scholarly journals In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells

2019 ◽  
Vol 1 (1) ◽  
pp. 367-377 ◽  
Author(s):  
Sumaira Ashraf ◽  
Arthur Taylor ◽  
Jack Sharkey ◽  
Michael Barrow ◽  
Patricia Murray ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Intracardiac Injection

Free and encapsulated iron oxide nanoparticles after intracardiac injection of labelled stem cells have similar in vivo fate.

Magnetic Resonance Imaging Tracking of Stem Cells in Vivo Using Iron Oxide Nanoparticles as a Tool for the Advancement of Clinical Regenerative Medicine

2011 ◽  
Vol 111 (2) ◽  
pp. 253-280 ◽  
Author(s):  
Morteza Mahmoudi ◽  
Hossein Hosseinkhani ◽  
Mohsen Hosseinkhani ◽  
Sebastien Boutry ◽  
Abdolreza Simchi ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Stem Cells ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Regenerative Medicine ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Resonance Imaging

scholarly journals In vivo fate of free and encapsulated iron oxide nanoparticles after injection of labelled stem cells

2018 ◽  
Author(s):  
Sumaira Ashraf ◽  
Arthur Taylor ◽  
Jack Sharkey ◽  
Michael Barrow ◽  
Patricia Murray ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Inductively Coupled Plasma ◽  
Superparamagnetic Iron Oxide ◽  
Emission Spectrometry ◽  
Magnetic Characteristics ◽  
Oxide Nanoparticles ◽  
Imaging Results

AbstractNanoparticle contrast agents are useful tools to label stem cells and monitor the in vivo bio-distribution of labeled cells in pre-clinical models of disease. In this context, understanding the in vivo fate of the particles after injection of labelled cells is important for their eventual clinical use as well as for the interpretation of imaging results. We examined how the formulation of superparamagnetic iron oxide nanoparticles (SPIONs) impacts the labelling efficiency, magnetic characteristics and fate of the particles by comparing individual SPIONs with polyelectrolyte multilayer capsules containing SPIONs. At low labelling concentration, encapsulated SPIONs served as an efficient labelling agent for stem cells. The bio-distribution after intra-cardiac injection of labelled cells was monitored longitudinally by MRI and as an endpoint by inductively coupled plasma-optical emission spectrometry. The results suggest that, after being released from labelled cells after cell death, both formulations of particles are initially stored in liver and spleen and are not completely cleared from these organs 2 weeks post-injection.

Superparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo

2018 ◽  
Vol 46 (1) ◽  
pp. 191-198 ◽  
Author(s):  
Ricardo Luiz Azevedo-Pereira ◽  
Bárbara Rangel ◽  
Fernanda Tovar-Moll ◽  
Emerson Leandro Gasparetto ◽  
Marcia Attias ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Neural Stem Cells ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals In vivo Uptake of BrdU, GFP and Iron Oxide Nanoparticles by Local Tissue Macrophages from Labeled Stem Cells. Implications for Cell Transplantation

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Edyta Pawelczyk ◽  
Eleine Kay Jordan ◽  
Janna Johnson‐Barelo ◽  
Melissa Smith ◽  
Aneeka Chaudhry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Iron Oxide ◽  
Cell Transplantation ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Local Tissue ◽  
In Vivo Uptake

scholarly journals Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 484
Author(s):  
Yue Gao ◽  
Anna Jablonska ◽  
Chengyan Chu ◽  
Piotr Walczak ◽  
Miroslaw Janowski
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Extracellular Vesicles ◽  
Superparamagnetic Iron Oxide ◽  
Cell Labeling ◽  
Oxide Nanoparticles ◽  
Stem Cell Delivery ◽  
Cellular Labeling

Rapidly ageing populations are beset by tissue wear and damage. Stem cell-based regenerative medicine is considered a solution. Years of research point to two important aspects: (1) the use of cellular imaging to achieve sufficient precision of therapeutic intervention, and the fact that (2) many therapeutic actions are executed through extracellular vesicles (EV), released by stem cells. Therefore, there is an urgent need to interrogate cellular labels in the context of EV release. We studied clinically applicable cellular labels: superparamagnetic iron oxide nanoparticles (SPION), and radionuclide detectable by two main imaging modalities: MRI and PET. We have demonstrated effective stem cell labeling using both labels. Then, we obtained EVs from cell cultures and tested for the presence of cellular labels. We did not find either magnetic or radioactive labels in EVs. Therefore, we report that stem cells do not lose labels in released EVs, which indicates the reliability of stem cell magnetic and radioactive labeling, and that there is no interference of labels with EV content. In conclusion, we observed that direct cellular labeling seems to be an attractive approach to monitoring stem cell delivery, and that, importantly, labels neither locate in EVs nor affect their basic properties.

Sign in / Sign up

Export Citation Format

Share Document