scholarly journals An inflammatory memory and angiogenic self-assembling nanofiber hydrogel scaffold seeded with Akkermansia muciniphila to accelerate the healing of diabetic ischemic ulcers

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17357-17364 ◽  
Author(s):  
Panke Cheng ◽  
Liyang Yao ◽  
Xiaolong Chen ◽  
Xingxing Su ◽  
Xuejiao Su ◽  
...  
Keyword(s):  
Hydrogel Scaffold ◽  
Akkermansia Muciniphila ◽  
Ischemic Ulcer ◽  
Self Assembling ◽  
Novel Strategy ◽  
Ischemic Ulcers ◽  
Ulcer Treatment

In this research, a polypeptide skeleton was synthesized based on 3,4-dihydroxyphenylalanine (DOPA) and conjugated with inflammatory and angiogenesis peptides, combined with A. Muciniphila as a novel strategy for diabetic ischemic ulcer treatment.

scholarly journals Towards Developing Bioresponsive, Self-Assembled Peptide Materials: Dynamic Morphology and Fractal Nature of Nanostructured Matrices

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1539 ◽  
Author(s):  
Kyle Koss ◽  
Larry Unsworth
Keyword(s):  
Fine Structure ◽  
Focal Point ◽  
Peptide Delivery ◽  
Bioactive Molecules ◽  
Matrix Metalloproteinase 2 ◽  
Fractal Nature ◽  
Self Assembling ◽  
Delivery Platform ◽  
Peptide Materials ◽  
Novel Strategy

(Arginine-alanine-aspartic acid-alanine)4 ((RADA)4) nanoscaffolds are excellent candidates for use as peptide delivery vehicles: they are relatively easy to synthesize with custom bio-functionality, and assemble in situ to allow a focal point of release. This enables (RADA)4 to be utilized in multiple release strategies by embedding a variety of bioactive molecules in an all-in-one “construct”. One novel strategy focuses on the local, on-demand release of peptides triggered via proteolysis of tethered peptide sequences. However, the spatial-temporal morphology of self-assembling nanoscaffolds may greatly influence the ability of enzymes to both diffuse into as well as actively cleave substrates. Fine structure and its impact on the overall effect on peptide release is poorly understood. In addition, fractal networks observed in nanoscaffolds are linked to the fractal nature of diffusion in these systems. Therefore, matrix morphology and fractal dimension of virgin (RADA)4 and mixtures of (RADA)4 and matrix metalloproteinase 2 (MMP-2) cleavable substrate modified (RADA)4 were characterized over time. Sites of high (glycine-proline-glutamine-glycine+isoleucine-alanine-serine-glutamine (GPQG+IASQ), CP1) and low (glycine-proline-glutamine-glycine+proline-alanine-glycine-glutamine (GPQG+PAGQ), CP2) cleavage activity were chosen. Fine structure was visualized using transmission electron microscopy. After 2 h of incubation, nanofiber networks showed an established fractal nature; however, nanofibers continued to bundle in all cases as incubation times increased. It was observed that despite extensive nanofiber bundling after 24 h of incubation time, the CP1 and CP2 nanoscaffolds were susceptible to MMP-2 cleavage. The properties of these engineered nanoscaffolds characterized herein illustrate that they are an excellent candidate as an enzymatically initiated peptide delivery platform.

Hydrogel Scaffold Fabricated by 3D Printing & Ultrasonic Spraying

2014 ◽  
Vol 1027 ◽  
pp. 336-339
Author(s):  
Yu Li ◽  
Ping Yan Bian
Keyword(s):  
3D Printing ◽  
Ambient Temperature ◽  
Rheological Properties ◽  
Biological Material ◽  
Crosslinking Agent ◽  
Hydrogel Scaffold ◽  
Novel Strategy

This paper presents a novel strategy for fabricating hydrogel scaffold at ambient temperature by 3D printing supplemented with an ultrasonic spraying which involves crosslink reaction. The contradictory between the rheological properties and solidification of natural biological material for machinability and formability respectively at ambient temperature was solved. The gelled fraction and porosity were measured respectively to study the influence of crosslinking agent. The results showed that the two subjects appear a parabolic trend with the concentration of CaCl2 from 2% to 4%, but the tolerance of aperture decreases monotone.

scholarly journals Towards Developing Bioresponsive, Self-Assembled Peptide Materials: Dynamic Morphology and Fractal Nature of Nanostructured Matrices

2018 ◽  
Author(s):  
Kyle Koss ◽  
Larry Unsworth
Keyword(s):  
Fine Structure ◽  
Focal Point ◽  
Peptide Delivery ◽  
Bioactive Molecules ◽  
Matrix Metalloproteinase 2 ◽  
Fractal Nature ◽  
Self Assembling ◽  
Delivery Platform ◽  
Peptide Materials ◽  
Novel Strategy

