scholarly journals Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore

2018 ◽  
Vol 9 (24) ◽  
pp. 5461-5466 ◽  
Author(s):  
Tian-Bing Ren ◽  
Qian-Ling Zhang ◽  
Dongdong Su ◽  
Xing-Xing Zhang ◽  
Lin Yuan ◽  
...  
Keyword(s):  
New Strategy ◽  
First Time

A new strategy that integrates the targeting group and response moiety together for the preparation of mitochondrial probe was developed. Bioimaging studies have shown that for the first time, the newly designed probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte.

scholarly journals A Multi-User Interactive Coral Reef Optimization Algorithm for Considering Expert Knowledge in the Unequal Area Facility Layout Problem

2021 ◽  
Vol 11 (15) ◽  
pp. 6676
Author(s):  
Lorenzo Salas-Morera ◽  
Laura García-Hernández ◽  
Carlos Carmona-Muñoz
Keyword(s):  
Coral Reef ◽  
Collaborative Design ◽  
Expert Knowledge ◽  
Fitness Function ◽  
Facility Layout ◽  
Layout Problem ◽  
New Strategy ◽  
Problem Instances ◽  
Expert Designer ◽  
First Time

The problem of Unequal Area Facility Layout Planning (UA-FLP) has been addressed by a large number of approaches considering a set of quantitative criteria. Moreover, more recently, the personal qualitative preferences of an expert designer or decision-maker (DM) have been taken into account too. This article deals with capturing more than a single DM’s personal preferences to obtain a common and collaborative design including the whole set of preferences from all the DMs to obtain more complex, complete, and realistic solutions. To the best of our knowledge, this is the first time that the preferences of more than one expert designer have been considered in the UA-FLP. The new strategy has been implemented on a Coral Reef Optimization (CRO) algorithm using two techniques to acquire the DMs’ evaluations. The first one demands the simultaneous presence of all the DMs, while the second one does not. Both techniques have been tested over three well-known problem instances taken from the literature and the results show that it is possible to obtain sufficient designs capturing all the DMs’ personal preferences and maintaining low values of the quantitative fitness function.

scholarly journals Synthesis and characterization of green tea (Camellia sinensis (L.) Kuntze) extract and its major components-based nanoflowers: a new strategy to enhance antimicrobial activity

RSC Advances ◽  
2017 ◽  
Vol 7 (70) ◽  
pp. 44303-44308 ◽  
Author(s):  
Ayşe Baldemir ◽  
N. Buse Köse ◽  
Nilay Ildız ◽  
Selen İlgün ◽  
Sadi Yusufbeyoğlu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Synthesis And Characterization ◽  
New Strategy ◽  
Main Components ◽  
First Time

In this study, for the first time, a novel organic–inorganic nanobio-antimicrobial agent called “nanoflowers” (Nfs) from Camellia sinensis (L.) Kuntze extracts and its main components were produced and the increase in the antimicrobial activity of Nfs was elucidated.

scholarly journals Aligning policy and law? The creation of a domestic abuse offence incorporating coercive control

2018 ◽  
Vol 18 (1) ◽  
pp. 67-83 ◽  
Author(s):  
Michele Burman ◽  
Oona Brooks-Hay
Keyword(s):  
Criminal Justice ◽  
Domestic Abuse ◽  
Unintended Consequences ◽  
Access To Justice ◽  
Coercive Control ◽  
New Strategy ◽  
Scottish Government ◽  
Definition Of ◽  
First Time ◽  
Criminal Justice Response

Since 2000, the Scottish Government has adopted a gendered definition of domestic abuse which explicitly positions it as both a cause and a consequence of gender inequality. Following the launch of a new strategy to prevent and eradicate violence against women and girls, the Scottish Government announced proposals to create, for the first time, a bespoke offence of domestic abuse, designed to encompass the spectrum of abusive acts that constitute domestic abuse, including emotional and psychological abuse. The new offence is intended to better reflect the experience of victims subject to coercive control, improve the criminal justice response and facilitate access to justice. It represents one of the most radical attempts yet to align the criminal justice response with contemporary policy and feminist conceptual understanding of domestic abuse as a form of coercive control. Drawing on feminist scholarship which has interrogated the value of law reform, we critically assess the scope of the legislation, the likely challenges associated with its use in the Scottish context, and the potential for unintended consequences.

