scholarly journals A nano-fibrous platform of copolymer patterned surfaces for controlled cell alignment

RSC Advances ◽  
2018 ◽  
Vol 8 (39) ◽  
pp. 21777-21785 ◽  
Author(s):  
Kai Zhang ◽  
Alexandra Arranja ◽  
Hongyu Chen ◽  
Serhii Mytnyk ◽  
Yiming Wang ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Cell Response ◽  
Flat Surface ◽  
Control Cell ◽  
Patterned Surfaces ◽  
Cell Alignment ◽  
Nano Structures ◽  
Cylindrical Micelles ◽  
Group Chemical ◽  
End Group

A method to transfer-print quenched, ultra-long copolymer cylindrical micelles to a flat surface and the use of these nano-structures to promote spontaneous cell alignment is proposed. Endless possibilities of corona end-group chemical modification provide a new tool to control cell response.

Nano Patterned Surfaces for Biomaterial Applications

2006 ◽  
Vol 53 ◽  
pp. 107-115 ◽  
Author(s):  
Nikolaj Gadegaard ◽  
Matthew J. Dalby ◽  
Elena Martines ◽  
Kris Seunarine ◽  
Mathis O. Riehle ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Flat Surface ◽  
Control Cell ◽  
Patterned Surfaces ◽  
Biological Applications ◽  
Adhesive Properties ◽  
Wetting Properties ◽  
The Past ◽  
Commercial Market ◽  
Patterned Materials

Bionanotechnology has seen much interest in the past few years. The development in new nanotechnologies and the transfer of such to biomedical applications has been received with large expectations. Here we will describe some of the most common techniques to prepare surfaces with nanometric sized features and how they have been applied to control cell behavior. The focus, however, will be on electron beam lithography and its use in biological applications. We will show that such highly ordered surfaces exhibit low adhesive properties for cells. Also, such topographies change the wetting properties to be either more hydrophilic or hydrophobic depending on the surface energy of the flat surface. Today, little research has found its way to the commercial market. This is mainly down to the ability to make large areas or large quantities of nano patterned materials. We will describe a few methods by which we think it would be possible to mass produce nano topographically patterned surfaces.

scholarly journals Primary cilia control cell alignment and patterning in bone development via ceramide-PKCζ-β-catenin signaling

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Jormay Lim ◽  
Xinhua Li ◽  
Xue Yuan ◽  
Shuting Yang ◽  
Lin Han ◽  
...  
Keyword(s):  
Primary Cilia ◽  
Bone Development ◽  
Control Cell ◽  
Cell Alignment

scholarly journals Alignment of Carbon Nanotubes: An Approach to Modulate Cell Orientation and Asymmetry

Nano LIFE ◽  
2014 ◽  
Vol 04 (01) ◽  
pp. 1450002 ◽  
Author(s):  
Qingsu Cheng ◽  
Greg M. Harris ◽  
Marc-Olivier Blais ◽  
Katy Rutledge ◽  
Ehsan Jabbarzadeh
Keyword(s):  
Stem Cells ◽  
Control Cell ◽  
Adhesion Formation ◽  
Embryonic Stem ◽  
Focal Adhesions ◽  
Biological Tissues ◽  
Spatiotemporal Pattern ◽  
Cell Alignment ◽  
Cell Orientation

Stem cells offer a promising tool in tissue engineering strategies, as their differentiated derivatives can be used to reconstruct most biological tissues. These approaches rely on controlling the biophysical cues that tune the ultimate fate of cells. In this context, significant effort has gone to parse out the role of conflicting matrix-elicited signals (e.g., topography and elasticity) in regulation of macroscopic characteristics of cells (e.g., shape and polarity). A critical hurdle, however, lies in our inability to recapitulate the nanoscale spatiotemporal pattern of these signals. The study presented in this manuscript took an initial step to overcome this challenge by developing a carbon nanotube (CNT)-based substrate for nanoresolution control of focal adhesion formation and cell alignment. The utility of this system was studied using human umbilical vascular endothelial cells (HUVECs) and human embryonic stem cells (hESCs) at a single cell level. Our results demonstrated the ability to control cell orientation by merely controlling the alignment of focal adhesions at a nanoscale size. Our long-term vision is to use these nanoengineered substrates to mimic cell orientation in earlier development and explore the role of polarity in asymmetric division and lineage specification of dividing cells.

