Controllable and reversible DNA translocation through a single-layer molybdenum disulfide nanopore

Nanoscale ◽  
2018 ◽  
Vol 10 (41) ◽  
pp. 19450-19458 ◽  
Author(s):  
Wei Si ◽  
Yin Zhang ◽  
Jingjie Sha ◽  
Yunfei Chen
Keyword(s):  
Dna Sequencing ◽  
Molybdenum Disulfide ◽  
High Throughput ◽  
Low Cost ◽  
Single Layer ◽  
Dna Translocation ◽  
Nanopore Dna Sequencing

A challenge that remains to be solved in the high-throughput and low-cost nanopore DNA sequencing is that DNA translocates through the nanopore too quickly to be sequenced with enough accuracy.

scholarly journals Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening

BioTechniques ◽  
2019 ◽  
Vol 67 (3) ◽  
pp. 118-122 ◽  
Author(s):  
Fleur Hammet ◽  
Khalid Mahmood ◽  
Thomas R Green ◽  
Tu Nguyen-Dumont ◽  
Melissa C Southey ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Dna Sequencing ◽  
Multiplex Pcr ◽  
High Throughput ◽  
Genetic Screening ◽  
Low Cost ◽  
Substantial Reduction ◽  
Targeted Sequencing ◽  
Robust Approach ◽  
Panel Size

We have previously reported Hi-Plex, a multiplex PCR methodology for building targeted DNA sequencing libraries that offers a low-cost protocol compatible with high-throughput processing. Here, we detail an improved protocol, Hi-Plex2, that more effectively enables the robust construction of small-to-medium panel-size libraries while maintaining low cost, simplicity and accuracy benefits of the Hi-Plex platform. Hi-Plex2 was applied to three panels, comprising 291, 740 and 1193 amplicons, targeting genes associated with risk for breast and/or colon cancer. We show substantial reduction of off-target amplification to enable library construction for small-to-medium-sized design panels not possible using the previous Hi-Plex chemistry.

scholarly journals Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array

2016 ◽  
Vol 113 (44) ◽  
pp. E6749-E6756 ◽  
Author(s):  
P. Benjamin Stranges ◽  
Mirkó Palla ◽  
Sergey Kalachikov ◽  
Jeff Nivala ◽  
Michael Dorwart ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Dna Synthesis ◽  
Lipid Bilayers ◽  
High Throughput ◽  
Single Molecule ◽  
Low Cost ◽  
Electrode Array ◽  
Oxide Semiconductor ◽  
Sequencing Platform ◽  
Sequencing By Synthesis

Scalable, high-throughput DNA sequencing is a prerequisite for precision medicine and biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis (Nanopore-SBS) approach, which used a set of nucleotides with polymer tags that allow discrimination of the nucleotides in a biological nanopore. Here, we designed and covalently coupled a DNA polymerase to an α-hemolysin (αHL) heptamer using the SpyCatcher/SpyTag conjugation approach. These porin–polymerase conjugates were inserted into lipid bilayers on a complementary metal oxide semiconductor (CMOS)-based electrode array for high-throughput electrical recording of DNA synthesis. The designed nanopore construct successfully detected the capture of tagged nucleotides complementary to a DNA base on a provided template. We measured over 200 tagged-nucleotide signals for each of the four bases and developed a classification method to uniquely distinguish them from each other and background signals. The probability of falsely identifying a background event as a true capture event was less than 1.2%. In the presence of all four tagged nucleotides, we observed sequential additions in real time during polymerase-catalyzed DNA synthesis. Single-polymerase coupling to a nanopore, in combination with the Nanopore-SBS approach, can provide the foundation for a low-cost, single-molecule, electronic DNA-sequencing platform.

scholarly journals An Automated Sample Preparation System for Large-Scale DNA Sequencing

1999 ◽  
Vol 9 (5) ◽  
pp. 457-462 ◽  
Author(s):  
Andre Marziali ◽  
Thomas D. Willis ◽  
Nancy A. Federspiel ◽  
Ronald W. Davis
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
High Throughput ◽  
Large Scale ◽  
Low Cost ◽  
High Quality ◽  
Dna Templates ◽  
Sequencing Technologies ◽  
Front End ◽  
Automated Sample Preparation

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.

Bamboo Waste-based Bio-composite Substance: An application for Low-cost Construction Materials

2020 ◽  
Vol 4 (1) ◽  
pp. 41-48
Author(s):  
Teodoro Astorga Amatosa ◽  
Michael E. Loretero
Keyword(s):  
Composite Materials ◽  
Sea Water ◽  
Raw Materials ◽  
Low Cost ◽  
Construction Materials ◽  
Single Layer ◽  
High Strength ◽  
Green Technology ◽  
Experimental Medium ◽  
Natural Treatment

Bamboo is a lightweight and high-strength raw materials that encouraged researchers to investigate and explore, especially in the field of biocomposite and declared as one of the green-technology on the environment as fully accountable as eco-products. This research was to assess the technical feasibility of making single-layer experimental Medium-Density Particleboard panels from the bamboo waste of a three-year-old (Dendrocalamus asper). Waste materials were performed to produce composite materials using epoxy resin (C21H25C105) from a natural treatment by soaking with an average of pH 7.6 level of sea-water. Three different types of MDP produced, i.e., bamboo waste strip MDP (SMDP), bamboo waste chips MDP (CMDP) and bamboo waste mixed strip-chips MDP (MMDP) by following the same process. The experimental panels tested for their physical-mechanical properties according to the procedures defined by ASTM D1037-12. Conclusively, even the present study shows properties of MDP with higher and comparable to other composite materials; further research must be given better attention as potential substitute to be used as hardwood materials, especially in the production, design, and construction usage.

