scholarly journals A targeted metabolomic protocol for quantitative analysis of volatile organic compounds in urine of children with celiac disease

RSC Advances ◽  
2018 ◽  
Vol 8 (64) ◽  
pp. 36534-36541 ◽  
Author(s):  
Natalia Drabińska ◽  
Hafiz Abdul Azeem ◽  
Urszula Krupa-Kozak
Keyword(s):  
Celiac Disease ◽  
Quantitative Analysis ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Design Of Experiment ◽  
Urinary Biomarkers ◽  
Volatile Organic

An SPME-GCMS method was optimized for quantitative analysis of 15 urinary biomarkers in celiac disease children using multivariate design of experiment.

scholarly journals Different Fecal Microbiotas and Volatile Organic Compounds in Treated and Untreated Children with Celiac Disease

2009 ◽  
Vol 75 (12) ◽  
pp. 3963-3971 ◽  
Author(s):  
Raffaella Di Cagno ◽  
Carlo G. Rizzello ◽  
Francesca Gagliardi ◽  
Patrizia Ricciuti ◽  
Maurice Ndagijimana ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Lactobacillus Delbrueckii ◽  
Rrna Gene ◽  
Healthy Children ◽  
Fecal Samples ◽  
Dgge Analysis ◽  
Volatile Organic

ABSTRACTThis study aimed at investigating the fecal microbiotas of children with celiac disease (CD) before (U-CD) and after (T-CD) they were fed a gluten-free diet and of healthy children (HC). Brothers or sisters of T-CD were enrolled as HC. Each group consisted of seven children. PCR-denaturing gradient gel electrophoresis (DGGE) analysis with V3 universal primers revealed a unique profile for each fecal sample. PCR-DGGE analysis with group- or genus-specific 16S rRNA gene primers showed that theLactobacilluscommunity of U-CD changed significantly, while the diversity of theLactobacilluscommunity of T-CD was quite comparable to that of HC. Compared to HC, the ratio of cultivable lactic acid bacteria andBifidobacteriumtoBacteroidesand enterobacteria was lower in T-CD and even lower in U-CD. The percentages of strains identified as lactobacilli differed as follows: HC (ca. 38%) > T-CD (ca. 17%) > U-CD (ca. 10%).Lactobacillus brevis,Lactobacillus rossiae, andLactobacillus pentosuswere identified only in fecal samples from T-CD and HC.Lactobacillus fermentum,Lactobacillus delbrueckiisubsp.bulgaricus, andLactobacillus gasseriwere identified only in several fecal samples from HC. Compared to HC, the composition ofBifidobacteriumspecies of T-CD varied, and it varied even more for U-CD. Forty-seven volatile organic compounds (VOCs) belonging to different chemical classes were identified using gas-chromatography mass spectrometry-solid-phase microextraction analysis. The median concentrations varied markedly for HC, T-CD, and U-CD. Overall, ther2values for VOC data for brothers and sisters were equal to or lower than those for unrelated HC and T-CD. This study shows the effect of CD pathology on the fecal microbiotas of children.

Quantitative Analysis of Volatile Organic Compounds by GC-MS

1977 ◽  
Vol 69 (11) ◽  
pp. 605-612 ◽  
Author(s):  
Robert D. Lingg ◽  
Robert G. Melton ◽  
Frederick C. Kopfler ◽  
W. Emile Coleman ◽  
Donald E. Mitchell
Keyword(s):  
Quantitative Analysis ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic
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