Self-assembled RNAi nanoflowers via rolling circle transcription for aptamer-targeted siRNA delivery

2018 ◽  
Vol 6 (28) ◽  
pp. 4638-4644 ◽  
Author(s):  
Hui Cheng ◽  
Shanni Hong ◽  
Zhili Wang ◽  
Na Sun ◽  
Tengfei Wang ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Tumor Targeting ◽  
Sirna Delivery ◽  
Rolling Circle ◽  
Significant Gene ◽  
Self Assembled

Self-assembled and tumor-targeting RNAi nanoflowers, composed of tandem copies of siRNA, showed significant gene silencing without any transfection agents.

Efficient Self‐Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in v itro

2019 ◽  
Vol 37 (6) ◽  
pp. 588-592 ◽  
Author(s):  
Qian Yao ◽  
Yuqi Chen ◽  
Fan Wu ◽  
Fan Wu ◽  
Chaoxing Liu ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Sirna Delivery ◽  
Rolling Circle ◽  
Dna Nanoparticles ◽  
Self Assembled

scholarly journals Novel fusion peptide‐mediated siRNA delivery using self‐assembled nanocomplex

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyung Ah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Immune Cell ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine. Results Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without overt tissue damage and immune cell infiltration. Conclusions The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

scholarly journals Novel Fusion Peptide-Mediated siRNA Delivery Using Self-Assembled Nanocomplex

2020 ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyungah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
Byeong Hee Hwang
Keyword(s):  
Gene Therapy ◽  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Self Assembled

Abstract Background: Gene therapy using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine.Results: Among the novel fusion peptides and siRNAs, nanocomplexes have outstanding cellular uptake and gene silencing effect in vitro and high stability and retention effect of siRNAs in vivo. Oligo arginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased stability and retention of siRNAs at the site of the tumor. Conclusions: The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene therapy.

Self-assembled DNA nanostructures prepared by rolling circle amplification for the delivery of siRNA conjugates

2014 ◽  
Vol 50 (86) ◽  
pp. 13049-13051 ◽  
Author(s):  
Cheol Am Hong ◽  
Bora Jang ◽  
Eun Hye Jeong ◽  
Hansaem Jeong ◽  
Hyukjin Lee
Keyword(s):  
Large Scale ◽  
Rolling Circle Amplification ◽  
Sirna Delivery ◽  
Dna Nanostructures ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Large Scale Preparation ◽  
Amplification Process ◽  
Scale Preparation ◽  
Self Assembled

Large-scale preparation of DNA nanostructures for siRNA delivery has been achieved by an isothermal enzymatic amplification process.

scholarly journals Novel fusion peptide-mediated siRNA delivery using self-assembled nanocomplex

2021 ◽  
Author(s):  
Yeong Chae Ryu ◽  
Kyungah Kim ◽  
Byoung Choul Kim ◽  
Hui-Min David Wang ◽  
BYEONG HEE HWANG
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Viral Infections ◽  
Sirna Delivery ◽  
Cellular Membrane ◽  
Fusion Peptides ◽  
Genetic Level ◽  
Self Assembled

Abstract Background: Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine.Results: Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nanocomplexes using fusion peptides showed improved and evident cellular uptake and gene silencing of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) via the lipid raft-mediated endocytosis pathway, especially to the HDFn cells of the skin, and all of the fusion peptides were biocompatible. Also, intratumorally injected nanocomplexes had increased retention time of siRNAs at the site of the tumor. Finally, nanocomplexes demonstrated significant in vivo gene silencing effect without immunogenicity.Conclusions: The new nanocomplex strategy could become a safe and efficient platform for the delivery of siRNAs into cells and tissues to treat various target diseases through gene silencing.

scholarly journals Novel layer-by-layer self-assembled peptide nanocarriers for siRNA delivery

RSC Advances ◽  
2017 ◽  
Vol 7 (75) ◽  
pp. 47592-47601 ◽  
Author(s):  
Betül Bozdoğan ◽  
Öznur Akbal ◽  
Ekin Çelik ◽  
Mustafa Türk ◽  
Emir Baki Denkbaş
Keyword(s):  
Gene Silencing ◽  
Sirna Delivery ◽  
Layer By Layer ◽  
Self Assembled

Novel stable diphenylalaninamide peptide based nanocarriers were designed by layer-by-layer polyelectrolyte deposition to load siRNA for gene silencing.

Visible quantum-dot-based targeted siRNA delivery for HIF-1α gene silencing

2014 ◽  
Vol 24 (5) ◽  
pp. 445-451 ◽  
Author(s):  
HongYan Zhu ◽  
JingPing Zhu ◽  
AiMei Xie ◽  
Yong Lin ◽  
BeiBei Zhang ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Gene Silencing ◽  
Sirna Delivery

scholarly journals In vivo gene silencing following non-invasive siRNA delivery into the skin using a novel topical formulation

2014 ◽  
Vol 196 ◽  
pp. 355-362 ◽  
Author(s):  
Vikas Hegde ◽  
Robyn P. Hickerson ◽  
Sitheswaran Nainamalai ◽  
Paul A. Campbell ◽  
Frances J.D. Smith ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Sirna Delivery ◽  
Topical Formulation ◽  
Non Invasive
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