Intratumoral injection of oncolytic adenovirus H101 in combination with vinorelbine/cisplatin chemotherapy in patients with advanced non-small cell lung cancer

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 17082-17082
Author(s):  
C. Zhou ◽  
Y. Xu ◽  
J. Ni ◽  
S. Zhou ◽  
J. Xu
Keyword(s):  
Advanced Nsclc ◽  
Survival Rates ◽  
Intratumoral Injection ◽  
Oncolytic Adenovirus ◽  
Time To Progression ◽  
Minor Response ◽  
Viral Particles ◽  
New Strategy ◽  
Nsclc Patients ◽  
First Time

17082 Background: Chemotherapy is main treatment for advanced NSCLC. But its efficacy is quite small with improvement of 1–2 months in median survival. New strategy against NSCLC is needed. Oncolytic adenovirus H101 is found to be effective against NSCLC. This trial is desigened to investigate efficacy and toxicity of intratumoral injection of H101 in combination with chemotherapy in the treatment of NSCLC. Methods: The NSCLC patients confirmed cytologically or pathologically were randomized to intratumoral injection of adenovirus H101 (1.5 × 1012 viral particles) plus NP chemotherapy (arm A) or NP chemotherapy (arm B). Objetive response was evaluated every two cycles and time to progression (TTP) and overall survival were followed up. Results: Out of 18 evaluable patients in Arm A, 4 patients showed partial response (PR),3 minor response(MR), 7 stable disease (SD) and 4 disease progression (PD), while in Arm B 16 evaluable patients showed 3 PR, 4 MR, 3 SD and 6 PD. At the first time of response evaluation, there was 1 PD in Arm A,but in Arm B there were 5 of PD. Failure rate in Arm B was significantly higher than in Arm B. Survival curves between the two arms were similar. Six month, 9 month and 1 year survival rates were slightly higher in Arm B and median TTP was also prolonged in Arm A. Except non-infectious fever, Arm A was similar in other toxicities to Arm B. there was only 2 patients developing mild pneumothorax. Conclusions: Intratumoral injection of H101 1.5 × 1012 viral particles in combination with NP chemotherapy was feasible, effective and safe in treatment of advanced NSCLC. No significant financial relationships to disclose.

scholarly journals High Performance of Simple Organic Phosphorescence Host-Guest Materials

2020 ◽  
Author(s):  
Yunsheng Wang ◽  
Heqi Gao ◽  
Jie Yang ◽  
Manman Fang ◽  
Dan Ding ◽  
...  
Keyword(s):  
High Performance ◽  
High Efficiency ◽  
Aqueous Media ◽  
Theoretical Research ◽  
Long Afterglow ◽  
Afterglow Time ◽  
New Strategy ◽  
Long Lifetime ◽  
First Time ◽  
Design Guidance

<p><b>The study of purely organic room-temperature phosphorescence (RTP) has drawn </b><b>increasing attention </b><b>because of its considerable theoretical research and practical application </b><b>value</b><b>. Currently, organic RTP materials with both high efficiency (<i>Ф</i><sub>P</sub> > 20%) and a long lifetime (<i>τ</i><sub>P</sub> > 10 s) in air are still </b><b>scarce due to</b><b> the lack of related design guidance. Here, we </b><b>report</b><b> a new strategy to increase the phosphorescence performance of organic materials by integrating the RTP host and RTP guest in one doping system to form </b><b>a </b><b>triplet exciplex. With these materials, the high-contrast </b><b>labelling</b><b> of tumours in living mice and encrypted patterns in thermal printing are both successfully realized for the first time</b><b> by</b><b> taking </b><b>advantage of both</b><b> the long afterglow time (up to 25 min in aqueous media) and high phosphorescence efficiency (43%).</b></p>

New strategy to achieve La2O2CN2:Eu3+ novel luminescent one-dimensional nanostructures

CrystEngComm ◽  
2014 ◽  
Vol 16 (24) ◽  
pp. 5409-5417 ◽  
Author(s):  
Xiaomin Guo ◽  
Jinxian Wang ◽  
Xiangting Dong ◽  
Wensheng Yu ◽  
Guixia Liu
Keyword(s):  
Luminescence Properties ◽  
One Dimensional ◽  
New Strategy ◽  
First Time ◽  
One Dimensional Nanostructures

