scholarly journals Systemic siRNA delivery to tumors by cell-penetrating α-helical polypeptide-based metastable nanoparticles

Nanoscale ◽  
2018 ◽  
Vol 10 (32) ◽  
pp. 15339-15349 ◽  
Author(s):  
Yang Liu ◽  
Ziyuan Song ◽  
Nan Zheng ◽  
Kenya Nagasaka ◽  
Lichen Yin ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Targeting ◽  
Sirna Delivery ◽  
Tumor Penetration ◽  
Cell Internalization ◽  
Cell Penetrating

Metastable nanoparticles capable of tumor targeting, tumor penetration, and selective tumor cell internalization were developed based on membrane penetrating, helical polypeptide PVBLG-8 and anionic PLG, for the efficient encapsulation and delivery of EGFR siRNA.

Peptide-based combination nanoformulations for cancer therapy

Nanomedicine ◽  
2020 ◽  
Vol 15 (22) ◽  
pp. 2201-2217
Author(s):  
Neha Mehrotra ◽  
Surender Kharbanda ◽  
Harpal Singh
Keyword(s):  
Cancer Therapy ◽  
Hormone Therapy ◽  
Tumor Targeting ◽  
High Specificity ◽  
Peptide Vaccines ◽  
Tumor Penetration ◽  
Anticancer Peptides ◽  
Cell Penetrating ◽  
Therapeutic Research ◽  
Intracellular Targets

Research in cancer therapy is moving towards the use of biomolecules in combination with conventional approaches for improved disease outcome. Among the biomolecules explored, peptides are strong contenders due to their small size, high specificity, low systemic toxicity and wide inter/intracellular targets. The use of nanoformulations for such combination approaches can lead to further improvement in efficacy by reducing off-target cytotoxicity, increasing circulation time, tumor penetration and accumulation. This review focuses on nanodelivery systems for peptide-based combinations with chemo, immuno, radiation and hormone therapy. It gives an overview of the latest therapeutic research being conducted using combination nanoformulations with anticancer peptides, cell penetrating/tumor targeting peptides, peptide nanocarriers, peptidomimetics, peptide-based hormones and peptide vaccines. The challenges hindering clinical translation are also discussed.

scholarly journals Cationic Peptide-Modified Gold Nanostars as Efficient Delivery Platform for RNA Interference Antitumor Therapy

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3764
Author(s):  
Si Chen ◽  
Jiguang Li ◽  
Xiaoyu Ma ◽  
Fan Liu ◽  
Guoping Yan
Keyword(s):  
Tumor Cell ◽  
Tumor Targeting ◽  
Target Genes ◽  
Tumor Therapy ◽  
Sirna Delivery ◽  
Peptide Sequence ◽  
Antitumor Therapy ◽  
Gold Nanostars ◽  
Targeting Peptide ◽  
Tumor Targeting Peptide

siRNA interference therapy can silence tumor cell target genes and specifically regulate tumor cell behavior and function, which is an effective antitumor therapy. However, in somatic circulation, naked siRNAs are not only susceptible to degrade, but it is also difficult to realize the tumor cells’ internalization. Therefore, novel siRNA delivery vectors that could promote efficacy need to be developed urgently. Here, we designed high-surface gold nanostars (GNS-P) which are decorated with cationic tumor-targeting peptide as an efficient and functional siRNA delivery nanoplatform for tumor therapy. The positively charged amino acid sequence and huge surface area enabled the vector to load a large amount of siRNA, while the tumor-targeting peptide sequence and nano size enabled it to rapidly and precisely target the tumor regions for fast and effective siRNA delivery. This tumor-targeting nanoplatform, GNS-P, displayed good biocompatibility, low toxicity and an extraordinary tumor accumulation capability.

Biocompatible, chimeric peptide-condensed supramolecular nanoparticles for tumor cell-specific siRNA delivery and gene silencing

2014 ◽  
Vol 50 (58) ◽  
pp. 7806-7809 ◽  
Author(s):  
Hangxiang Wang ◽  
Wei Chen ◽  
Haiyang Xie ◽  
Xuyong Wei ◽  
Shengyong Yin ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Gene Silencing ◽  
Delivery System ◽  
Self Assembly ◽  
Sirna Delivery ◽  
Single Step ◽  
Chimeric Peptide ◽  
Supramolecular Nanoparticles ◽  
Sirna Delivery System

A practical and tumor cell-specific siRNA delivery system was developedviasingle-step self-assembly of an arginine-rich chimeric peptide with siRNA.

scholarly journals Tumor targeting of a cell penetrating peptide by fusing with a pH-sensitive histidine-glutamate co-oligopeptide

Biomaterials ◽  
2014 ◽  
Vol 35 (13) ◽  
pp. 4082-4087 ◽  
Author(s):  
Likun Fei ◽  
Li-Peng Yap ◽  
Peter S. Conti ◽  
Wei-Chiang Shen ◽  
Jennica L. Zaro
Keyword(s):  
Tumor Targeting ◽  
Ph Sensitive ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell

scholarly journals β-Lactamase Tools for Establishing Cell Internalization and Cytosolic Delivery of Cell Penetrating Peptides

Biomolecules ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 51 ◽  
Author(s):  
Shane Stone ◽  
Tatjana Heinrich ◽  
Suzy Juraja ◽  
Jiulia Satiaputra ◽  
Clinton Hall ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Cell Penetrating Peptides ◽  
Stable Cell Line ◽  
Intracellular Space ◽  
Biologically Relevant ◽  
Cell Internalization ◽  
Cytosolic Delivery ◽  
Cell Penetrating ◽  
Split Protein

The ability of cell penetrating peptides (CPPs) to deliver biologically relevant cargos into cells is becoming more important as targets in the intracellular space continue to be explored. We have developed two assays based on CPP-dependent, intracellular delivery of TEM-1 β-lactamase enzyme, a functional biological molecule comparable in size to many protein therapeutics. The first assay focuses on the delivery of full-length β-lactamase to evaluate the internalization potential of a CPP sequence. The second assay uses a split-protein system where one component of β-lactamase is constitutively expressed in the cytoplasm of a stable cell line and the other component is delivered by a CPP. The delivery of a split β-lactamase component evaluates the cytosolic delivery capacity of a CPP. We demonstrate that these assays are rapid, flexible and have potential for use with any cell type and CPP sequence. Both assays are validated using canonical and novel CPPs, with limits of detection from <500 nM to 1 µM. Together, the β-lactamase assays provide compatible tools for functional characterization of CPP activity and the delivery of biological cargos into cells.

Cell-penetrating peptides for siRNA delivery to glioblastomas

Peptides ◽  
2018 ◽  
Vol 104 ◽  
pp. 62-69 ◽  
Author(s):  
Artita Srimanee ◽  
Maria Arvanitidou ◽  
Kumjee Kim ◽  
Mattias Hällbrink ◽  
Ülo Langel
Keyword(s):  
Sirna Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating
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