A Novel Wideband transition from Substrate Integrated Waveguide to Rectangular Waveguide

2020 ◽  
Vol 10 ◽  
Author(s):  
Keyur Mahant ◽  
Hiren Mewada ◽  
Amit Patel ◽  
Alpesh Vala ◽  
Jitendra Chaudhari
Keyword(s):  
Rectangular Waveguide ◽  
Printed Circuit Board ◽  
Low Cost ◽  
Single Layer ◽  
Circuit Board ◽  
Substrate Integrated Waveguide ◽  
Ka Band ◽  
Better Than ◽  
Millimeter Wave Circuits ◽  
Wideband Transition

Aim: In this article, wideband substrate integrated waveguide (SIW) and rectangular waveguide (RWG) transition operating in Ka-band is proposed Objective: In this article, wideband substrate integrated waveguide (SIW) and rectangular waveguide (RWG) transition operating in Ka-band is proposed. Method: Coupling patch etched on the SIW cavity to couple the electromagnetic energy from SIW to RWG. Moreover, metasurface is introduced into the radiating patch to enhance bandwidth. To verify the functionality of the proposed structure back to back transition is designed and fabricated on a single layer substrate using standard printed circuit board (PCB) fabrication technology. Results: Measured results matches with the simulation results, measured insertion loss is less than 1.2 dB and return loss is better than 3 dB for the frequency range of 28.8 to 36.3 GHz. By fabricating transition with 35 SRRs bandwidth of the proposed transition can be improved. Conclusion: The proposed transition has advantages like compact in size, easy to fabricate, low cost and wide bandwidth. Proposed structure is a good candidate for millimeter wave circuits and systems.

scholarly journals Organ-specific, multimodal, wireless optoelectronics for high-throughput phenotyping of peripheral neural pathways

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Woo Seok Kim ◽  
Sungcheol Hong ◽  
Milenka Gamero ◽  
Vivekanand Jeevakumar ◽  
Clay M. Smithhart ◽  
...  
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Optoelectronic Device ◽  
Antenna System ◽  
Neural Pathways ◽  
Organ Specific ◽  
Coil Antenna ◽  
Sensory Fibers

AbstractThe vagus nerve supports diverse autonomic functions and behaviors important for health and survival. To understand how specific components of the vagus contribute to behaviors and long-term physiological effects, it is critical to modulate their activity with anatomical specificity in awake, freely behaving conditions using reliable methods. Here, we introduce an organ-specific scalable, multimodal, wireless optoelectronic device for precise and chronic optogenetic manipulations in vivo. When combined with an advanced, coil-antenna system and a multiplexing strategy for powering 8 individual homecages using a single RF transmitter, the proposed wireless telemetry enables low cost, high-throughput, and precise functional mapping of peripheral neural circuits, including long-term behavioral and physiological measurements. Deployment of these technologies reveals an unexpected role for stomach, non-stretch vagal sensory fibers in suppressing appetite and demonstrates the durability of the miniature wireless device inside harsh gastric conditions.

scholarly journals A single layer deposition of Li-doped mesoporous TiO2 beads for low-cost and efficient dye-sensitized solar cells

2021 ◽  
Vol 45 (5) ◽  
pp. 2470-2477
Author(s):  
P. Golvari ◽  
E. Nouri ◽  
N. Mohsenzadegan ◽  
M. R. Mohammadi ◽  
S. O. Martinez-Chapa
Keyword(s):  
Light Scattering ◽  
Low Cost ◽  
Dye Sensitized Solar Cells ◽  
Single Layer ◽  
Cost Effective ◽  
Mesoporous Tio2 ◽  
Trap States ◽  
Dye Sensitized ◽  
Layer Deposition ◽  
Sensitized Solar Cells

Cost-effective DSCs with superior electronic properties are gained by a reduction in electronic trap states and outstanding light scattering and harvesting.

scholarly journals Single-Layer Line-Fed Broadband Microstrip Patch Antenna on Thin Substrates

Electronics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 37
Author(s):  
Roberto Vincenti Gatti ◽  
Riccardo Rossi ◽  
Marco Dionigi
Keyword(s):  
Patch Antenna ◽  
State Of The Art ◽  
Low Cost ◽  
Single Layer ◽  
Microstrip Antennas ◽  
Microstrip Patch Antenna ◽  
Layer Line ◽  
Limited Bandwidth ◽  
Microstrip Patch ◽  
Effective Design

In this work, the issue of limited bandwidth typical of microstrip antennas realized on a single thin substrate is addressed. A simple yet effective design approach is proposed based on the combination of traditional single-resonance patch geometries. Two novel shaped microstrip patch antenna elements with an inset feed are presented. Despite being printed on a single-layer substrate with reduced thickness, both radiators are characterized by a broadband behavior. The antennas are prototyped with a low-cost and fast manufacturing process, and measured results validate the simulations. State-of-the-art performance is obtained when compared to the existing literature, with measured fractional bandwidths of 3.71% and 6.12% around 10 GHz on a 0.508-mm-thick Teflon-based substrate. The small feeding line width could be an appealing feature whenever such radiating elements are to be used in array configurations.

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