Phosphorylated chitosan to promote biomimetic mineralization of type I collagen as a strategy for dentin repair and bone tissue engineering

2019 ◽  
Vol 43 (4) ◽  
pp. 2002-2010 ◽  
Author(s):  
Bo Zheng ◽  
Caiyun Mao ◽  
Tianyi Gu ◽  
Haihua Pan ◽  
Changyu Shao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Efficient Method ◽  
Type I Collagen ◽  
Type I ◽  
Biomimetic Mineralization ◽  
Mineralized Matrix

This novel biomimetic mineralization technique provides an efficient method to produce an advanced mineralized matrix.

scholarly journals Development and Biocompatibility of Collagen-Based Composites Enriched with Nanoparticles of Strontium Containing Mesoporous Glass

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3719 ◽  
Author(s):  
Giorgia Montalbano ◽  
Giorgia Borciani ◽  
Carlotta Pontremoli ◽  
Gabriela Ciapetti ◽  
Monica Mattioli-Belmonte ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Bone Tissue Regeneration ◽  
In Vitro Tests ◽  
Type I ◽  
Natural Polymers ◽  
Bioactive Glasses ◽  
Composition And Structure

In the last years bone tissue engineering has been increasingly indicated as a valid solution to meet the challenging requirements for a healthy bone regeneration in case of bone loss or fracture. In such a context, bioactive glasses have already proved their great potential in promoting the regeneration of new bone tissue due to their high bioactivity. In addition, their composition and structure enable us to incorporate and subsequently release therapeutic ions such as strontium, enhancing the osteogenic properties of the material. The incorporation of these inorganic systems in polymeric matrices enables the formulation of composite systems suitable for the design of bone scaffolds or delivery platforms. Among the natural polymers, type I collagen represents the main organic phase of bone and thus is a good candidate to develop biomimetic bioactive systems for bone tissue regeneration. However, alongside the specific composition and structure, the key factor in the design of new biosystems is creating a suitable interaction with cells and the host tissue. In this scenario, the presented study aimed at combining nano-sized mesoporous bioactive glasses produced by means of a sol–gel route with type I collagen in order to develop a bioactive hybrid formulation suitable for bone tissue engineering applications. The designed system has been fully characterized in terms of physico-chemical and morphological analyses and the ability to release Sr2+ ions has been studied observing a more sustained profile in presence of the collagenous matrix. With the aim to improve the mechanical and thermal stability of the resulting hybrid system, a chemical crosslinking approach using 4-star poly (ethylene glycol) ether tetrasuccinimidyl glutarate (4-StarPEG) has been explored. The biocompatibility of both non-crosslinked and 4-StarPEG crosslinked systems was evaluated by in vitro tests with human osteoblast-like MG-63 cells. Collected results confirmed the high biocompatibility of composites, showing a good viability and adhesion of cells when cultured onto the biomaterial samples.

Collagen-Inspired Nano-fibrous Poly(L-lactic acid) Scaffolds for Bone Tissue Engineering Created from Reverse Solid Freeform Fabrication

2004 ◽  
Vol 823 ◽  
Author(s):  
Victor J. Chen ◽  
Laura A. Smith ◽  
Peter X. Ma
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Computer Aided Design ◽  
Type I Collagen ◽  
Polymer Concentration ◽  
Three Dimensional ◽  
Type I ◽  
Fibrous Scaffold

AbstractReverse solid freeform (SFF) fabrication was used to create highly-controlled macroporous structures in nano-fibrous poly (L-lactic acid) (PLLA) scaffolds. By using a computer-aided design (CAD) program to create a negative template for the scaffold, the three-dimensional (3-D) mold was created on a 3-D printer using a wax. After the template was printed, a solution of PLLA in tetrahydrofuran (THF) was cast into the mold, and was subsequently phase separated at -70°C which gives the nano-fibrous morphology. This resulted in a 3-D nano-fibrous scaffold with a uniform fiber mesh throughout the entire matrix, and greatly increased the surface area within the scaffold. Fiber diameters in these scaffolds were 50-500 nm, similar to type I collagen, and the densities of the fiber meshes can be altered by changing the polymer concentration. To examine the scaffold's potential for tissue regeneration, MC3T3-E1 osteoblasts were seeded and cultured on the scaffolds. Results show that the osteoblasts attached and proliferated on the scaffolds. After 6 weeks in culture, bone-like tissue was evident within the nano-fibrous scaffolds. By having the ability to control the macroporous architecture, interconnectivity, orientation, and external shape of the scaffold, as well as the nanometer-scaled fibrous features in the pore walls, this SFF fabrication/phase separation technique has great potential to design and create ideal scaffolds for bone tissue engineering.

Investigation on the Biomimetic Scaffold for Bone Tissue Engineering Based on Bioglass-Collagen-Hyaluronic Acid-Phosphatidylserine

2007 ◽  
Vol 330-332 ◽  
pp. 939-942 ◽  
Author(s):  
Xiao Feng Chen ◽  
Ying Jun Wang ◽  
Na Ru Zhao ◽  
Chun Rong Yang
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Porous Structure ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Type I ◽  
High Porosity ◽  
Culture Methods ◽  
Biomimetic Scaffold

The new type of bone tissue engineering scaffold composed of the sol-gel derived bioactive glass particles, type I collagen, hyaluronic acid and phosphatidylserine were prepared through cross-linking and freeze-drying techniques. SEM observation indicated that the scaffold possessed a 3-D interconnected porous structure and a high porosity. The properties of bio-mineralization and cells biocompatibility were investigated using SBF immersion and cells culture methods combined with SEM, XRD and FTIR techniques. The study revealed that this biomimetic scaffold possessed satisfactory functions of cells attachment, bio-mineralization, and cells biocompatibility. The porous structure and the surface of the scaffold which was covered by a bone-like HA crystal layer due to bio-mineralization were profitable for cells attachment and spread.

