scholarly journals Sub-picogram level sensitivity in HIV diagnostics achieved with the europium nanoparticle immunoassay through metal enhanced fluorescence

2019 ◽  
Vol 1 (1) ◽  
pp. 273-280 ◽  
Author(s):  
Aditya Kurdekar ◽  
L. A. Avinash Chunduri ◽  
Mohan Kumar Haleyurgirisetty ◽  
Indira K. Hewlett ◽  
Venkataramaniah Kamisetti
Keyword(s):  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Hiv Diagnostics

Gold nanoparticle based metal enhanced fluorescence applied to the Europium Nanoparticle Immunoassay (ENIA) improves the limit of detection by ten-fold from 1.8 pg mL−1to 0.19 pg mL−1.

scholarly journals Plasmonic Hydrogel Nanocomposites with Combined Optical and Mechanical Properties for Biochemical Sensing

2021 ◽  
Vol 5 (1) ◽  
pp. 34
Author(s):  
Bruno Miranda ◽  
Rosalba Moretta ◽  
Selene De Martino ◽  
Principia Dardano ◽  
Ilaria Rea ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Limit Of Detection ◽  
Noble Metal Nanoparticles ◽  
Localized Surface Plasmon ◽  
Diagnostic Tools ◽  
Label Free ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

Localized surface plasmon resonance (LSPR) and metal-enhanced-fluorescence (MEF)-based optical biosensors exhibit unique properties compared to other sensing devices that can be exploited for the design point-of-care (POC) diagnostic tools [1]. Plasmonic devices exploit the capability of noble-metal nanoparticles of absorbing light at a well-defined wavelength. The increasing request for wearable, flexible and easy-to-use diagnostic tools has brought to the development of plasmonic nanocomposites, whose peculiar performances arise from the combination of the optical properties of plasmonic nanoparticles and mechanical properties of the polymeric matrix in which they are embedded [2,3]. An optical platform based on spherical gold nanoparticles (AuNPs) embedded in high molecular weight poly-(ethylene glycol) diacrylate (PEGDA) hydrogel is proposed. PEGDA hydrogel represents a biocompatible, flexible, transparent polymeric network to design wearable, 3D, plasmonic biosensors for the detection of targets with different molecular weights for the early diagnosis of disease. The swelling capability of PEGDA is directly correlated to the plasmonic decoupling of AuNPs embedded within the matrix. A study on the effect of swelling on the optical response of the PEGDA/AuNPs composites was investigated by using a biorecognition layer/target model system. Specifically, after the in situ chemical modification of the AuNPs within the hydrogel, the interaction biotin-streptavidin is monitored within the 3D hydrogel network. Additionally, metal-enhanced fluorescence is observed within the PEGDA/AuNPs nanocomposites, which can be exploited to achieve an ultra-low limit of detection. LSPR signal was monitored via transmission mode customized setup and MEF signal was detected via fluorescence and confocal microscopes. Label-free (LSPR-based) and fluorescence (MEF-based) signals of a high molecular weight target analyte were successfully monitored with relatively high resolutions and low limits of detection compared to the standard polymeric optical platforms available in the literature. The optimized platform could represent a highly reproducible and low-cost novel biosensor to be applied as a POC diagnostic tool in healthcare and food monitoring applications.

Metal-enhanced fluorescence from tin nanostructured surfaces

2010 ◽  
Vol 107 (2) ◽  
pp. 024302 ◽  
Author(s):  
Yongxia Zhang ◽  
Anatoliy Dragan ◽  
Chris D. Geddes
Keyword(s):  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Nanostructured Surfaces

UV-Trained and Metal-Enhanced Fluorescence of Biliverdin and Biliverdin Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Parinaz Fathi ◽  
Ayman Roslend ◽  
Kritika Mehta ◽  
Parikshit Moitra ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Biomedical Imaging ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

Increasing the fluorescence quantum yield of fluorophores is of great interest for in vitro and in vivo biomedical imaging applications. At the same time, photobleaching and photodegradation resulting from continuous...

A benzothiazole-based fluorescence turn-on sensor for copper(II)

2021 ◽  
Author(s):  
Gyeongjin Kim ◽  
Donghwan Choi ◽  
Cheal Kim
Keyword(s):  
Charge Transfer ◽  
Limit Of Detection ◽  
Theoretical Calculations ◽  
Enhanced Fluorescence ◽  
Turn On ◽  
Fluorescent Response ◽  
Chelation Enhanced Fluorescence ◽  
Fluorescence Turn On ◽  
Internal Charge Transfer ◽  
Internal Charge

Abstract A new benzothiazole-based chemosensor BTN (1-((Z)-(((E)-3-methylbenzo[d]thiazol-2(3H)-ylidene)hydrazono)methyl)naphthalen-2-ol) was synthesized for the detection of Cu2+. BTN could detect Cu2+ with “off-on” fluorescent response from colorless to yellow irrespective of presence of other cations. Limit of detection for Cu2+ was determined to be 3.3 µM. Binding ratio of BTN and Cu2+ turned out to be a 1:1 with the analysis of Job plot and ESI-MS. Sensing feature of Cu2+ by BTN was explained with theoretical calculations, which might be owing to internal charge transfer and chelation-enhanced fluorescence processes.

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