Chemical modification of bioinspired superhydrophobic polystyrene surfaces to control cell attachment/proliferation

Soft Matter ◽  
2011 ◽  
Vol 7 (19) ◽  
pp. 8932 ◽  
Author(s):  
Sara M. Oliveira ◽  
Wenlong Song ◽  
Natália M. Alves ◽  
João F. Mano
Keyword(s):  
Chemical Modification ◽  
Cell Attachment ◽  
Control Cell

scholarly journals Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces

2005 ◽  
Vol 2 (2) ◽  
pp. 97-108 ◽  
Author(s):  
Jung Yul Lim ◽  
Joshua C Hansen ◽  
Christopher A Siedlecki ◽  
James Runt ◽  
Henry J Donahue
Keyword(s):  
Cell Response ◽  
Cell Function ◽  
Early Stage ◽  
Control Cell ◽  
Bone Cell ◽  
Cell Phenotype ◽  
Osteoblastic Cell ◽  
Bone Cell Differentiation ◽  
Biomaterial Interfaces ◽  
Cells Cultured

Nanoscale cell–substratum interactions are of significant interest in various biomedical applications. We investigated human foetal osteoblastic cell response to randomly distributed nanoisland topography with varying heights (11, 38 and 85 nm) produced by a polystyrene (PS)/polybromostyrene polymer-demixing technique. Cells displayed island-conforming lamellipodia spreading, and filopodia projections appeared to play a role in sensing the nanotopography. Cells cultured on 11 nm high islands displayed significantly enhanced cell spreading and larger cell dimensions than cells on larger nanoislands or flat PS control, on which cells often displayed a stellate shape. Development of signal transmitting structures such as focal adhesive vinculin protein and cytoskeletal actin stress fibres was more pronounced, as was their colocalization, in cells cultured on smaller nanoisland surfaces. Cell adhesion and proliferation were greater with decreasing island height. Alkaline phosphatase (AP) activity, an early stage marker of bone cell differentiation, also exhibited nanotopography dependence, i.e. higher AP activity on 11 nm islands compared with that on larger islands or flat PS. Therefore, randomly distributed island topography with varying nanoscale heights not only affect adhesion-related cell behaviour but also bone cell phenotype. Our results suggest that modulation of nanoscale topography may be exploited to control cell function at cell–biomaterial interfaces.

Geometric Control of Cell Alignment and Spreading within the Confinement of Antiadhesive Poly(Ethylene Glycol) Microstructures on Laser-Patterned Surfaces

2015 ◽  
Vol 1 (9) ◽  
pp. 747-752 ◽  
Author(s):  
Christine Strehmel ◽  
Heidi Perez-Hernandez ◽  
Zhenfang Zhang ◽  
Axel Löbus ◽  
Andrés F. Lasagni ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Geometric Control ◽  
Patterned Surfaces ◽  
Cell Alignment ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Micropatterning Decellularized ECM as a Bioactive Surface to Guide Cell Alignment, Proliferation, and Migration

2020 ◽  
Vol 7 (3) ◽  
pp. 102 ◽  
Author(s):  
Emily Cady ◽  
Jacob A. Orkwis ◽  
Rachel Weaver ◽  
Lia Conlin ◽  
Nicolas N. Madigan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Lithography ◽  
Cell Function ◽  
Control Cell ◽  
Three Dimensional ◽  
Protein Composition ◽  
Cell Alignment ◽  
Bioactive Component ◽  
And Migration ◽  
High Degree

Bioactive surfaces and materials have displayed great potential in a variety of tissue engineering applications but often struggle to completely emulate complex bodily systems. The extracellular matrix (ECM) is a crucial, bioactive component in all tissues and has recently been identified as a potential solution to be utilized in combination with biomaterials. In tissue engineering, the ECM can be utilized in a variety of applications by employing the biochemical and biomechanical cues that are crucial to regenerative processes. However, viable solutions for maintaining the dimensionality, spatial orientation, and protein composition of a naturally cell-secreted ECM remain challenging in tissue engineering. Therefore, this work used soft lithography to create micropatterned polydimethylsiloxane (PDMS) substrates of a three-dimensional nature to control cell adhesion and alignment. Cells aligned on the micropatterned PDMS, secreted and assembled an ECM, and were decellularized to produce an aligned matrix biomaterial. The cells seeded onto the decellularized, patterned ECM showed a high degree of alignment and migration along the patterns compared to controls. This work begins to lay the groundwork for elucidating the immense potential of a natural, cell-secreted ECM for directing cell function and offers further guidance for the incorporation of natural, bioactive components for emerging tissue engineering technologies.

