Silvered conical-bottom 96-well plates: enhanced low volume detection and the metal-enhanced fluorescence volume/ratio effect

Nanoscale ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 4337-4344 ◽  
Author(s):  
Rachael Knoblauch ◽  
Chris D. Geddes
Keyword(s):  
Volume Ratio ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Ratio Effect ◽  
Low Volume ◽  
Fluorescence Volume ◽  
Conical Bottom

Silver conical-bottom 96-well plate platforms permit detection of fluorophores with microliter volumes, and the calculation of corrected metal-enhanced fluorescence values.

Metal-enhanced fluorescence from tin nanostructured surfaces

2010 ◽  
Vol 107 (2) ◽  
pp. 024302 ◽  
Author(s):  
Yongxia Zhang ◽  
Anatoliy Dragan ◽  
Chris D. Geddes
Keyword(s):  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Nanostructured Surfaces

UV-Trained and Metal-Enhanced Fluorescence of Biliverdin and Biliverdin Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Parinaz Fathi ◽  
Ayman Roslend ◽  
Kritika Mehta ◽  
Parikshit Moitra ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Biomedical Imaging ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

Increasing the fluorescence quantum yield of fluorophores is of great interest for in vitro and in vivo biomedical imaging applications. At the same time, photobleaching and photodegradation resulting from continuous...

Metal-enhanced fluorescence of carbon dots adsorbed Ag@SiO2 core-shell nanoparticles

RSC Advances ◽  
2012 ◽  
Vol 2 (5) ◽  
pp. 1765 ◽  
Author(s):  
Cuiyan Li ◽  
Yihua Zhu ◽  
Xiaoqing Zhang ◽  
Xiaoling Yang ◽  
Chunzhong Li
Keyword(s):  
Carbon Dots ◽  
Core Shell ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Shell Nanoparticles ◽  
Core Shell Nanoparticles

scholarly journals Single Molecule Techniques Can Distinguish the Photophysical Processes Governing Metal-Enhanced Fluorescence

2022 ◽  
Author(s):  
Stefania Impellizzeri ◽  
Gregory J, Hodgson ◽  
Nicholas P. Dogantzis
Keyword(s):  
Single Molecule ◽  
Organic Molecules ◽  
Near Field ◽  
Comprehensive Understanding ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Maximum Enhancement ◽  
Molecular Systems ◽  
Photophysical Processes ◽  
Underlying Processes

<p>Plasmonic metal nanoparticles can impact the behaviour of organic molecules in a number of ways, including enhancing or quenching fluorescence. Only through a comprehensive understanding of the fundamental photophysical processes regulating nano-molecular interactions can these effects be controlled, and exploited to the fullest extent possible. Metal-enhanced fluorescence (MEF) is governed by two underlying processes, increased rate of fluorophore excitation and increased fluorophore emission, the balance between which has implications for optimizing hybrid nanoparticle-molecular systems for various applications. We report groundbreaking work on the use of single molecule fluorescence microscopy to distinguish between the two mechanistic components of MEF, in a model system consisting of two analogous boron dipyrromethene (BODIPY) fluorophores and triangular silver nanoparticles (AgNP). We demonstrate that the increased excitation MEF mechanism occurs to approximately the same extent for both dyes, but that the BODIPY with the higher quantum yield of fluorescence experiences a greater degree of MEF via the increased fluorophore emission mechanism, and higher overall enhancement, as a result of its superior ability to undergo near-field interactions with AgNP. We foresee that this knowledge and methodology will be used to tailor MEF to meet the needs of different applications, such as those requiring maximum enhancement of fluorescence intensity or instead prioritizing excited-state photochemistry. </p>

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