scholarly journals 3β-Hydroxy-Δ5-steroidal congeners from a column fraction of Dendronephthya puetteri attenuate LPS-induced inflammatory responses in RAW 264.7 macrophages and zebrafish embryo model

RSC Advances ◽  
2018 ◽  
Vol 8 (33) ◽  
pp. 18626-18634 ◽  
Author(s):  
I. P. Shanura Fernando ◽  
Won Woo Lee ◽  
Thilina U. Jayawardena ◽  
Min-Cheol Kang ◽  
Yong-Seok Ann ◽  
...  
Keyword(s):  
Natural Product ◽  
Bioactive Compounds ◽  
Zebrafish Embryo ◽  
Inflammatory Responses ◽  
Marine Organisms ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Column Fraction ◽  
Action Mechanisms ◽  
Natural Product Research

Bioactive compounds from marine organisms and their action mechanisms have provided new insights into medicinal and natural product research.

Immunomodulatory Effect of Bifidobacterium, Lactobacillus, and Streptococcus Strains of Paraprobiotics in Lipopolysaccharide-Stimulated Inflammatory Responses in RAW-264.7 Macrophages

2021 ◽  
Vol 79 (1) ◽  
Author(s):  
Monique Michels ◽  
Gabriel Fernandes Alves Jesus ◽  
Ana Paula Lorenzen Voytena ◽  
Marina Rossetto ◽  
Fernanda Ramlov ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Immunomodulatory Effect ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages

scholarly journals Octominin Inhibits LPS-Induced Chemokine and Pro-inflammatory Cytokine Secretion from RAW 264.7 Macrophages via Blocking TLRs/NF-κB Signal Transduction

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 511 ◽  
Author(s):  
K. K. Asanka Sanjeewa ◽  
D. P. Nagahawatta ◽  
Hye-Won Yang ◽  
Jae Young Oh ◽  
Thilina U. Jayawardena ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Inflammatory Cytokine ◽  
Inflammatory Responses ◽  
Signal Transduction Pathway ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Potential Candidate ◽  
Transduction Pathway ◽  
Raw 264.7 Macrophages ◽  
In Silico Docking

Inflammation is a well-organized innate immune response that plays an important role during the pathogen attacks and mechanical injuries. The Toll-like receptors (TLR)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a major signal transduction pathway observed in RAW 264.7 macrophages during the inflammatory responses. Here, we investigated the anti-inflammatory effects of Octominin; a bio-active peptide developed from Octopus minor in RAW 264.7 macrophages in vitro. Octominin was found to inhibit lipopolysaccharides (LPS)-stimulated transcriptional activation of NF-κB in RAW 264.7 cells and dose-dependently decreased the mRNA expression levels of TLR4. Specifically, in silico docking results demonstrated that Octominin has a potential to inhibit TLR4 mediated inflammatory responses via blocking formation of TLR4/MD-2/LPS complex. We also demonstrated that Octominin could significantly inhibit LPS-induced secretion of pro-inflammatory cytokine (interleukin-β; IL-1β, IL-6, and tumor necrosis factor-α) and chemokines (CCL3, CCL4, CCL5, and CXCL10) from RAW 264.7 cells. Additionally, Octominin repressed the LPS-induced pro-inflammatory mediators including nitric oxide (NO), prostaglandin E2, inducible NO synthase, and cyclooxygenase 2 in macrophages. These results suggest that Octominin is a potential inhibitor of TLRs/NF-κB signal transduction pathway and is a potential candidate for the treatment of inflammatory diseases.

PKC412 (CGP41251) modulates the proliferation and lipopolysaccharide-induced inflammatory responses of RAW 264.7 macrophages

2007 ◽  
Vol 360 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Katsutoshi Miyatake ◽  
Hiroshi Inoue ◽  
Kahoko Hashimoto ◽  
Hiroshi Takaku ◽  
Yoichiro Takata ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages

Inhibition of inflammatory responses by FC-77, a perfluorochemical, in lipopolysaccharide-treated RAW 264.7 macrophages

2005 ◽  
Vol 31 (7) ◽  
pp. 977-984 ◽  
Author(s):  
Hung Chang ◽  
Fu-Chang Kuo ◽  
Yuan-Shu Lai ◽  
Tz-Chong Chou
Keyword(s):  
Inflammatory Responses ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages

Gamma-Linolenic Acid Inhibits Inflammatory Responses by Regulating NF-κB and AP-1 Activation in Lipopolysaccharide-Induced RAW 264.7 Macrophages

Inflammation ◽  
2009 ◽  
Vol 33 (1) ◽  
pp. 46-57 ◽  
Author(s):  
Cheng-Sue Chang ◽  
Hai-Lun Sun ◽  
Chong-Kuei Lii ◽  
Haw-Wen Chen ◽  
Pei-Yin Chen ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Inflammatory Responses ◽  
Raw 264.7 ◽  
Gamma Linolenic Acid ◽  
Raw 264.7 Macrophages

Euscaphic acid isolated from roots of Rosa rugosa inhibits LPS-induced inflammatory responses via TLR4-mediated NF-κB inactivation in RAW 264.7 macrophages

2012 ◽  
Vol 113 (6) ◽  
pp. 1936-1946 ◽  
Author(s):  
In-Tae Kim ◽  
Suran Ryu ◽  
Ji-Sun Shin ◽  
Jung-Hye Choi ◽  
Hee-Juhn Park ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Raw 264.7 ◽  
Rosa Rugosa ◽  
Raw 264.7 Macrophages

scholarly journals HO-1 Induction by Selaginella tamariscina Extract Inhibits Inflammatory Response in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
An-Na Won ◽  
Sun Ah Kim ◽  
Jung Yun Ahn ◽  
Jae-Hyun Han ◽  
Chang-Hyun Kim ◽  
...  
Keyword(s):  
Nuclear Translocation ◽  
Inflammatory Responses ◽  
Scientific Evidence ◽  
Radical Scavenging ◽  
Raw 264.7 Cells ◽  
Ros Generation ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Selaginella Tamariscina ◽  
Anti Inflammatory

Selaginella Herba is the dried, aerial part of Selaginella tamariscina (P.Beauv.) Spring and has been used to treat amenorrhea, abdominal pain, headaches, and hematuria in Korea. However, scientific evidence regarding the anti-inflammatory activity and action mechanism of Selaginella tamariscina is lacking. Thus, the present study was performed to investigate the anti-inflammatory and antioxidant activities of Selaginella tamariscina ethanol extract (STE) against lipopolysaccharide (LPS)-induced inflammatory responses and identify the molecular mechanism responsible. STE was prepared by heating in 70% ethanol and its quality was confirmed by HPLC. STE dose-dependently inhibited the productions of inflammatory mediators (NO and PGE2) and proinflammatory cytokines (IL-1β and IL-6) in LPS-stimulated RAW 264.7 cells. STE markedly suppressed the phosphorylations of MAPKs, IκB-α, and NF-κB and the nuclear translocation of NF-κB induced by LPS stimulation. In addition, STE exhibited good free radical scavenging activity and prevented ROS generation by LPS. STE also upregulated the expression of Nrf2 and HO-1 and promoted the nuclear translocation of Nrf2. Taken together, STE was found to have anti-inflammatory and antioxidant effects on RAW 264.7 macrophages and the mechanism appeared to involve the MAPK, NF-κB, and Nrf2/HO-1 signaling pathways. These results suggest that STE might be useful for preventing or treating inflammatory diseases and provide scientific evidence that supports the developments of herbal prescriptions or novel natural products.

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