
Jingyang Zhang ◽  
Sofiya-Roksolana Got ◽  
Iris Xiaoxue Yin ◽  
Edward Chin-Man Lo ◽  
Chun-Hung Chu
Keyword(s):  
Clinical Study ◽  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Clinical Studies ◽  
Antibacterial Properties ◽  
Tooth Surface ◽  
Original Research ◽  
Laboratory Studies ◽  
Oral Biofilm ◽  
Silver Diamine Fluoride

Studies have shown that silver diamine fluoride (SDF) is an effective agent to arrest and prevent dental caries due to its mineralizing and antibacterial properties. While plenty of studies have investigated the mineralizing properties, there are few papers that have examined its antibacterial effect on oral biofilm. The objective of this study was to identify the effect of silver diamine fluoride on oral biofilm. Method: The keywords used were (silver diamine fluoride OR silver diammine fluoride OR SDF OR silver fluoride OR AgF AND biofilm OR plaque). Two reviewers screened the titles and abstracts and then retrieved the full text of the potentially eligible publications. Publications of original research investigating the effect of SDF on oral biofilm were selected for this review. Results: This review included 15 laboratory studies and six clinical studies among the 540 papers identified. The laboratory studies found that SDF could prevent bacterial adhesion to the tooth surface. SDF also inhibited the growth of cariogenic bacteria, including Streptococcus mutans, Lactobacillus acidophilus, Streptococcus sobrinus, Lactobacillus rhamnosus, Actinomyces naeslundii, and Enterococcus faecalis, thus contributing to its success in caries arrest. One clinical study reported a decrease in Streptococcus mutans and Lactobacillus sp. in arrested caries after SDF treatment, and another clinical study found that SDF inhibited the growth of periodontitis microbiota, including Porphyromonas gingivalis, Tannerella forsythia, and Prevotella intermedia/nigrescens. However, three clinical studies reported no significant change in the microbial diversity of the plaque on the tooth after SDF treatment. Moreover, one laboratory study and one clinical research study reported that SDF inhibited the growth of Candida albicans. Conclusion: Not many research studies have investigated the effects of SDF on oral biofilm, although SDF has been used as a caries-arresting agent with antibacterial properties. However, a few publications have reported that SDF prevented bacterial adhesion to the teeth, inhibited the growth of cariogenic and periodontal bacteria, and possessed antifungal properties.

Oxaprozin/poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels: morphological, thermal, swelling, drug release and antibacterial properties

2014 ◽  
Vol 50 (2) ◽  
pp. 906-922 ◽  
Author(s):  
Marija M. Babić ◽  
Katarina M. Antić ◽  
Jovana S. Jovašević Vuković ◽  
Bojan Đ. Božić ◽  
Sladjana Z. Davidović ◽  
...  
Keyword(s):  
Drug Release ◽  
Itaconic Acid ◽  
Antibacterial Properties

Bioactive Surface Engineering of Composite Titanium Implants

2021 ◽  
Vol 57 (9) ◽  
pp. 973-979
Author(s):  
T. S. Petrovskaya ◽  
N. E. Toropkov
Keyword(s):  
Surface Engineering ◽  
Titanium Implants

scholarly journals ANTI-BIOFILM POTENTIAL OF MENTHOL PURIFIED FROM MENTHA PIPERITA L. (MINT)

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
R Ejaz ◽  
S Malik ◽  
M Ahmad ◽  
H ALi ◽  
S Choudhry
Keyword(s):  
Column Chromatography ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Bioactive Compound ◽  
Solvent System ◽  
Mentha Piperita ◽  
Phytochemical Analysis ◽  
Antibacterial Drug ◽  
Biofilm Inhibition ◽  
Maximum Separation

Menthol, a bioactive compound of Mentha piperita (mint) with antibacterial properties was purified by column chromatography to determine its anti-biofilm potential. After phytochemical analysis, TLC was carried out using n-hexane: ethyl acetate: methanol: water (2:2:2:1) as the solvent system for ethanolic extract of mint. TLC achieved the maximum separation of mint constituents with Rf value of 0.68. A purified menthol fraction was obtained after silica gel column chromatography using four different eluting solvents. The menthol obtained was then used to perform biofilm inhibition assay to establish its antibacterial potential. Percentage inhibition was highest for bacillus subtilis (79.4%), as opposed to Pseudomonas aeruginosa (33.6%) and the combination of both bacteria (20%). ELISA reader was used to measure absorbance at 450-620nm and 630 nm. Using 450-620nm filter the values for percentage inhibition lies between 48.6-95% for standard and crude menthol samples. Similarly, at 630nm the values of inhibition lie between 23.4-70.6%. This anti-biofilm property of menthol can be utilized in antibacterial drug formulations.

scholarly journals A systematic review on improving the biocompatibility of titanium implants using nanoparticles

