scholarly journals Modular assembly of plasmonic core–satellite structures as highly brilliant SERS-encoded nanoparticles

2019 ◽  
Vol 1 (1) ◽  
pp. 122-131 ◽  
Author(s):  
Nicolas Pazos-Perez ◽  
Jamie M. Fitzgerald ◽  
Vincenzo Giannini ◽  
Luca Guerrini ◽  
Ramon A. Alvarez-Puebla
Keyword(s):  
Particle Loading ◽  
Modular Assembly ◽  
Homogeneous Core ◽  
Maximum Particle ◽  
Encoded Particles

Herein, we present a fabrication approach that produces homogeneous core–satellite SERS encoded particles with minimal interparticle gaps (<2–3 nm) and maximum particle loading, while positioning the encoding agents at the gaps.

Quantities Directly Measurable by Scattering

2018 ◽  
Author(s):  
Eaton E. Lattman ◽  
Thomas D. Grant ◽  
Edward H. Snell
Keyword(s):  
Molecular Weight ◽  
Particle Shape ◽  
Particle Surface ◽  
Scattering Data ◽  
Radius Of Gyration ◽  
Particle Volume ◽  
Particle Surface Area ◽  
Scattering Angle ◽  
Particle Dimension ◽  
Maximum Particle

In this chapter we note that solution scattering data can be divided into four regions. At zero scattering angle, the scattering provides information on molecular weight of the particle in solution. Beyond that, the scattering is influenced by the radius of gyration. As the scattering angle increases, the scattering is influenced by the particle shape, and finally by the interface with the particle and the solution. There are a number of important invariants that can be calculated directly from the data including molecular mass, radius of gyration, Porod invariant, particle volume, maximum particle dimension, particle surface area, correlation length, and volume of correlation. The meaning of these is described in turn along with their mathematical derivations.

scholarly journals Influence of protein (human galectin-3) design on aspects of lectin activity

2020 ◽  
Vol 154 (2) ◽  
pp. 135-153 ◽  
Author(s):  
Gabriel García Caballero ◽  
Donella Beckwith ◽  
Nadezhda V. Shilova ◽  
Adele Gabba ◽  
Tanja J. Kutzner ◽  
...  
Keyword(s):  
Protein Design ◽  
Full Range ◽  
Biological Information ◽  
The Novel ◽  
Modular Assembly ◽  
Binding Partners ◽  
Galectin 3 ◽  
Potential Applications ◽  
Similar Affinity

Abstract The concept of biomedical significance of the functional pairing between tissue lectins and their glycoconjugate counterreceptors has reached the mainstream of research on the flow of biological information. A major challenge now is to identify the principles of structure–activity relationships that underlie specificity of recognition and the ensuing post-binding processes. Toward this end, we focus on a distinct feature on the side of the lectin, i.e. its architecture to present the carbohydrate recognition domain (CRD). Working with a multifunctional human lectin, i.e. galectin-3, as model, its CRD is used in protein engineering to build variants with different modular assembly. Hereby, it becomes possible to compare activity features of the natural design, i.e. CRD attached to an N-terminal tail, with those of homo- and heterodimers and the tail-free protein. Thermodynamics of binding disaccharides proved full activity of all proteins at very similar affinity. The following glycan array testing revealed maintained preferential contact formation with N-acetyllactosamine oligomers and histo-blood group ABH epitopes irrespective of variant design. The study of carbohydrate-inhibitable binding of the test panel disclosed up to qualitative cell-type-dependent differences in sections of fixed murine epididymis and especially jejunum. By probing topological aspects of binding, the susceptibility to inhibition by a tetravalent glycocluster was markedly different for the wild-type vs the homodimeric variant proteins. The results teach the salient lesson that protein design matters: the type of CRD presentation can have a profound bearing on whether basically suited oligosaccharides, which for example tested positively in an array, will become binding partners in situ. When lectin-glycoconjugate aggregates (lattices) are formed, their structural organization will depend on this parameter. Further testing (ga)lectin variants will thus be instrumental (i) to define the full range of impact of altering protein assembly and (ii) to explain why certain types of design have been favored during the course of evolution, besides opening biomedical perspectives for potential applications of the novel galectin forms.