Biomimetic Fabrication and Characterization of BG/COL/HCA Scaffolds for Bone Tissue Engineering

2007 ◽  
Vol 336-338 ◽  
pp. 1574-1576
Author(s):  
Xiao Feng Chen ◽  
Ying Jun Wang ◽  
Chun Rong Yang ◽  
Na Ru Zhao
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Porous Scaffold ◽  
Sol Gel ◽  
Type I ◽  
Carbonate Apatite

The bone tissue engineering scaffold was developed by compounded the type I collagen with the porous scaffold of the sol-gel derived bioactive glass (BG) in the system CaO-P2O5-SiO2. The resultant porous scaffold was treated in supersaturated calcification solution (SCS) to form the surface layer of hydroxyl-carbonate-apatite (HCA) since the type I collagen possessed good biocompatibility and bio-absorbability, and also, the ability of inducting calcium phosphates to precipitated inside and outside the collagen fibers where the collagen fibers acted as bio-macromolecules template for formation of bone-like inorganic minerals in nature bone such as: octo-calcium phosphate (OCP), tri-calcium phosphate (TCP) and hydroxyl-carbonate-apatite (HCA). On the other hand, the sol-gel derived bioactive glass also played an important role in formation of the above bio-minerals owing to its serial chemical reactions with the body fluid. The in vitro study in supersaturated calcification solution SCS indicated that the surface of the porous scaffold was able to induce formation of bone-like HCA crystals on the pore walls of the scaffold which possessed satisfactory cells biocompatibility.

The Application of Biological Materials on the Cruciate Ligament Reconstruction of Knee-Joint in Athletic Injury

2013 ◽  
Vol 643 ◽  
pp. 25-28
Author(s):  
Kai Liu
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Surface Topology ◽  
Future Research ◽  
Material Surface ◽  
Type I ◽  
Modification Of Materials

It is Important content that to make Surface modification and surface modification and Improve the material on the surface of the cell adhesion and promotes cell proliferation to bone tissue engineering scaffolds. The role of osteoblast and support material dependent on the Material surface characteristics, Local shape, surface energy and chemical energy, which Determine how cells adsorbed onto the surface of the material and Localization of cells and cell function behavior. Therefore, the complexity of biomaterials and cell biological material surface interaction determines the biological scaffold materials for surface modification of importance. Ideal surface modification should take into consideration the surface topology, specific identification, hydrophilic and hydrophobic protein adsorption equilibrium, and other aspects of functional new tissues. At present, the most applications in surface modification of materials is type I collagen, future research will be a variety of surface modification of materials for composite materials, which will play complementary roles, as well as gene therapy and the development of nanometer materials, it will become a hot issue in the field of bone tissue engineering.

Biomimetic Mineralization of Hydrogel Biomaterials for Bone Tissue Engineering

Biomimetics ◽  
2013 ◽  
pp. 51-67
Author(s):  
Timothy E.L. Douglas ◽  
Elzbieta Pamula ◽  
Sander C.G. Leeuwenburgh
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomimetic Mineralization

Fabrication of a RP-Based Biomimetic Grafting Material for Bone Tissue Engineering

2009 ◽  
Vol 626-627 ◽  
pp. 553-558 ◽  
Author(s):  
Xing Ma ◽  
Y.Y. Hu ◽  
Xiao Ming Wu ◽  
J. Liu ◽  
Zhuo Xiong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Type ◽  
Autologous Bone Marrow ◽  
Collagen Type I ◽  
Control Group ◽  
Type I ◽  
High Porosity ◽  
Highly Porous

Three-dimensional (3D) highly porous poly (DL-lactic-co-glycolic acid)/tricalcium phosphate (PLGA/TCP) scaffolds were fabricated using a rapid prototyping technique (RP). The biopolymer carriers (4mm×4mm×4mm) subsequently were coated with collagen type I (Col) to produce PLGA/TCP/Col composites and utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Autologous bone marrow stromal cells (BMSCs) harvested from New Zealand white rabbits were cultured under an osteogenic condition (BMSCs-OB) followed by seeding into the structural highly porous PLGA/TCP/Col composites (i.e. PLGA/TCP/Col/BMSCs-OB). Scanning electron microscopy observation found that the RP-based scaffolds had appropriate microstructure, controlled interconnectivity and high porosity. Modification of the scaffolds with collagen type I (PLGA/TCP/Col) essentially increased the affinity of the carriers to seeding cells, and PLGA/TCP/Col composites were well biocompatible with BMSCs-OB. The PLGA/TCP/Col/BMSCs-OB constructs were then subcutaneously implanted in the back of rabbits compared to controls with autologous BMSCs suspension and carriers alone. As a result, histological new bone formation was observed only in the experimental group with PLGA/TCP/Col/BMSCs-OB constructs 8 weeks after implantation. In the control group with scaffold alone only biodegradation of the carriers was found. Therefore, these results validate our bio-manufacturing methods for a new bone graft substitute.

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