scholarly journals Tolerance against butanol stress by disrupting succinylglutamate desuccinylase inEscherichia coli

RSC Advances ◽  
2019 ◽  
Vol 9 (21) ◽  
pp. 11683-11695 ◽  
Author(s):  
Yuan Guo ◽  
Bo Lu ◽  
Hongchi Tang ◽  
Dewu Bi ◽  
Zhikai Zhang ◽  
...  
Keyword(s):  
Inescherichia Coli ◽  
Butanol Stress

The four-carbon alcohol, butanol, is emerging as a promising biofuel and efforts have been undertaken to improve several microbial hosts for its production.

scholarly journals Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation inEscherichia coli

2019 ◽  
Vol 116 (8) ◽  
pp. 2074-2086 ◽  
Author(s):  
Gavin Kurgan ◽  
Christian Sievert ◽  
Andrew Flores ◽  
Aidan Schneider ◽  
Thomas Billings ◽  
...  
Keyword(s):  
Experimental Evolution ◽  
Xylose Fermentation ◽  
Inescherichia Coli

scholarly journals Butanol Tolerance of Lactiplantibacillus plantarum: A Transcriptome Study

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 181
Author(s):  
Kaloyan Petrov ◽  
Alexander Arsov ◽  
Penka Petrova
Keyword(s):  
Bacterial Species ◽  
Transcriptional Regulators ◽  
Common Species ◽  
Glycerol Metabolism ◽  
Phosphotransferase System ◽  
Promising Alternative ◽  
Butanol Tolerance ◽  
Polysaccharide Biosynthesis ◽  
Major Factors ◽  
Butanol Stress

Biobutanol is a promising alternative fuel with impaired microbial production thanks to its toxicity. Lactiplantibacillus plantarum (L. plantarum) is among the few bacterial species that can naturally tolerate 3% (v/v) butanol. This study aims to identify the genetic factors involved in the butanol stress response of L. plantarum by comparing the differential gene expression in two strains with very different butanol tolerance: the highly resistant Ym1, and the relatively sensitive 8-1. During butanol stress, a total of 319 differentially expressed genes (DEGs) were found in Ym1, and 516 in 8-1. Fifty genes were upregulated and 54 were downregulated in both strains, revealing the common species-specific effects of butanol stress: upregulation of multidrug efflux transporters (SMR, MSF), toxin-antitoxin system, transcriptional regulators (TetR/AcrR, Crp/Fnr, and DeoR/GlpR), Hsp20, and genes involved in polysaccharide biosynthesis. Strong inhibition of the pyrimidine biosynthesis occurred in both strains. However, the strains differed greatly in DEGs responsible for the membrane transport, tryptophan synthesis, glycerol metabolism, tRNAs, and some important transcriptional regulators (Spx, LacI). Uniquely upregulated in the butanol-resistant strain Ym1 were the genes encoding GntR, GroEL, GroES, and foldase PrsA. The phosphoenolpyruvate flux and the phosphotransferase system (PTS) also appear to be major factors in butanol tolerance.

Sign in / Sign up

Export Citation Format

Share Document