scholarly journals Engineering strategies for rational polyketide synthase design

2018 ◽  
Vol 35 (10) ◽  
pp. 1070-1081 ◽  
Author(s):  
Maja Klaus ◽  
Martin Grininger
Keyword(s):  
Protein Interactions ◽  
Assembly Line ◽  
Polyketide Synthase ◽  
Polyketide Synthases ◽  
Protein Protein Interactions ◽  
Important Protein ◽  
Line P

In this review, we highlight strategies in engineering polyketide synthases (PKSs). We focus on important protein–protein interactions that constitute an intact PKS assembly line.

scholarly journals Protein-Protein Interactions, Not Substrate Recognition, Dominate the Turnover of Chimeric Assembly Line Polyketide Synthases

2016 ◽  
Vol 291 (31) ◽  
pp. 16404-16415 ◽  
Author(s):  
Maja Klaus ◽  
Matthew P. Ostrowski ◽  
Jonas Austerjost ◽  
Thomas Robbins ◽  
Brian Lowry ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Assembly Line ◽  
Substrate Recognition ◽  
Polyketide Synthases ◽  
Protein Protein Interactions

scholarly journals Probing the Selectivity and Protein⋅Protein Interactions of a Nonreducing Fungal Polyketide Synthase Using Mechanism-Based Crosslinkers

2014 ◽  
Vol 21 (7) ◽  
pp. 913
Author(s):  
Joel Bruegger ◽  
Robert W. Haushalter ◽  
Anna L. Vagstad ◽  
Joris Beld ◽  
Gaurav Shakya ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Polyketide Synthase ◽  
Protein Protein Interactions

Computational investigation of the α2β1 integrin–collagen triple helix complex interaction

2018 ◽  
Vol 42 (20) ◽  
pp. 17115-17125 ◽  
Author(s):  
K. S. Bezerra ◽  
J. X. Lima Neto ◽  
J. I. N. Oliveira ◽  
E. L. Albuquerque ◽  
E. W. S. Caetano ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Triple Helix ◽  
Complex Interaction ◽  
Computational Investigation ◽  
Protein Protein Interactions ◽  
Important Protein ◽  
Collagen Triple Helix ◽  
Α2β1 Integrin

In this paper, quantum biochemistry methods have been used to describe important protein–protein interactions for the complex integrin–collagen.

Protein–protein interactions in polyketide synthase–nonribosomal peptide synthetase hybrid assembly lines

2018 ◽  
Vol 35 (11) ◽  
pp. 1185-1209 ◽  
Author(s):  
Akimasa Miyanaga ◽  
Fumitaka Kudo ◽  
Tadashi Eguchi
Keyword(s):  
Protein Interactions ◽  
Polyketide Synthase ◽  
Nonribosomal Peptide Synthetase ◽  
Hybrid Assembly ◽  
Protein Protein Interactions ◽  
Assembly Lines ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase

The protein–protein interactions in polyketide synthase–nonribosomal peptide synthetase hybrids are summarized and discussed.

scholarly journals Explaining RANKL inhibition by OPG through quantum biochemistry computations and insights into peptide-design for the treatment of osteoporosis

RSC Advances ◽  
2016 ◽  
Vol 6 (88) ◽  
pp. 84926-84942 ◽  
Author(s):  
Bruno L. Sousa ◽  
Ito L. Barroso-Neto ◽  
Evanildo F. Oliveira ◽  
Emerson Fonseca ◽  
Pedro Lima-Neto ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Peptide Design ◽  
Protein Protein Interactions ◽  
Treatment Of Osteoporosis ◽  
Inhibitory Peptides ◽  
Important Protein

Quantum biochemistry computations are applied to precisely describe important protein–protein interactions, providing a basis for the design of inhibitory peptides against osteoporosis.

scholarly journals Mapping the biosynthetic pathway of a hybrid polyketide-nonribosomal peptide in a metazoan

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Likui Feng ◽  
Matthew T. Gordon ◽  
Ying Liu ◽  
Kari B. Basso ◽  
Rebecca A. Butcher
Keyword(s):  
Protein Interactions ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Carrier Protein ◽  
Nonribosomal Peptides ◽  
Direct Transfer ◽  
Protein Protein Interactions ◽  
Nonribosomal Peptide ◽  
Peptide Synthetase ◽  
Complex Protein

AbstractPolyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems typically use complex protein-protein interactions to facilitate direct transfer of intermediates between these multimodular megaenzymes. In the canal-associated neurons (CANs) of Caenorhabditis elegans, PKS-1 and NRPS-1 produce the nemamides, the only known hybrid polyketide-nonribosomal peptides biosynthesized by animals, through a poorly understood mechanism. Here, we use genome editing and mass spectrometry to map the roles of individual PKS-1 and NRPS-1 enzymatic domains in nemamide biosynthesis. Furthermore, we show that nemamide biosynthesis requires at least five additional enzymes expressed in the CANs that are encoded by genes distributed across the worm genome. We identify the roles of these enzymes and discover a mechanism for trafficking intermediates between a PKS and an NRPS. Specifically, the enzyme PKAL-1 activates an advanced polyketide intermediate as an adenylate and directly loads it onto a carrier protein in NRPS-1. This trafficking mechanism provides a means by which a PKS-NRPS system can expand its biosynthetic potential and is likely important for the regulation of nemamide biosynthesis.

scholarly journals Protein–protein interactions in “cis-AT” polyketide synthases

2018 ◽  
Vol 35 (10) ◽  
pp. 1082-1096 ◽  
Author(s):  
Greg J. Dodge ◽  
Finn P. Maloney ◽  
Janet L. Smith
Keyword(s):  
Protein Interactions ◽  
Active Site ◽  
Catalytic Domain ◽  
Polyketide Synthases ◽  
Protein Protein Interactions ◽  
P Protein ◽  
In Cis

Protein–protein interactions of cis-AT polyketide synthases are dominated by the travels of the ACP domain to the active site entrance of each catalytic domain.

Sign in / Sign up

Export Citation Format

Share Document