(RADA)4 nanoscaffolds are excellent candidates for use as peptide delivery vehicles: they are relatively easy to synthesize with custom bio-functionality, and assemble in situ to allow a focal point of release. This enables (RADA)4 to be utilized in multiple release strategies by embedding a variety of bioactive molecules in an all-in-one ‘construct’. One novel strategy focuses on the local, on-demand release of peptides triggered via proteolysis of tethered peptide sequences. However, the spatial-temporal morphology of self-assembling nanoscaffolds may greatly influence the ability for enzymes to both diffuse into as well as actively cleave substrates. Fine structure and its impact on overall affect on peptide release is poorly understood. In addition, fractal networks observed in nanoscaffolds are linked to the fractal nature of diffusion in these systems. Therefore, matrix morphology and fractal dimension of virgin (RADA)4 and mixtures of (RADA)4 and matrix metalloproteinase 2 (MMP-2) cleavable substrate modified (RADA)4 were characterized over time. Sites of high (GPQG+IASQ, CP1) and low (GPQG+PAGQ, CP2) cleavage activity were chosen. Fine structure was visualized using established according to established methods. After 2 hrs of incubation, nanofiber networks showed an established fractal nature, however nanofibers continued to bundle in all cases as incubation times increased. It was observed that despite extensive nanofiber bundling after 24 hrs of incubation time, the CP1 and CP2 nanoscaffolds were susceptible to MMP-2 cleavage. The properties of these engineered nanoscaffolds characterized herein illustrate that they are an excellent candidate as an enzymatically initiated peptide delivery platform.

Controlled release of BSA-linked cisplatin through a PepGel self-assembling peptide nanofiber hydrogel scaffold

Amino Acids ◽  
2017 ◽  
Vol 49 (12) ◽  
pp. 2015-2021 ◽  
Author(s):  
Jun Liang ◽  
Gang Liu ◽  
Jing Wang ◽  
Xiuzhi Susan Sun
Keyword(s):  
Controlled Release ◽  
Hydrogel Scaffold ◽  
Self Assembling

scholarly journals Results of Infrapopliteal Endovascular Procedures Performed in Diabetic Patients with Critical Limb Ischemia and Tissue Loss from the Perspective of an Angiosome-Oriented Revascularization Strategy

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Francisco Acín ◽  
César Varela ◽  
Ignacio López de Maturana ◽  
Joaquín de Haro ◽  
Silvia Bleda ◽  
...  
Keyword(s):  
Limb Salvage ◽  
Ulcer Healing ◽  
Endovascular Procedures ◽  
Limb Ischemia ◽  
Tissue Loss ◽  
Diabetic Patients ◽  
Critical Ischemia ◽  
Ischemic Ulcer ◽  
Anatomical Classification ◽  
Ischemic Ulcers

Our aim was to describe our experience with infrapopliteal endovascular procedures performed in diabetic patients with ischemic ulcers and critical ischemia (CLI). A retrospective study of 101 procedures was performed. Our cohort was divided into groups according to the number of tibial vessels attempted and the number of patent tibial vessels achieved to the foot. An angiosome anatomical classification of ulcers were used to describe the local perfusion obtained after revascularization. Ischemic ulcer healing and limb salvage rates were measured. Ischemic ulcer healing at 12 months and limb salvage at 24 months was similar between a single revascularization and multiple revascularization attempts. The group in whom none patent tibial vessel to the foot was obtained presented lower healing and limb salvage rates. No differences were observed between obtaining a single patent tibial vessel versus more than one tibial vessel. Indirect revascularization of the ulcer through arterial-arterial connections provided similar results than those obtained after direct revascularization via its specific angiosome tibial artery. Our results suggest that, in CLI diabetic patients with ischemic ulcers that undergo infrapopliteal endovascular procedures, better results are expected if at least one patent vessel is obtained and flow is restored to the local ischemic area of the foot.

FORMATION OF REVERSED MICELLE NANORING BY A DESIGNED SURFACTANT-LIKE PEPTIDE

NANO ◽  
2012 ◽  
Vol 07 (04) ◽  
pp. 1250024 ◽  
Author(s):  
FENG QIU ◽  
YONGZHU CHEN ◽  
CHENGKANG TANG ◽  
YANRONG LU ◽  
JINGQIU CHENG ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Self Assembly ◽  
Molecular Model ◽  
Intermolecular Hydrogen Bond ◽  
Reversed Micelle ◽  
System A ◽  
Self Assembling ◽  
Potential Applications ◽  
Ft Ir ◽  
Novel Strategy

Designing self-assembling peptides as nanomaterials has been an attractive strategy in recent years, however, these peptides were usually studied in aqueous solutions for their self-assembling behaviors and applications. In this study, we have designed a surfactant-like peptide AGD with a wedge-like shape and studied its self-assembling behaviors in aqueous solution or nonpolar system. By analyzing the intermolecular hydrogen bond using FT-IR and characterizing the nanostructures with DLS, AFM and TEM, it was confirmed that AGD could not undergo self-assembly in aqueous solution while could self-assemble into well-ordered nanorings in nonpolar system. A molecular model has been proposed to explain how the nanorings were formed in the manner of reversed micelle. These results suggested a novel strategy to fabricate self-assembling peptide nanomaterials in nonpolar system, which could have potential applications in many fields.

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