For the first time, La2O2CN2:Eu3+ nanofibers and nanobelts were successfully prepared and exhibit excellent luminescence properties.

scholarly journals High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis

2017 ◽  
Author(s):  
Wanxin Wang ◽  
Lolita Penland ◽  
Ozgun Gokce ◽  
Derek Croote ◽  
Stephen Quake
Keyword(s):  
Single Cell ◽  
Organ Transplant ◽  
Population Heterogeneity ◽  
High Fidelity ◽  
Intact Mouse ◽  
New Strategy ◽  
Cell Transcriptome ◽  
Definition Of ◽  
Single Cell Transcriptome ◽  
First Time

AbstractBackgroundHigh-fidelity preservation strategies for primary tissues are in great demand in the single cell RNAseq community. A reliable method will greatly expand the scope of feasible collaborations and maximize the utilization of technical expertise. When choosing a method, standardizability is as important a factor to consider as fidelity due to the susceptibility of single-cell RNAseq analysis to technical noises. Existing approaches such as cryopreservation and chemical fixation are less than ideal for failing to satisfy either or both of these standards.ResultsHere we propose a new strategy that leverages preservation schemes developed for organ transplantation. We evaluated the strategy by storing intact mouse kidneys in organ transplant preservative solution at hypothermic temperature for up to 4 days (6 hrs, 1, 2, 3, and 4 days), and comparing the quality of preserved and fresh samples using FACS and single cell RNAseq. We demonstrate that the strategy effectively maintained cell viability, transcriptome integrity, cell population heterogeneity, and transcriptome landscape stability for samples after up to 3 days of preservation. The strategy also facilitated the definition of the diverse spectrum of kidney resident immune cells, to our knowledge the first time at single cell resolution.ConclusionsHypothermic storage of intact primary tissues in organ transplant preservative maintains the quality and stability of the transcriptome of cells for single cell RNAseq analysis. The strategy is readily generalizable to primary specimens from other tissue types for single cell RNAseq analysis.

scholarly journals High Internal-Phase Pickering Emulsions Stabilized by Xanthan Gum/Lysozyme Nanoparticles: Rheological and Microstructural Perspective

2022 ◽  
Vol 8 ◽  
Author(s):  
Wei Xu ◽  
Zhifan Li ◽  
Haomin Sun ◽  
Shuqing Zheng ◽  
He Li ◽  
...  
Keyword(s):  
Xanthan Gum ◽  
Colloidal Particles ◽  
Physical Stability ◽  
Pickering Emulsions ◽  
High Concentration ◽  
Internal Phase ◽  
New Strategy ◽  
Emulsion Systems ◽  
The Common ◽  
First Time

Food-grade high internal-phase Pickering emulsions (HIPPEs) stabilized by solid or colloidal particles with different advantages have attracted extensive attention nowadays. However, looking for new appropriate particle stabilizers is the common practice for HIPPEs preparation. It is crucial to find a new strategy for the development of functional HIPPEs with controllable properties. In this study, a high concentration of xanthan gum/lysozyme nanoparticles (XG/Ly NPs) was used for the preparation of HIPPEs for the first time. Visual observations, creaming index (CI), microstructure, and rheology tests were carried out to investigate the potential of XG/Ly NPs as HIPPEs stabilizers. Results indicated that XG/Ly NPs could stabilize oil droplets in the concentration range of 0.5–4% (w/v). The HIPPEs with a minimal particle concentration of 1% exhibited remarkable physical stability. Rheological measurements showed that a high stability of solid-like HIPPEs was successfully obtained with a higher concentration of XG/Ly NPs. Overall, the HIPPEs stabilized by different concentrations of XG/Ly NPs exhibited excellent rheological and structural properties, which might provide a feasible strategy for the development of functional emulsion systems with controllable structures.

