scholarly journals Stereoselective C-terminal peptide elongation from Chan–Lam–Evans reaction generated isopropenyl esters

2019 ◽  
Vol 17 (8) ◽  
pp. 2103-2106 ◽  
Author(s):  
Luuk Steemers ◽  
Jan H. van Maarseveen
Keyword(s):  
Peptide Elongation

Peptide isoprenyl esters were made via the Cu-mediated Chan–Lam–Evans reaction and elongated via pyrazole/DMEP-catalysed aminolysis.

A Strategy for Thioamide Incorporation During Fmoc Solid-Phase Peptide Synthesis with Robust Stereochemical Integrity

2019 ◽  
Author(s):  
luis camacho III ◽  
Bryan J. Lampkin ◽  
Brett VanVeller
Keyword(s):  
Amino Acid ◽  
Functional Groups ◽  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Side Chains ◽  
Amino Acid Side Chains ◽  
Peptide Elongation

We describe a method to protect the sensitive stereochemistry of the thioamide—in analogy to the protection of the functional groups of amino acid side chains—in order to preserve the thioamide moiety during peptide elongation.<br>

scholarly journals Protein synthesis in the liver of rats injected with cholesteryl 14-methylhexadecanoate

1972 ◽  
Vol 129 (2) ◽  
pp. 367-372 ◽  
Author(s):  
E. Komárková ◽  
J. Hradec
Keyword(s):  
Protein Synthesis ◽  
Liver Tissue ◽  
Regulatory Mechanism ◽  
Relative Content ◽  
Time Intervals ◽  
Peptidyl Transfer ◽  
Significant Stimulation ◽  
Peptide Elongation

1. Rats were injected intraperitoneally with cholesteryl 14-methylhexadecanoate and killed after various intervals of time up to 3 days; ribosomes and cell sap were isolated from their liver tissue. These fractions were tested for their ability to participate in protein synthesis. 2. Protein synthesis in complete systems containing ribosomes, cell sap and all necessary cofactors was significantly enhanced at 12 and 72h after the injection and significantly inhibited at 24h. At early times after injection isolated ribosomes had a slightly enhanced ability to bind nRNA. Peptide-elongation processes (i.e. binding of aminoacyl-tRNA to ribosomes, peptidyl transfer and polyphenylalanine synthesis) showed significant stimulation or inhibition depending on the time after injection of the ester. 3. A correlation was found between the ability of cell sap to stimulate polyphenylalanine synthesis and the relative cholesteryl 14-methylhexadecanoate content in the postmicrosomal supernatant at different time-intervals after administration of the ester. No significant changes were found in its content in the whole liver tissue. 4. Since the injected ester has previously been shown to accumulate in some enzymic fractions, the changes in its relative content may represent a regulatory mechanism modulating the rate of protein synthesis.

scholarly journals CTP can replace GTP in reactions catalyzed by eukaryotic peptide elongation factor 1

FEBS Letters ◽  
1984 ◽  
Vol 177 (1) ◽  
pp. 112-114 ◽  
Author(s):  
Z. Tuháčková ◽  
M. Havránek ◽  
J. Hradec
Keyword(s):  
Elongation Factor ◽  
Peptide Elongation ◽  
Elongation Factor 1
Sign in / Sign up

Export Citation Format

Share Document