scholarly journals Shape-Memory Nanofiber Meshes with Programmable Cell Orientation

Fibers ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 20 ◽  
Author(s):  
Eri Niiyama ◽  
Kanta Tanabe ◽  
Koichiro Uto ◽  
Akihiko Kikuchi ◽  
Mitsuhiro Ebara
Keyword(s):  
Mechanical Properties ◽  
Shape Memory ◽  
Rational Design ◽  
Control Cell ◽  
Hexamethylene Diisocyanate ◽  
Molar Ratio ◽  
Great Promise ◽  
Original Structure ◽  
Cell Alignment ◽  
Shape Memory Properties

In this work we report the rational design of temperature-responsive nanofiber meshes with shape-memory properties. Meshes were fabricated by electrospinning poly(ε-caprolactone) (PCL)-based polyurethane with varying ratios of soft (PCL diol) and hard [hexamethylene diisocyanate (HDI)/1,4-butanediol (BD)] segments. By altering the PCL diol:HDI:BD molar ratio both shape-memory properties and mechanical properties could be readily turned and modulated. Though mechanical properties improved by increasing the hard to soft segment ratio, optimal shape-memory properties were obtained using a PCL/HDI/BD molar ratio of 1:4:3. Microscopically, the original nanofibrous structure could be deformed into and maintained in a temporary shape and later recover its original structure upon reheating. Even when deformed by 400%, a recovery rate of >89% was observed. Implementation of these shape memory nanofiber meshes as cell culture platforms revealed the unique ability to alter human mesenchymal stem cell alignment and orientation. Due to their biocompatible nature, temperature-responsivity, and ability to control cell alignment, we believe that these meshes may demonstrate great promise as biomedical applications.

Ligand accessibility as means to control cell response to bioactive bilayer membranes

2000 ◽  
Vol 50 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Yoav Dori ◽  
Havazelet Bianco-Peled ◽  
Sushil K. Satija ◽  
Gregg B. Fields ◽  
James B. McCarthy ◽  
...  
Keyword(s):  
Cell Response ◽  
Control Cell ◽  
Bilayer Membranes

scholarly journals Molecular clutch drives cell response to surface viscosity

2018 ◽  
Vol 115 (6) ◽  
pp. 1192-1197 ◽  
Author(s):  
Mark Bennett ◽  
Marco Cantini ◽  
Julien Reboud ◽  
Jonathan M. Cooper ◽  
Pere Roca-Cusachs ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Cell Response ◽  
Loading Rate ◽  
Control Cell ◽  
Focal Adhesions ◽  
Surface Viscosity ◽  
Force Loading ◽  
Lower Viscosity ◽  
Order Of Magnitude ◽  
Myoblast Cells

Cell response to matrix rigidity has been explained by the mechanical properties of the actin-talin-integrin-fibronectin clutch. Here the molecular clutch model is extended to account for cell interactions with purely viscous surfaces (i.e., without an elastic component). Supported lipid bilayers present an idealized and controllable system through which to study this concept. Using lipids of different diffusion coefficients, the mobility (i.e., surface viscosity) of the presented ligands (in this case RGD) was altered by an order of magnitude. Cell size and cytoskeletal organization were proportional to viscosity. Furthermore, there was a higher number of focal adhesions and a higher phosphorylation of FAK on less-mobile (more-viscous) surfaces. Actin retrograde flow, an indicator of the force exerted on surfaces, was also seen to be faster on more mobile surfaces. This has consequential effects on downstream molecules; the mechanosensitive YAP protein localized to the nucleus more on less-mobile (more-viscous) surfaces and differentiation of myoblast cells was enhanced on higher viscosity. This behavior was explained within the framework of the molecular clutch model, with lower viscosity leading to a low force loading rate, preventing the exposure of mechanosensitive proteins, and with a higher viscosity causing a higher force loading rate exposing these sites, activating downstream pathways. Consequently, the understanding of how viscosity (regardless of matrix stiffness) influences cell response adds a further tool to engineer materials that control cell behavior.

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