scholarly journals Synthesis of DNA-guided silver nanoparticles on a graphene oxide surface: enhancing the antibacterial effect and the wound healing activity

RSC Advances ◽  
2018 ◽  
Vol 8 (49) ◽  
pp. 28238-28248 ◽  
Author(s):  
Chunyi Tong ◽  
Wei Zou ◽  
Weimin Ning ◽  
Jialong Fan ◽  
Li Li ◽  
...  

A simple two-step approach to synthesize a new nanocomposite by directly loading single-stranded DNA (ssDNA)-guided silver nanoparticles (AgNPs) on graphene oxide (ssDNA-AgNPs@GO) is developed.

RSC Advances ◽  
2019 ◽  
Vol 9 (67) ◽  
pp. 39434-39434
Author(s):  
Chunyi Tong ◽  
Wei Zou ◽  
Weimin Ning ◽  
Jialong Fan ◽  
Li Li ◽  
...  

Correction for ‘Synthesis of DNA-guided silver nanoparticles on a graphene oxide surface: enhancing the antibacterial effect and the wound healing activity’ by Chunyi Tong et al., RSC Adv., 2018, 8, 28238–28248.


Author(s):  
ANNAMALAI P ◽  
BALASHANMUGAM P ◽  
KALAICHELVAN PT

Objective: The present study was aimed to study at investigating the antibacterial potential of silver nanoparticles (AgNPs) coated cotton fabrics against different pathogens and also for their wound healing property using fibroblasts cells. Materials and Methods: The leaf extracts of Peltophorum pterocarpum were used for the synthesis of AgNPs and were characterizing using ultraviolet-visible spectrophotometer, transmission electron microscopy, energy dispersive X-ray (EDX) spectroscopy, dynamic light scattering analysis, and zeta potential measurement. The AgNPs were coated on cotton fabrics and tested for their antibacterial efficacy using agar well diffusion method. The wound healing property of synthesized AgNPs was tested using fibroblast 3T3 cells. Results: The plant extracts of P. pterocarpum were utilized for AgNPs. The optimum condition for synthesizing AgNPs was found to be 1 mg/ml plant concentration, 7 pH, 1 mM silver nitrate concatenation, and incubation temperature of 37°C. The shape of synthesized AgNPs was found to be spherical with an average size between 20 and 50 nm, and elemental silver peaks were confirmed by EDX spectrum. The cotton fabrics coated with AgNPs show good zone of inhibition against all the tested pathogens and the treated fabrics were also characterized using scanning electron microscope which reveals the presence of AgNPs on the fabrics. The scratch assay reveals that the AgNPs have good wound healing activity when tested against fibroblast 3T3. Conclusion: The present results conclude that the synthesized AgNPs have good stability with potent antimicrobial activity when coated with cotton fabrics. The AgNPs also found to have good activity significant wound healing activity when tested using fibroblast cells.


2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Palanivel Sangeetha ◽  
Natesan Geetha ◽  
Shivendra Vikram Sahi

The present study describes a rapid method for synthesis of metallic silver nanoparticles using callus (CAgNPs) and leaf extracts (LEAgNPs) ofCucumis sativusand evaluation of its wound healing activity in rat model. The prepared silver nanoparticles showed a peak at 350 nm corresponding to the surface plasmon resonance band. The FTIR spectroscopy measurements showed the presence of the possible biomolecules. X-ray diffraction analysis confirmed the crystalline structure of the synthesized silver nanoparticles. TEM images showed the size of the synthesized CAgNPs with diameter ranged from 21 nm to 23 nm with polygonal shape whereas, in the case of LEAgNPs, spherical shape was noticed with an average size between 11 nm and 19 nm. The EDX results indicated the chemical composition at specific locations on synthesized nanoparticles. Furthermore the topical application of ointment prepared using synthesized AgNPs was found to show enhanced wound healing activity in Wistar albino rat model. By the 21st day, the ointment base containing 5% (w/w) of silver nanoparticles showed 100% potential wound healing activity than the standard drug as well as control bases. Results strongly showed that the ointment base containing LEAgNPs was found to be very effective in wound repair mechanism in the experimental rats.


2021 ◽  
Author(s):  
Arturo Barjola ◽  
María Ángeles Tormo ◽  
Oscar Sahuquillo ◽  
Patricia Bernabé ◽  
José Manuel Pérez ◽  
...  

Abstract The strong bactericidal action of silver nanoparticles (AgNPs) is usually limited for their degree of aggregation. Deposition of AgNPs onto a graphene oxide (GO) surface to generate GO-Ag hybrids has been shown to be an effective method to control these aggregation problems. In this sense, a novel carboxylated graphene oxide-silver nanoparticle (GOCOOH-Ag) material has been synthesized and their antibacterial and biofilm formation inhibition have been studied.AgNPs decorating the GOCOOH surface achieved an average size of 6.74±0.25 nm, which was smaller than those of AgNPs deposited onto the GO surface. In addition, better distribution of AgNPs was obtained using carboxylated material. It is important to highlight the main role of the carboxylic groups in the nucleation and growth of the AgNPs that decorate the GO-based material surface.In vitro antibacterial activity and antibiofilm-forming action were tested against Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Both GO-Ag and GOCOOH-Ag reduced the bacterial growth, analyzed by time-kill curves. However, the minimum inhibitory concentration and the minimum bactericidal concentration of GOCOOH-Ag were lower than those of GO-Ag for all strains studied, indicating that GOCOOH-Ag has better antibacterial activity. In addition, both nanomaterials prevent biofilm-formation, with a higher reduction of biofilm mass and cell viability in the presence of GOCOOH-Ag. The carboxylation functionalization in GO-based materials can be applied to improve the bactericidal and antibiofilm-forming action of the AgNPs.


2019 ◽  
Vol 98 ◽  
pp. 1053-1063 ◽  
Author(s):  
Jinlong Shao ◽  
Bing Wang ◽  
Jinmeng Li ◽  
John A. Jansen ◽  
X. Frank Walboomers ◽  
...  

2014 ◽  
Vol 16 (9) ◽  
Author(s):  
Abhishek Kaler ◽  
Amit Kumar Mittal ◽  
Mahesh Katariya ◽  
Harshad Harde ◽  
Ashish Kumar Agrawal ◽  
...  

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