scholarly journals iTRAQ-based quantitative proteomic analysis of dark-germinated soybeans in response to salt stress

RSC Advances ◽  
2018 ◽  
Vol 8 (32) ◽  
pp. 17905-17913 ◽  
Author(s):  
Yongqi Yin ◽  
Fei Qi ◽  
Lu Gao ◽  
Shengqi Rao ◽  
Zhenquan Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Quantitative Proteomic Analysis

iTRAQ-based quantitative proteomic analysis reveals the molecular mechanisms of dark-germinated soybeans in response to salt stress.

scholarly journals iTRAQ-Based Quantitative Proteomic Analysis of Cotton Roots and Leaves Reveals Pathways Associated with Salt Stress

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0148487 ◽  
Author(s):  
Tingting Chen ◽  
Lei Zhang ◽  
Haihong Shang ◽  
Shaodong Liu ◽  
Jun Peng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Quantitative Proteomic Analysis

scholarly journals Label-Free Quantitative Proteomic Analysis of the Global Response to Indole-3-Acetic Acid in Newly Isolated Pseudomonas sp. Strain LY1

2021 ◽  
Vol 12 ◽  
Author(s):  
Shuxue Zhao ◽  
Xi Chen ◽  
Qianshu Sun ◽  
Fei Wang ◽  
Chunhui Hu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Proteomic Analysis ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Degradation Mechanism ◽  
Label Free ◽  
Ros Scavenging ◽  
Protein Repair ◽  
Quantitative Proteomic Analysis ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid (IAA), known as a common plant hormone, is one of the most distributed indole derivatives in the environment, but the degradation mechanism and cellular response network to IAA degradation are still not very clear. The objective of this study was to elucidate the molecular mechanisms of IAA degradation at the protein level by a newly isolated strain Pseudomonas sp. LY1. Label-free quantitative proteomic analysis of strain LY1 cultivated with IAA or citrate/NH4Cl was applied. A total of 2,604 proteins were identified, and 227 proteins have differential abundances in the presence of IAA, including 97 highly abundant proteins and 130 less abundant proteins. Based on the proteomic analysis an IAA degrading (iad) gene cluster in strain LY1 containing IAA transformation genes (organized as iadHABICDEFG), genes of the β-ketoadipate pathway for catechol and protocatechuate degradation (catBCA and pcaABCDEF) were identified. The iadA, iadB, and iadE-disrupted mutants lost the ability to grow on IAA, which confirmed the role of the iad cluster in IAA degradation. Degradation intermediates were analyzed by HPLC, LC-MS, and GC-MS analysis. Proteomic analysis and identified products suggested that multiple degradation pathways existed in strain LY1. IAA was initially transformed to dioxindole-3-acetic acid, which was further transformed to isatin. Isatin was then transformed to isatinic acid or catechol. An in-depth data analysis suggested oxidative stress in strain LY1 during IAA degradation, and the abundance of a series of proteins was upregulated to respond to the stress, including reaction oxygen species (ROS) scavenging, protein repair, fatty acid synthesis, RNA protection, signal transduction, chemotaxis, and several membrane transporters. The findings firstly explained the adaptation mechanism of bacteria to IAA degradation.

iTRAQ-based quantitative proteomic analysis of wheat roots in response to salt stress

PROTEOMICS ◽  
2017 ◽  
Vol 17 (8) ◽  
pp. 1600265 ◽  
Author(s):  
Qiyan Jiang ◽  
Xiaojuan Li ◽  
Fengjuan Niu ◽  
Xianjun Sun ◽  
Zheng Hu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Wheat Roots ◽  
Quantitative Proteomic Analysis

Front Cover: iTRAQ-based quantitative proteomic analysis of wheat roots in response to salt stress

PROTEOMICS ◽  
2017 ◽  
Vol 17 (8) ◽  
pp. 1770060 ◽  
Author(s):  
Qiyan Jiang ◽  
Xiaojuan Li ◽  
Fengjuan Niu ◽  
Xianjun Sun ◽  
Zheng Hu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Wheat Roots ◽  
Front Cover ◽  
Quantitative Proteomic Analysis

scholarly journals Quantitative proteomic analysis of a genetically induced prostate inflammation mouse model via custom 4-plex DiLeu isobaric labeling

2019 ◽  
Vol 316 (6) ◽  
pp. F1236-F1243 ◽  
Author(s):  
Ling Hao ◽  
Samuel Thomas ◽  
Tyler Greer ◽  
Chad M. Vezina ◽  
Sagar Bajpai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Biomarker Discovery ◽  
Urinary Proteins ◽  
Quantitative Proteomic Analysis ◽  
Prostate Inflammation ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Isobaric Tags ◽  
Accurate Quantification

Inflammation is involved in many prostate pathologies including infection, benign prostatic hyperplasia, and prostate cancer. Preclinical models are critical to our understanding of disease mechanisms, yet few models are genetically tractable. Here, we present a comparative quantitative proteomic analysis of urine from mice with and without prostate-specific inflammation induced by conditional prostate epithelial IL-1β expression. Relative quantification and sample multiplexing was achieved using custom 4-plex N, N-dimethyl leucine (DiLeu) isobaric tags and nanoflow ultrahigh-performance liquid chromatography coupled to high-resolution tandem mass spectrometry. Each set of 4-plex DiLeu reagents allows four urine samples to be analyzed simultaneously, providing high-throughput and accurate quantification of urinary proteins. Proteins involved in the acute phase response, including haptoglobin, inter-α-trypsin inhibitor, and α1-antitrypsin 1-1, were differentially represented in the urine of mice with prostate inflammation. Mass spectrometry-based quantitative urinary proteomics represents a promising bioanalytical strategy for biomarker discovery and the elucidation of molecular mechanisms in urological research.

scholarly journals iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

2020 ◽  
Author(s):  
Jing Wang ◽  
Chengliang Liang ◽  
Sha Yang ◽  
Jingshuang Song ◽  
Xuefeng Li ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Tolerance ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Negative Impact ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
Heat Stress Response ◽  
Quantitative Proteomic Analysis

Abstract Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

scholarly journals iTRAQ-based quantitative proteomic analysis of salt stress in Spica Prunellae

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Zixiu Liu ◽  
Lisi Zou ◽  
Cuihua Chen ◽  
Hui Zhao ◽  
Ying Yan ◽  
...  
Keyword(s):  
Salt Stress ◽  
Proteomic Analysis ◽  
Quantitative Proteomic Analysis
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