scholarly journals Fabrication of an immunosensor for quantitative detection of breast cancer biomarker UBE2C

RSC Advances ◽  
2019 ◽  
Vol 9 (29) ◽  
pp. 16738-16745 ◽  
Author(s):  
V. S. P. K. Sankara Aditya Jayanthi ◽  
Asim Bikas Das ◽  
Urmila Saxena
Keyword(s):  
Breast Cancer ◽  
Modified Electrode ◽  
Cancer Biomarker ◽  
Quantitative Detection

Antibodies crosslinking on PANI modified electrode to design a highly selective immunosensor to detect UBE2C.

scholarly journals Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection

2021 ◽  
Vol 255 ◽  
pp. 13003
Author(s):  
Tommaso Pileri ◽  
Alberto Sinibaldi ◽  
Agostino Occhicone ◽  
Elena Giordani ◽  
Matteo Allegretti ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Specific Interaction ◽  
Fluorescence Emission ◽  
Optical Biosensor ◽  
Cancer Biomarker ◽  
Time Development ◽  
Biomarker Detection ◽  
Label Free ◽  
One Dimensional ◽  
Protein Bovine Serum Albumin

An optical biosensor for proteomic breast cancer biomarker detection in complex media is presented. Bloch Surface Waves (BSW) excited onto one dimensional photonic crystal (1DPC) were used to probe the interaction of HER2 with three antibody species and an inert protein (Bovine Serum Albumin - BSA). The optical system combines Label-Free readings to track the bioassay real-time development and Fluorescence emission quantification to evaluate the level of specific interaction between the antigen and the antibodies. The results confirm a distinguishable level of affinity between the antibodies and the analyte according to their specificity even at low antibody surface density (about 1173 pg/mm2).

scholarly journals Biocompatible Electrochemical Sensor Based on Platinum-Nickel Alloy Nanoparticles for In Situ Monitoring of Hydrogen Sulfide in Breast Cancer Cells

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 258
Author(s):  
Asit Kumar Panda ◽  
Murugan Keerthi ◽  
Rajalakshmi Sakthivel ◽  
Udesh Dhawan ◽  
Xinke Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hydrogen Sulfide ◽  
Electrochemical Sensor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
In Situ Monitoring ◽  
Quantitative Detection ◽  
Alloy Nanoparticles ◽  
Ptni Alloy

Hydrogen sulfide (H2S), an endogenous gasotransmitter, is produced in mammalian systems and is closely associated with pathological and physiological functions. Nevertheless, the complete conversion of H2S is still unpredictable owing to the limited number of sensors for accurate and quantitative detection of H2S in biological samples. In this study, we constructed a disposable electrochemical sensor based on PtNi alloy nanoparticles (PtNi NPs) for sensitive and specific in situ monitoring of H2S released by human breast cancer cells. PtNi alloy NPs with an average size of 5.6 nm were prepared by a simple hydrothermal approach. The conversion of different forms of sulfides (e.g., H2S, HS−, and S2−) under various physiological conditions hindered the direct detection of H2S in live cells. PtNi NPs catalyze the electrochemical oxidation of H2S in a neutral phosphate buffer (PB, pH 7.0). The PtNi-based sensing platform demonstrated a linear detection range of 0.013–1031 µM and the limit of detection was 0.004 µM (S/N = 3). Moreover, the PtNi sensor exhibited a sensitivity of 0.323 μA μM−1 cm−2. In addition, the stability, repeatability, reproducibility, and anti-interference ability of the PtNi sensor exhibited satisfactory results. The PtNi sensor was able to successfully quantify H2S in pond water, urine, and saliva samples. Finally, the biocompatible PtNi electrode was effectively employed for the real-time quantification of H2S released from breast cancer cells and mouse fibroblasts.

scholarly journals HER2 breast cancer biomarker detection using a sandwich optical fiber assay

Talanta ◽  
2021 ◽  
Vol 221 ◽  
pp. 121452 ◽  
Author(s):  
Médéric Loyez ◽  
Maxime Lobry ◽  
Eman M. Hassan ◽  
Maria C. DeRosa ◽  
Christophe Caucheteur ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Optical Fiber ◽  
Cancer Biomarker ◽  
Biomarker Detection ◽  
Her2 Breast Cancer
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