Lipid nanoemulsion passive tumor accumulation dependence on tumor stage and anatomical location: a new mathematical model for in vivo imaging biodistribution studies

2018 ◽  
Vol 6 (44) ◽  
pp. 7306-7316 ◽  
Author(s):  
Marina Arantes Radicchi ◽  
Jaqueline Vaz de Oliveira ◽  
Ana Clara Pova Mendes ◽  
Daniela Mara de Oliveira ◽  
Luís Alexandre Muehlmann ◽  
...  
Keyword(s):  
Mathematical Model ◽  
In Vivo Imaging ◽  
Tumor Tissue ◽  
Tumor Stage ◽  
Anatomical Location ◽  
Nanoparticle Delivery ◽  
Biodistribution Studies ◽  
Lipid Nanoemulsion ◽  
Tumor Accumulation

Nanoparticle delivery to tumor tissue is one of the most important applications of nanomedicine.

Synthesis of a new 18F labeled porphyrin for potential application in positron emission tomography. In vivo imaging and cellular uptake

RSC Advances ◽  
2015 ◽  
Vol 5 (120) ◽  
pp. 99540-99546 ◽  
Author(s):  
Ana V. C. Simões ◽  
Sara M. A. Pinto ◽  
Mário J. F. Calvete ◽  
Célia M. F. Gomes ◽  
Nuno C. Ferreira ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Bladder Tumor ◽  
Radiochemical Purity ◽  
Cell Uptake ◽  
Emission Tomography ◽  
Human Bladder ◽  
Biodistribution Studies ◽  
Biodistribution Data ◽  
Positron Emission

Synthesis, labeling and initial biodistribution studies of a new [18F] radiolabeled meso-tetraphenylporphyrin (radiochemical purity >95%). Includes human bladder tumor cell uptake and biodistribution data.

A near-infrared fluorescent heptamethine indocyanine dye with preferential tumor accumulation for in vivo imaging

Biomaterials ◽  
2010 ◽  
Vol 31 (25) ◽  
pp. 6612-6617 ◽  
Author(s):  
Chao Zhang ◽  
Tao Liu ◽  
Yongping Su ◽  
Shenglin Luo ◽  
Ying Zhu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Tumor Accumulation

scholarly journals PRELIMINARY STRUCTURE–ACTIVITY RELATIONSHIP STUDY OF HEPTAMETHINE INDOCYANINE DYES FOR TUMOR-TARGETED IMAGING

2013 ◽  
Vol 06 (01) ◽  
pp. 1350003 ◽  
Author(s):  
QINYUAN GUO ◽  
SHENGLIN LUO ◽  
QINGRONG QI ◽  
CHUNMENG SHI
Keyword(s):  
In Vivo Imaging ◽  
Near Infrared ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Targeted Imaging ◽  
Structure Activity ◽  
Relationship Study ◽  
Tumor Accumulation ◽  
Sar Study

Our research has identified a couple of near-infrared (NIR) heptamethine indocyanine dyes exhibiting preferential tumor accumulation property for in vivo imaging. On the basis of our foregoing work, we describe here a preliminary structure–activity relationship (SAR) study of 11 related heptamethine indocyanine dyes and several essential requirements of these structures for in vivo tumor-targeted imaging.

scholarly journals Knockdown of microRNA-135b in Mammary Carcinoma by Targeted Nanodiamonds: Potentials and Pitfalls of In Vivo Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 866 ◽  
Author(s):  
Romana Křivohlavá ◽  
Eva Neuhӧferová ◽  
Katrine Q. Jakobsen ◽  
Veronika Benson
Keyword(s):  
Antisense Rna ◽  
Tumor Tissue ◽  
Immune Cell ◽  
Ex Vivo ◽  
Tumor Bearing ◽  
Intravenous Application ◽  
Effective Inhibition ◽  
Tumor Accumulation ◽  
Safe Approach

Nanodiamonds (ND) serve as RNA carriers with potential for in vivo application. ND coatings and their administration strategy significantly change their fate, toxicity, and effectivity within a multicellular system. Our goal was to develop multiple ND coating for effective RNA delivery in vivo. Our final complex (NDA135b) consisted of ND, polymer, antisense RNA, and transferrin. We aimed (i) to assess if a tumor-specific coating promotes NDA135b tumor accumulation and effective inhibition of oncogenic microRNA-135b and (ii) to outline off-targets and immune cell interactions. First, we tested NDA135b toxicity and effectivity in tumorospheres co-cultured with immune cells ex vivo. We found NDA135b to target tumor cells, but it binds also to granulocytes. Then, we followed with NDA135b intravenous and intratumoral applications in tumor-bearing animals in vivo. Application of NDA135b in vivo led to the effective knockdown of microRNA-135b in tumor tissue regardless administration. Only intravenous application resulted in NDA135b circulation in peripheral blood and urine and the decreased granularity of splenocytes. Our data show that localized intratumoral application of NDA135b represents a suitable and safe approach for in vivo application of nanodiamond-based constructs. Systemic intravenous application led to an interaction of NDA135b with bio-interface, and needs further examination regarding its safety.

scholarly journals In vivo imaging of nanoparticle delivery and tumor microvasculature with multimodal optical coherence tomography

2014 ◽  
Vol 5 (6) ◽  
pp. 1731 ◽  
Author(s):  
Jason M. Tucker-Schwartz ◽  
Kelsey R. Beavers ◽  
Wesley W. Sit ◽  
Amy T. Shah ◽  
Craig L. Duvall ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
In Vivo Imaging ◽  
Optical Coherence ◽  
Nanoparticle Delivery ◽  
Tumor Microvasculature

scholarly journals Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery

2019 ◽  
Vol 20 (16) ◽  
pp. 3958 ◽  
Author(s):  
Changhee Park ◽  
Jihye Yoo ◽  
Donghyun Lee ◽  
Seok-young Jang ◽  
Soonmin Kwon ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Blood Circulation ◽  
Tumor Tissue ◽  
Spherical Shape ◽  
Chlorin E6 ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Tumor Accumulation

We fabricated poly (ethylene glycol)-block-polycaprolactone (PEG-b-PCL) nanoemulsion for drug delivery and photodynamic therapy. PEG-b-PCL effectively stabilized the interface between water and soybean oil, and the resulting nanoemulsion was about 220.3 nm in diameter with spherical shape. For photodynamic therapy (PDT), chlorin e6 (Ce6) was loaded into the nanoemulsion as a photosensitizer (PS). These chlorin e6-loaded PEG-PCL nanoemulsions (Ce6-PCL-NEs) showed efficient cellular uptake and, upon laser irradiation, generated singlet oxygen to kill tumor cells. Particularly, Ce6-PCL-NEs showed prolonged blood circulation and about 60% increased tumor accumulation compared to free Ce6 after intravenous injection to 4T1 tumor-bearing mice. These results demonstrate the promising potential of Ce6-PCL-NEs for efficient PDT and in vivo drug delivery to tumor tissue.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load
Sign in / Sign up

Export Citation Format

Share Document