scholarly journals Protected 2′-deoxyribonucleoside triphosphate building blocks for the photocaging of epigenetic 5-(hydroxymethyl)cytosine in DNA

2018 ◽  
Vol 16 (30) ◽  
pp. 5427-5432 ◽  
Author(s):  
Soňa Boháčová ◽  
Zuzana Vaníková ◽  
Lenka Poštová Slavětínská ◽  
Michal Hocek
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks ◽  
Hydroxymethyl Cytosine ◽  
Deoxyribonucleoside Triphosphate

2′-Deoxyribonucleoside triphosphates containing 5-(hydroxymethyl)cytosine protected with photocleavable groups were prepared and studied as substrates for the enzymatic synthesis of DNA containing a photocaged epigenetic 5hmC base.

Enzymatic Synthesis of Neoglycopeptide Building Blocks

2000 ◽  
Vol 39 (2) ◽  
pp. 396-398 ◽  
Author(s):  
Delphine Ramos ◽  
Patrick Rollin ◽  
Werner Klaffke
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks

scholarly journals Chemo-Enzymatic Synthesis of Chiral Fluorine-Containing Building Blocks

2004 ◽  
Vol 58 (3) ◽  
pp. 104-107 ◽  
Author(s):  
Richard H. Blaauw ◽  
Denis R. Ijzendoorn ◽  
Jozef G.O. Cremers ◽  
Floris P.J.T. Rutjes ◽  
Quirinus B. Broxterman ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks

scholarly journals Enzymatic Synthesis of ω-Hydroxydodecanoic Acid By Employing a Cytochrome P450 from Limnobacter sp. 105 MED

Catalysts ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 54 ◽  
Author(s):  
Sung-Yeon Joo ◽  
Hee-Wang Yoo ◽  
Sharad Sarak ◽  
Byung-Gee Kim ◽  
Hyungdon Yun
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks ◽  
Dodecanoic Acid ◽  
The Novel ◽  
Efficient System ◽  
Redox Partners ◽  
Hydroxylated Fatty Acids ◽  
System 2 ◽  
Optimized Conditions

ω-Hydroxylated fatty acids are valuable and versatile building blocks for the production of various adhesives, lubricants, cosmetic intermediates, etc. The biosynthesis of ω-hydroxydodecanoic acid from vegetable oils is one of the important green pathways for their chemical-based synthesis. In the present study, the novel monooxygenase CYP153AL.m from Limnobacter sp. 105 MED was used for the whole-cell biotransformations. We constructed three-component system that was comprised of CYP153AL.m, putidaredoxin and putidaredoxin reductase from Pseudomonas putida. This in vivo study demonstrated that CYP153AL.m is a powerful catalyst for the biosynthesis of ω-hydroxydodecanoic acid. Under optimized conditions, the application of a solid-state powdered substrate rather than a substrate dissolved in DMSO significantly enhanced the overall reaction titer of the process. By employing this efficient system, 2 g/L of 12-hydroxydodecanoic acid (12-OHDDA) was produced from 4 g/L of its corresponding fatty acid, which was namely dodecanoic acid. Furthermore, the system was extended to produce 3.28 g/L of 12-OHDDA using 4 g/L of substrate by introducing native redox partners. These results demonstrate the utility of CYP153AL.m-catalyzed biotransformations in the industrial production of 12-OHDDA and other valuable building blocks.

(Chemo)enzymatic synthesis of dTDP-activated 2,6-dideoxysugars as building blocks of polyketide antibiotics

2001 ◽  
Vol 335 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Stefan Amann ◽  
Gerald Dräger ◽  
Carsten Rupprath ◽  
Andreas Kirschning ◽  
Lothar Elling
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks

scholarly journals Diversity-Oriented Enzymatic Synthesis of Cyclopropane Building Blocks

ACS Catalysis ◽  
2020 ◽  
Vol 10 (13) ◽  
pp. 7112-7116 ◽  
Author(s):  
Bruce J. Wittmann ◽  
Anders M. Knight ◽  
Julie L. Hofstra ◽  
Sarah E. Reisman ◽  
S. B. Jennifer Kan ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks

ChemInform Abstract: (Chemo)enzymatic Synthesis of dTDP-Activated 2,6-Dideoxysugars as Building Blocks of Polyketide Antibiotics.

ChemInform ◽  
2010 ◽  
Vol 33 (5) ◽  
pp. no-no
Author(s):  
Stefan Amann ◽  
Gerald Draeger ◽  
Carsten Rupprath ◽  
Andreas Kirschning ◽  
Lothar Elling
Keyword(s):  
Enzymatic Synthesis ◽  
Building Blocks
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