Measurement of nanomechanical properties of DNA molecules by PeakForce atomic force microscopy based on DNA origami

Nanoscale ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 4707-4711 ◽  
Author(s):  
Lin Li ◽  
Ping Zhang ◽  
Jiang Li ◽  
Ying Wang ◽  
Yuhui Wei ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Origami ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force ◽  
Dna Strands

Characterization of the stiffness of thin DNA strands remains difficult.

scholarly journals Characterization of the nanomechanical properties of the fission yeast (Schizosaccharomyces pombe) cell surface by atomic force microscopy

2021 ◽  
Author(s):  
Ellie Gibbs ◽  
Justine Hsu ◽  
Kathryn Barth ◽  
John W. Goss
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Cell Surface ◽  
Fission Yeast ◽  
Surface Topography ◽  
Polarized Growth ◽  
Force Microscopy ◽  
Nanomechanical Properties ◽  
Atomic Force

ABSTRACTVariations in cell wall composition and biomechanical properties can contribute to the cellular plasticity required during complex processes such as polarized growth and elongation in microbial cells. This study utilizes atomic force microscopy (AFM) to map the cell surface topography of fission yeast, Schizosaccharomyces pombe, at regions of active polarized growth and to characterize the biophysical properties within these regions under physiological, hydrated conditions. High-resolution images acquired from AFM topographic scanning reveal decreased surface roughness at actively growing cell poles. Force extension curves acquired by nanoindentation probing with AFM cantilever tips under low applied force revealed increased cell wall elasticity and decreased cellular stiffness (cellular spring constant) at cell poles (17 ± 4 mN/m) relative to the main body of the cell that is not undergoing growth and expansion (44 ± 10 mN/m). These findings suggest that the increased elasticity and decreased stiffness at regions undergoing polarized growth at fission yeast cell poles provide the plasticity necessary for cellular extension. This is the first direct biophysical characterization of the S. pombe cell surface by AFM, and it provides a foundation for future investigation of how the surface topography and local nanomechanical properties vary during different cellular processes.

Stereometric characterization of Dinizia excelsa Ducke wood from Amazon rainforest using atomic force microscopy

2021 ◽  
Author(s):  
Willian Silva Conceição ◽  
Ştefan Ţălu ◽  
Robert Saraiva Matos ◽  
Glenda Quaresma Ramos ◽  
Fidel Guereiro Zayas ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Amazon Rainforest ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dinizia Excelsa
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