Nitric oxide release from a cucurbituril encapsulated NO-donor

2018 ◽  
Vol 16 (23) ◽  
pp. 4272-4278 ◽  
Author(s):  
A. Acuña ◽  
N. Basílio ◽  
M. Parajó ◽  
J. C. Mejuto ◽  
J. Pérez-Juste ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Chemical Stimulus ◽  
No Donor ◽  
Nitric Oxide Release

The denitrosation of a S-nitrosothiol derivative, nitrosomercaptopyridine (SNO+), can be inhibited by incorporation into the cucurbit[7]uril cavity. Owing to the reversible character of host : guest complexation, SNO+ can be expelled from the host cavity through the application of a chemical stimulus allowing controlled nitric oxide release.

scholarly journals Effect of nitric oxide release from NOR-3 on urea synthesis, viability and oxygen consumption of rat hepatocyte cultures

2007 ◽  
pp. 427-432
Author(s):  
R Chimenti ◽  
G Martino ◽  
S Mazzulla ◽  
S Sesti
Keyword(s):  
Nitric Oxide ◽  
Oxygen Consumption ◽  
Cell Viability ◽  
Urea Synthesis ◽  
Rat Hepatocyte ◽  
Trypan Blue Exclusion ◽  
No Donor ◽  
Nitric Oxide Release ◽  
Hepatocyte Cultures ◽  
Trypan Blue Exclusion Test

As nitric oxide is considered a mediator of liver oxidative metabolism during sepsis, we studied the effects of exogenous nitric oxide, produced by NO-donor, (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (NOR-3), on cell viability, urea biosynthesis and oxygen consumption in rat hepatocyte cultures. Nitric oxide release from NOR-3 was studied using 4,5-diaminofluorescein diacetate. Urea levels were measured by the spectrophotometric method. Cell viability was determined by the MTT test and trypan blue exclusion test, whereas oxygen consumption was measured by a polarographic technique. After 2 h treatment, NOR-3 induced an increase in the levels of nitric oxide. After 2 h of treatment and 24 h after the end of the treatment with NOR-3, both cell viability and urea synthesis were significantly reduced in comparison to the controls for NOR-3 concentrations equal to or greater than 50 microM. A reduction in oxygen consumption was observed in hepatocytes after 40 min treatment with 100 microM NOR-3, even if the cell viability was unchanged. Reduction of oxygen consumption is an early indicator of the metabolic alterations in hepatocytes exposed to nitric oxide. These findings suggest that nitric oxide accumulation acts on hepatocyte cultures inducing cell death and reduction of urea synthesis after 2 hours.

scholarly journals S-Nitroso-N-Acetyl-D-Penicillamine Modified Hyperbranched Polyamidoamine for High-Capacity Nitric Oxide Storage and Release

2020 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Sean P. Hopkins ◽  
Megan C. Frost
Keyword(s):  
Nitric Oxide ◽  
High Capacity ◽  
Implantable Devices ◽  
Resonance Spectroscopy ◽  
No Donor ◽  
Ionization Time ◽  
Nitric Oxide Release ◽  
Beneficial Effects ◽  
1H Nuclear Magnetic Resonance

Synthetic nitric oxide (NO)-donating materials have been shown to have many beneficial effects when incorporated into biomedical materials. When released in the correct dosage, NO has been shown to increase the biocompatibility of blood and tissue contacting materials, but materials are often limited in the amount of NO that can be administered over a period of time. To address this, hyperbranched polyamidoamine (HPAMAM) was modified with the S-nitrosothiol, S-nitroso-N-acetyl-D-penicillamine, and nitrosated to form a controlled, high-capacity NO-donating compound (SNAP-HPAMAM). This compound has the potential of modifying polymers to release NO over long periods of time by being blended into a variety of base polymers. Nitric oxide release was triggered by photoinitiation and through passive ion-mediated release seen under physiological conditions. A material that delivers the beneficial dose of NO over a long period of time would be able to greatly increase the biocompatibility of long-term implantable devices. Structural analysis of a generation 2 HPAMAM molecule was done through Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance spectroscopy (NMR), and matrix assisted laser desorption ionization, time of flight (MALDI-TOF) mass spectrometry. The NO capacity of the finalized generation 2 SNAP-HPAMAM compound was approximately 1.90 ± 0.116 µmol NO/mg. Quantification of the functional groups in the compound proved that an average of 6.40 ± 0.309 reactive primary amine sites were present compared to the 8 reactive sites on a perfectly synthesized generation 2 dendrimer. There is a substantial advantage of using the hyper-branched HPAMAM over purified dendrimers in terms of reduced labor and expense while still providing a high-capacity NO donor that can be blended into different polymer matrices.

