scholarly journals Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells

RSC Advances ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 3176-3184 ◽  
Author(s):  
Richard P. Harrison ◽  
Veeren M. Chauhan ◽  
David Onion ◽  
Jonathan W. Aylott ◽  
Virginie Sottile
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Fluorescence Microscopy ◽  
Iron Nanoparticles ◽  
Human Mesenchymal Stem Cells ◽  
Super Resolution ◽  
Ph Sensitive ◽  
Intracellular Processing ◽  
Intracellular Fate

Elucidating the intracellular fate of silica-coated superparamagnetic iron nanoparticles (SiMAGs) using fluorescent pH-sensitive nanosensors, super-resolution fluorescence microscopy and particle counting flow cytometry.

Human Bone Marrow Mesenchymal Stem Cells Enhance Extramedullary Engraftment and Proliferation of Human AML Blasts in NOD-SCID Mice.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2312-2312
Author(s):  
Dean A. Lee ◽  
William C. Choi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Peripheral Blood ◽  
Human Mesenchymal Stem Cells ◽  
Tumor Burden ◽  
Scid Mice ◽  
Mouse Bone Marrow ◽  
Mouse Tumor

Abstract BACKGROUND: Inoculation of human normal or leukemic myeloblasts into sublethaly irradiated NOD/SCID mice often results in persistent low-level engraftment (< 5%), but significant proliferation (≥ 5-fold expansion) rarely occurs. Most malignant samples that engraft and proliferate are of FAB M4 subtype and exhibit rapid extramedullary growth at the site of injection without significant marrow or spleen involvement. We hypothesized that low engraftment and proliferation of less mature FAB subtypes results from an increased requirement of these cells for a marrow environment of cytokine and contact-dependent growth and survival factors not adequately provided across species by the mouse bone marrow stroma. Here we show that the subcutaneous injection of minimally-differentiated human mesenchymal stem cells (MSC) in a Matrigel matrix creates an artificial human marrow environment resulting in improved survival and proliferation of human myeloblasts. METHODS: Human leukemic myeloblasts were obtained from the marrow or peripheral blood of 14 newly diagnosed pediatric patients under an IRB-approved collection and banking protocol. MSC were obtained from sterile filters following processing of human marrow from healthy donors or from the NIH-funded MSC bank at Tulane University. 6-to-12 week old NOD-SCID mice were injected IV with 5x106 AML blasts via the retro-orbital sinus (N=38), subcutaneously in 0.5mL Matrigel (N=18), or subcutaneously with 5x105 MSC in 0.5mL of Matrigel (N=14). Mice were euthanized when evidence of tumor burden was present. Peripheral blood, bone marrow, spleen, and subcutaneous nodules were obtained for flow immunophenotyping, FISH, and histopathology. Percent engraftment was determined by flow cytometry for human CD33-APC and mouse H2Kd-PE. RESULTS: Median time from injection to necropsy was 12.5 weeks. 18% died of spontaneous murine thymomas. No animals died of progressive human AML if myeloblasts were injected IV or subcutaneously with Matrigel, and all had < 5% involvement of bone marrow, spleen, and blood. Six animals injected with AML and MSC (43%) developed visible tumors at a median of 8.5 weeks. These tumors were easily reduced to single cell suspensions of > 98% CD33+ by flow cytometry, with mean estimated recovery of 1.3x108 human myeloblasts per mouse tumor (mean 36-fold expansion, range 4 to 52-fold). For cases in which the AML and MSC were derived from subjects of disparate gender, the origin of the cells (leukemic donor vs. MSC donor) was validated by FISH for human X/Y chromosomes. Histopathology of the resulting mass revealed the central development of a stromal chondroid matrix similar to trabecular bone. Marrow, spleen, and blood for all these animals contained < 5% human myeloblasts. CONCLUSIONS: Here we describe an effective method for expanding immature human leukemic myeloblasts in the NOD/SCID mouse. These findings suggest that less mature myeloblasts require human MSC for survival and proliferation and appear to lack significant homing to or expansion in mouse marrow even in the presence of a significant ectopic tumor burden. This is a useful technique for expanding human AML cells for research, may be a model for more broad-based patient-oriented testing of chemotherapeutic and biologic therapies for AML, and represents a novel animal model for studying the stromal interactions and growth requirements of malignant and non-malignant myeloid precursors.