Efficient Access to Aliphatic Esters by Photocatalyzed Alkoxycarbonylation of Alkenes

2021 ◽  
Author(s):  
Jian-Qiang Chen ◽  
Xiaodong Tu ◽  
Qi Tang ◽  
Ke Li ◽  
Liang Xu ◽  
...  
Keyword(s):  
Natural Product ◽  
Oxalyl Chloride ◽  
Biologically Active ◽  
Formal Synthesis ◽  
Efficient Access ◽  
Wide Range ◽  
New Strategy ◽  
Aliphatic Esters ◽  
First Time

Abstract Aliphatic esters are essential constituents of biologically active compounds and versatile chemical intermediates for the synthesis of drugs. However, their preparation from readily available olefins remains challenging. In this report, a new strategy to access aliphatic esters from olefins through a unique photocatalyzed alkoxycarbonylation reaction is described. Alkyloxalyl chlorides, generated in situ from the corresponding alcohols and oxalyl chloride, are engaged for the first time as alkoxycarbonyl radical fragments under photoredox catalysis. This transformation tolerates a broad scope of electron-rich and electron-deficient olefins and provides the corresponding β-chloro esters in good yields. Additionally, a formal β-selective alkene alkoxycarbonylation is developed. And, a variety of oxindole-3-acetates and furoindolines are prepared in good to excellent yields. A more concise formal synthesis of (±)-physovenine is accomplished as well. With these strategies, a wide range of natural-product-derived olefins and alkyloxalyl chlorides are also successfully employed.

Unexpected Evidence That Dimethylsulphoxide (DMSO) Upregulates Expression of CXCR4 on Hematopoietic Stem/Progenitor Cells (HSPC), Increases Their Responsiveness to an SDF-1 Gradient and Enhances Homing to Bone Marrow.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1973-1973
Author(s):  
Marcin Majka ◽  
Danuta Jarocha ◽  
Marcin Wysoczynski ◽  
Duygu Sag ◽  
Ewa Zuba-Surma ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mononuclear Cells ◽  
Short Exposure ◽  
Hematopoietic Stem ◽  
Akt Phosphorylation ◽  
Human Cd34 ◽  
New Strategy ◽  
First Time

Abstract Cryopreservation of bone marrow (BM), mobilized peripheral blood (mPB) and cord blood (CB) cells is a routine procedure to store hematopoietic stem/progenitor cells (HSPC) for transplantation. Dimethylsulphoxide (DMSO), the most commonly used cryoprotectant, is toxic to cells at higher concentrations (>10%); moreover, the freezing-thawing procedure itself is inevitably connected with the loss of HSPC. However, by chance we observed that short exposure of HSPC to DMSO enhances the responsiveness of these cells to an SDF-1 gradient and since SDF-1 is a major chemoattractant that navigates homing of HSPC to BM we became interested in elucidating this phenomenon. We found that short incubation (5–10 min) of human CB mononuclear cells (MNC) with DMSO at concentrations employed for cryopreservation (5–10%) significantly upregulates the expression of both CXCR4 (x 2–3) and CD34 (x 1.5) on CB MNC (as measured by FACS). Furthermore, DMSO significantly increased the chemotactic responsiveness (x 2–4) of CB MNC, BM MNC and selected CXCR4+ human hematopoietic cell lines (Jurkat, THP-1 cells) when the cells were exposed to 5–10% DMSO before chemotaxis assay. These responses to an SDF-1 gradient correlated with enhanced chemotaxis also of human CD34+, CD34+ CD38+, CD34+ CD38−, and CD34+ CXCR4+ clonogeneic progenitor cells, suggesting that DMSO directly enhances the responsiveness of human early progenitors (p<0.0001). At the molecular level, 5–10% DMSO strongly stimulated and prolonged SDF-1-dependent AKT phosphorylation. However, at the same time DMSO inhibited phosphorylation of MAPKp42/44. Similar observations were made for Sca-1+ BM-derived murine cells. In parallel experiments we found that murine Sca-1+ cells when preincubated with DMSO formed more 12 day-CFU-S colonies in spleens after transplantation into irradiated syngeneic recipients. Accordingly, x 2 more CFU-S were formed when Sca-1+ cells were exposed before transplantation to 5% DMSO and about x 4 more after exposure to 10% DMSO. Finally we employed a Ly5.1/Ly5.2 congeneic transplant model and showed that transplantation of Ly5.1 Sca-1+ cells exposed to 10% DMSO before transplantation resulted in higher chimerism in transplanted Ly5.2 mice as compared to untreated cells (control) (p<0.0001). In conclusion, we show for the first time an unexpected beneficial role of DMSO (5–10%) in regulation of homing of HSPC after transplantation and suggest that a short priming of HSPC with DMSO, even of non-cryopreserved cells, before transplantation may become a new strategy to enhance engraftment

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