scholarly journals Nitric Oxide Release from Antimicrobial Peptide Hydrogels for Wound Healing

Biomolecules ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 4 ◽  
Author(s):  
Joana Durão ◽  
Nuno Vale ◽  
Salomé Gomes ◽  
Paula Gomes ◽  
Cristina C. Barrias ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Antimicrobial Peptide ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Initial Increase ◽  
No Donor ◽  
Collagen Production ◽  
Nitric Oxide Release ◽  
Lysine Residues

Nitric oxide (NO) is an endogenously produced molecule that has been implicated in several wound healing mechanisms. Its topical delivery may improve healing in acute or chronic wounds. In this study an antimicrobial peptide was synthesized which self-assembled upon a pH shift, forming a hydrogel. The peptide was chemically functionalized to incorporate a NO-donor moiety on lysine residues. The extent of the reaction was measured by ninhydrin assay and the NO release rate was quantified via the Griess reaction method. The resulting compound was evaluated for its antimicrobial activity against Escherichia coli, and its effect on collagen production by fibroblasts was assessed. Time-kill curves point to an initial increase in bactericidal activity of the functionalized peptide, and collagen production by human dermal fibroblasts when incubated with the NO-functionalized peptide showed a dose-dependent increase in the presence of the NO donor within a range of 0–20 μM.

Characterization of GTMAC-Graft-Chitosan as a Bactericidal Agent for Nitric Oxide Release

2011 ◽  
Vol 236-238 ◽  
pp. 2967-2972 ◽  
Author(s):  
Yong Liu ◽  
Yan Sun ◽  
Peng Yang ◽  
Yao Xing Xu ◽  
Yan Li Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Molecular Weight ◽  
Inhibition Zone ◽  
Lower Molecular Weight ◽  
Water Control ◽  
No Donor ◽  
Nitric Oxide Release ◽  
E Coli ◽  
Molecular Weights ◽  
No Release

This work aims to prepare and characterize one kind of nitric oxide (NO)-releasing conjugation of quaternary ammonium salt to chitosan, as well as to evaluate the anti-bacterial properties of diazeniumdiolates and the changes in NO release properties. The newly synthesized diazeniumdiolates are obtained from glycidyl-trimethyl-ammonium chloride (GTMAC)-bearing chitosan derivatives (HTCC) with different molecular weights (280 and 670 KDa) and are used as NO donor species. An HTCC with high molecular weight (670 KDa) exhibits higher storage capacity for NO (up to 357.70 nmol NO/mg) than one with a low molecular weight (280 kDa). The NO release durations (7 h) observed for the HTCC diazeniumliolates with higher molecular weight (670 kDa) was slightly higher than that of HTCC diazeniumliolates with lower molecular weight (280 kDa). By determining the inhibition zone diameter, HTCC-NO with lower molecular weight (280 kDa) showed significantly higher inhibition capabilities againstE. colithan HTCC, crude chitosan, and water control.

Nitric oxide release from a biodegradable cysteine-based polyphosphazene

2016 ◽  
Vol 4 (11) ◽  
pp. 1987-1998 ◽  
Author(s):  
Alec Lutzke ◽  
Bella H. Neufeld ◽  
Megan J. Neufeld ◽  
Melissa M. Reynolds
Keyword(s):  
Nitric Oxide ◽  
Donor Group ◽  
No Donor ◽  
First Report ◽  
Nitric Oxide Release ◽  
No Release

First report of nitric oxide (NO) release from a biodegradable polyphosphazene containing theS-nitrosothiol NO donor group.

Estradiol-stimulated nitric oxide release in nervous tissue, vasculature, and gonads of the giant cockroachBlaberus craniifer

2004 ◽  
Vol 55 (1-4) ◽  
pp. 143-148 ◽  
Author(s):  
F. E. Nieto-Fernandez ◽  
F. Ianuzzi ◽  
Adriana Ruiz ◽  
Lilian Nodimele
Keyword(s):  
Nitric Oxide ◽  
Nervous Tissue ◽  
Nitric Oxide Release
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