2020 ◽  
Vol 7 ◽  
pp. 31
Author(s):  
Nthabiseng Nhlapo ◽  
Thywill Cephas Dzogbewu ◽  
Olga de Smidt
Keyword(s):  
Systematic Review ◽  
Antibacterial Properties ◽  
Close Correlation ◽  
Titanium Implants ◽  
Bacterial Cells ◽  
Eligibility Criteria ◽  
Current Systematic Review ◽  
Prisma Statement ◽  
Meta Analyses ◽  
Bio Compatibility

An ideal biomaterial should be biointegratable with minimum adverse immune response. Titanium (Ti) and its alloys are widely used biomaterials for manufacturing clinical implants because of their innate biocompatibility. However, the bioinert property of Ti may hinder tissue–implant integration and its bio compatibility nature allows for attachment of bacterial cells on implant surfaces. Nanoparticles (NPs) have been proposed as a possible intervention to overcome these biological shortcomings of Ti-based implants. The aim of the current systematic review was to identify literature that demonstrates enhanced biocompatibility of Ti-based implants by incorporating NPs. Electronic searches were conducted through the PubMed/MEDLINE, ScienceDirect, Web of Science and EBSCOhost databases. Studies published in English were extracted, without restrictions on the year of publication, using the following keywords: ‘biocompatibility’, ‘nanoparticles’, ‘titanium’ and ‘implant’. The guidelines stipulated in the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Statement were followed. A total of 630 articles were identified in the initial search and upon reviewing, 21 articles were selected according to the eligibility criteria. The selected literature showed robust evidence to support the hypothesis that the inclusion of NPs improves biocompatibility of Ti implants. The studies further indicated a close correlation between biocompatibility and antibacterial properties, of which NPs have been proven to characteristically achieve both.

Decreased Bacterial Adhesion to Surface-Treated Titanium

2005 ◽  
Vol 28 (7) ◽  
pp. 718-730 ◽  
Author(s):  
B. Del Curto ◽  
M.F. Brunella ◽  
C. Giordano ◽  
M.P. Pedeferri ◽  
V Valtulina ◽  
...  
Keyword(s):  
Titanium Oxide ◽  
Bacterial Adhesion ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Bacterial Attachment ◽  
Treatment Results ◽  
Bone Implant ◽  
Crystalline Layer ◽  
Progressive Loss ◽  
Modification Treatment

Osteointegrative dental implants are widely used in implantology for their well-known excellent performance once implanted in the host. Remarkable bacterial colonization along the transgingival region may result in a progressive loss of adhesion at gum-implant interface and an increase of the bone area exposed to pathogens. This phenomenon may negatively effect the osteointegration process and cause, in the most severe cases, implant failure. The presence of bacteria at implant site affect the growth of new bone tissue and consequently, the achievement of a mechanically stable bone-implant interface, key parameters for a suitable implant osteointegration. In the present work, a novel surface treatment has been developed and optimized in order to convert the amorphous titanium oxide in a crystalline layer enriched in anatase capable of providing not only antibacterial properties but also of stimulating the precipitation of apatite when placed in simulated body fluid. The collected data have shown that the tested treatment results in a crystalline anatase-type titanium oxide layer able to provide a remarkable decrease in bacterial attachment without negatively effecting cell metabolic activity. In conclusion, the surface modification treatment analyzed in the present study might be an elegant way to reduce the risk of bacterial adhesion and increase the lifetime of the transgingival component in the osteointegrated dental implant.

Minocycline-Modified Pure Titanium Has Good Antibacterial Properties and Improves the Osteogenesis of Bone Marrow Stem Cells

2020 ◽  
Author(s):  
Yancong Zhang ◽  
Linbo Dou ◽  
Fenghe Zhang
Keyword(s):  
Stem Cells ◽  
Bacterial Adhesion ◽  
Antibacterial Properties ◽  
Pure Titanium ◽  
Human Mesenchymal Stem Cells ◽  
Calcium Deposition ◽  
Surface Chemical ◽  
New Bone Formation ◽  
Bioactive Materials ◽  
Adhesion Rate

Abstract Background : The development of an ideal implant material with appropriate antibacterial properties and that improves osteogenesis is essential for the guidance of new bone formation in orthopedic and tooth implant surgeries. In this study, we developed minocycline-modified pure titanium. Methods: We exploited the chemistry of polydopamine (PD) for the coating of minocycline. PD was coated on pure titanium, on which minocycline was subsequently immobilized under certain conditions. Minocycline coating was verified by characterizing the surface chemical composition of the coated Ti sheet and was quantitatively measured by fluorescamine assay. Results: The minocycline-coated pure titanium showed a lower bacterial adhesion rate and supported the spread of the osteogenesis differentiation of human mesenchymal stem cells (hMSCs). A remarkable increase in alkaline phosphatase activity and calcium deposition was found when hMSCs were cultured on minocycline-coated pure titanium for 28 days. Conclusions: The minocycline-coated pure titanium may be optimized as clinically applicable bioactive materials for implant and bone materials.

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