scholarly journals Label-free and real-time monitoring of human mesenchymal stem cell differentiation in 2D and 3D cell culture systems using impedance cell sensors

RSC Advances ◽  
2018 ◽  
Vol 8 (54) ◽  
pp. 31246-31254 ◽  
Author(s):  
Jun Ho Song ◽  
Sun-Mi Lee ◽  
Kyung-Hwa Yoo
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
3D Cell Culture ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Label Free ◽  
Culture Systems ◽  
Mesenchymal Stem Cell Differentiation ◽  
Cell Culture Systems ◽  
2D And 3D

3D impedance cell sensors are developed to monitor hMSC differentiation in label-free and real-time. Analyzing capacitance and conductance with these sensors shows that osteoblast and adipocyte lineages can be discriminated non-invasively in 3D cell culture systems.

scholarly journals Mammary gland 3D cell culture systems in farm animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurence Finot ◽  
Eric Chanat ◽  
Frederic Dessauge
Keyword(s):  
Cell Culture ◽  
Mammary Gland ◽  
Bacterial Infections ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Farm Animals ◽  
Culture Systems ◽  
Cell Culture Systems

AbstractIn vivo study of tissue or organ biology in mammals is very complex and progress is slowed by poor accessibility of samples and ethical concerns. Fortunately, however, advances in stem cell identification and culture have made it possible to derive in vitro 3D “tissues” called organoids, these three-dimensional structures partly or fully mimicking the in vivo functioning of organs. The mammary gland produces milk, the source of nutrition for newborn mammals. Milk is synthesized and secreted by the differentiated polarized mammary epithelial cells of the gland. Reconstructing in vitro a mammary-like structure mimicking the functional tissue represents a major challenge in mammary gland biology, especially for farm animals for which specific agronomic questions arise. This would greatly facilitate the study of mammary gland development, milk secretion processes and pathological effects of viral or bacterial infections at the cellular level, all with the objective of improving milk production at the animal level. With this aim, various 3D cell culture models have been developed such as mammospheres and, more recently, efforts to develop organoids in vitro have been considerable. Researchers are now starting to draw inspiration from other fields, such as bioengineering, to generate organoids that would be more physiologically relevant. In this chapter, we will discuss 3D cell culture systems as organoids and their relevance for agronomic research.

3D Cell Culture Systems for the Development of Neural Interfaces

2020 ◽  
pp. 201-236
Author(s):  
Omaer Syed ◽  
Chris Chapman ◽  
Catalina Vallejo-Giraldo ◽  
Martina Genta ◽  
Josef Goding ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Neural Interfaces ◽  
Culture Systems ◽  
Cell Culture Systems

scholarly journals Biophysically Defined and Cytocompatible Covalently Adaptable Networks as Viscoelastic 3D Cell Culture Systems

2013 ◽  
Vol 26 (6) ◽  
pp. 865-872 ◽  
Author(s):  
Daniel D. McKinnon ◽  
Dylan W. Domaille ◽  
Jennifer N. Cha ◽  
Kristi S. Anseth
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Cell Culture Systems

scholarly journals Alzheimer’s Disease: Current Perspectives and Advances in Physiological Modeling

2021 ◽  
Vol 8 (12) ◽  
pp. 211
Author(s):  
E. Josephine Boder ◽  
Ipsita A. Banerjee
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cell Culture ◽  
Stem Cell Differentiation ◽  
Experimental Manipulation ◽  
Model Systems ◽  
Culture Systems ◽  
Cell Culture Systems

Though Alzheimer’s disease (AD) is the most common cause of dementia, complete disease-modifying treatments are yet to be fully attained. Until recently, transgenic mice constituted most in vitro model systems of AD used for preclinical drug screening; however, these models have so far failed to adequately replicate the disease’s pathophysiology. However, the generation of humanized APOE4 mouse models has led to key discoveries. Recent advances in stem cell differentiation techniques and the development of induced pluripotent stem cells (iPSCs) have facilitated the development of novel in vitro devices. These “microphysiological” systems—in vitro human cell culture systems designed to replicate in vivo physiology—employ varying levels of biomimicry and engineering control. Spheroid-based organoids, 3D cell culture systems, and microfluidic devices or a combination of these have the potential to replicate AD pathophysiology and pathogenesis in vitro and thus serve as both tools for testing therapeutics and models for experimental manipulation.

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