scholarly journals Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells

2013 ◽  
Vol 36 (4) ◽  
pp. 731-741 ◽  
Author(s):  
Alexander K. C. Chan ◽  
Thomas R. J. Heathman ◽  
Karen Coopman ◽  
Christopher J. Hewitt
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Multiparameter Flow Cytometry

scholarly journals Human mesenchymal stem cells: From immunophenotyping by flow cytometry to clinical applications

2012 ◽  
Vol 83A (1) ◽  
pp. 48-61 ◽  
Author(s):  
Arthur A. Nery ◽  
Isis C. Nascimento ◽  
Talita Glaser ◽  
Vinicius Bassaneze ◽  
José E. Krieger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Clinical Applications

Investigating the Spatial Distribution of Integrin β1 in Patterned Human Mesenchymal Stem Cells Using Super-Resolution Imaging

2014 ◽  
Vol 6 (18) ◽  
pp. 15686-15696 ◽  
Author(s):  
Ajay Tijore ◽  
Srivats Hariharan ◽  
Haiyang Yu ◽  
Chee Ren Ivan Lam ◽  
Feng Wen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spatial Distribution ◽  
Human Mesenchymal Stem Cells ◽  
Super Resolution ◽  
Integrin Β1 ◽  
Resolution Imaging

scholarly journals IL-1β mediated nanoscale surface clustering of integrin α5β1 regulates the adhesion of mesenchymal stem cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stephanie A. Maynard ◽  
Ekaterina Pchelintseva ◽  
Limor Zwi-Dantsis ◽  
Anika Nagelkerke ◽  
Sahana Gopal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Spatial Organization ◽  
Human Mesenchymal Stem Cells ◽  
Super Resolution ◽  
Integrin Α5β1 ◽  
Cell Matrix ◽  
Cluster Area ◽  
Rapid Clearance ◽  
Resolution Imaging

AbstractClinical use of human mesenchymal stem cells (hMSCs) is limited due to their rapid clearance, reducing their therapeutic efficacy. The inflammatory cytokine IL-1β activates hMSCs and is known to enhance their engraftment. Consequently, understanding the molecular mechanism of this inflammation-triggered adhesion is of great clinical interest to improving hMSC retention at sites of tissue damage. Integrins are cell–matrix adhesion receptors, and clustering of integrins at the nanoscale underlies cell adhesion. Here, we found that IL-1β enhances adhesion of hMSCs via increased focal adhesion contacts in an α5β1 integrin-specific manner. Further, through quantitative super-resolution imaging we elucidated that IL-1β specifically increases nanoscale integrin α5β1 availability and clustering at the plasma membrane, whilst conserving cluster area. Taken together, these results demonstrate that hMSC adhesion via IL-1β stimulation is partly regulated through integrin α5β1 spatial organization at the cell surface. These results provide new insight into integrin clustering in inflammation and provide a rational basis for design of therapies directed at improving hMSC engraftment.

scholarly journals Functional investigations on human mesenchymal stem cells exposed to magnetic fields and labeled with clinically approved iron nanoparticles

2010 ◽  
Vol 11 (1) ◽  
Author(s):  
Richard Schäfer ◽  
Rüdiger Bantleon ◽  
Rainer Kehlbach ◽  
Georg Siegel ◽  
Jakub Wiskirchen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Magnetic Fields ◽  
Iron Nanoparticles ◽  
Human Mesenchymal Stem Cells

scholarly journals Prospective isolation and identification of human mesenchymal stem cells by flow cytometry

2009 ◽  
Vol 29 (1) ◽  
pp. 73-78
Author(s):  
Yo Mabuchi ◽  
Satoru Morikawa ◽  
Sadafumi Suzuki ◽  
Takehiko Sunabori ◽  
Hideyuki Okanoa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Isolation And Identification ◽  
Prospective Isolation

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
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