Modular assembly of enzyme loaded nanoparticles in 3D hollow fiber electrode for electrochemical sensing

2021 ◽  
pp. 129721
Author(s):  
Huimin Wu ◽  
Xinran Zhang ◽  
Chenjie Wei ◽  
Chengcheng Wang ◽  
Min Jiang ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Electrochemical Sensing ◽  
Modular Assembly ◽  
Fiber Electrode

Insight into the tannic acid-based modular-assembly strategy based on inorganic–biological hybrid systems: a material suitability, loading effect, and biocompatibility study

2021 ◽  
Author(s):  
Xiaoning Ren ◽  
Panqing Yin ◽  
Jun Liang ◽  
Xiangjian Liu ◽  
Wugen Zhan ◽  
...  
Keyword(s):  
Hybrid Systems ◽  
Tannic Acid ◽  
Modular Assembly ◽  
Loading Effect ◽  
Assembly Strategy ◽  
Biocompatibility Study ◽  
Insight Into

The tannic acid-based modular-assembly strategy for building inorganic–biological hybrids is studied regarding the aspects of the material suitability, loading effect, and biocompatibility.

Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum

2021 ◽  
Vol 53 ◽  
pp. 102159
Author(s):  
Matthias Windhagauer ◽  
Raffaela M. Abbriano ◽  
Justin Ashworth ◽  
Lorenzo Barolo ◽  
Ana Cristina Jaramillo-Madrid ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Regulatory Sequences ◽  
Modular Assembly

Effects of maximum particle size of coarse aggregates and steel fiber contents on the mechanical properties and impact resistance of recycled aggregate concrete

2021 ◽  
pp. 136943322110179
Author(s):  
DongTao Xia ◽  
ShaoJun Xie ◽  
Min Fu ◽  
Feng Zhu
Keyword(s):  
Mechanical Properties ◽  
Particle Size ◽  
Steel Fiber ◽  
Impact Resistance ◽  
Recycled Aggregate Concrete ◽  
Recycled Aggregate ◽  
Aggregate Concrete ◽  
Coarse Aggregates ◽  
Maximum Particle Size ◽  
Maximum Particle

Fiber reinforced recycled aggregate concrete has become a new type of green concrete material. The maximum particle size of coarse aggregates and steel fiber contents affect the mechanical properties and impact resistance of recycled aggregate concrete. However, such studies are rare in literature. The present paper shortens the gap through experimental study. A total of 144 specimens of 12 kinds of concrete mixtures were tested, which adopted different steel fiber volume admixtures (0%, 0.8%, 1.0%, 1.2%) and recycled coarse aggregates in different maximum particle sizes (9.5, 19, 31.5 mm) replacing 30% natural coarse aggregate. The compressive strength, splitting tensile strength, and impact resistance of the 12 concrete mixtures were tested. The results showed that the compressive strength, splitting tensile strength, and impact resistance of recycled aggregate concrete increased first and then decreased with the increase of the maximum particle size. The recycled aggregate concrete with the maximum particle size of 19 mm had the highest mechanical properties and impact resistance. Besides, with the increase of steel fiber content, the compressive strength, splitting tensile strength, and impact resistance of recycled aggregate concrete showed an increasing trend. Considering a large amount of experimental data and the coupling effect of steel fiber contents and the maximum particle size of coarse aggregates, the Weibull distribution function was introduced to analyze the impact test results and predict the number of resistance to impact under different failure probabilities. The results showed that the number of blows of the recycled aggregate concrete followed a two-parameter Weibull distribution, and the estimated value of the number of resistance to impact for failure increased with the increase of the failure probability.

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