Body distribution of stable copper isotopes during the progression of cholestatic liver disease induced by common bile duct ligation in mice

Metallomics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1093-1103 ◽  
Author(s):  
Marta Costas-Rodríguez ◽  
Sanne Van Campenhout ◽  
Agustina A. M. B. Hastuti ◽  
Lindsey Devisscher ◽  
Hans Van Vlierberghe ◽  
...  
Keyword(s):  
Liver Disease ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Duct Ligation ◽  
The Body ◽  
Cholestatic Liver Disease ◽  
Common Bile Duct Ligation ◽  
Body Distribution ◽  
Duct Ligation ◽  
Severity Of The Disease

The effect of cholestatic liver disease on the body Cu isotopic distribution was investigated in a common bile duct ligation mouse model. The isotopic composition of Cu in serum and organs becomes gradually lighter with increasing severity of the disease.

Plasma Membrane Form of Phosphatidate Phosphohydrolase: A Possible Role in Signal Transduction during Liver Fibrogenesis

1993 ◽  
Vol 85 (3) ◽  
pp. 281-287 ◽  
Author(s):  
Christopher P. Day ◽  
Alastair D. Burt ◽  
Ashley Stj. M. Brown ◽  
Mark K. Bennett ◽  
Desmond J. Farrell ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Liver Disease ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Alcoholic Liver Disease ◽  
Bile Duct Ligation ◽  
Sham Operation ◽  
Common Bile Duct Ligation ◽  
Duct Ligation ◽  
Phosphatidate Phosphohydrolase

1. Several growth factors important in liver regeneration and fibrosis stimulate phospholipase D in plasma membranes via a receptor/G-protein-coupled mechanism resulting in hydrolysis of phosphatidylcholine to phosphatidate. Phosphatidate can be further hydrolysed to diacylglycerol by phosphatidate phosphohydrolase. Phosphatidate and diacylglycerol can act as ‘second-messengers’ and regulation of phosphatidate phosphohydrolase activity could control the balance between them. 2. A form of phosphatidate phosphohydrolase, located in the plasma membrane and insensitive to inhibition by N-ethylmaleimide, has recently been identified that is distinct from the ‘metabolic’ form, which is present in the cytosol and microsomes and is sensitive to N-ethylmaleimide. 3. We have investigated the hypothesis that the balance between regeneration and fibrosis is, in part, determined by the activity of plasma membrane phosphatidate phosphohydrolase through its effect on the phosphatidate/diacylglycerol ratio. N-Ethylmaleimide-insensitive and -sensitive phosphatidate phosphohydrolase activities were measured in three hepatic conditions characterized by regeneration and/or fibrosis: alcoholic liver disease in humans (regeneration and fibrosis) and rat livers after either acute CCl-4-induced injury (regeneration) or common bile duct ligation (fibrosis). 4. In patients with alcoholic liver disease, N-ethylmaleimide-insensitive phosphatidate phosphohydrolase activity was higher in cirrhotic biopsies (5.82±0.3 nmol of Pi min−1 mg−1 of protein, n = 19) than in non-cirrhotic biopsies (2.17 ±0.2, n = 23) or in wedge biopsies from healthy subjects undergoing routine cholecystectomy (2.16 ±0.5, n = 6). N-Ethylmaleimide-insensitive phosphatidate phosphohydrolase activity was unchanged in the 10 days after CCl4 treatment but increased progressively in common bile duct-ligated rats (e.g. day 28: ‘sham’ operation, 1.97 ±0.3, chronic bile duct ligation, 6.91 ±1.24 nmol of Pi min−1 mg−1 of protein). N-Ethylmaleimide-insensitive phosphatidate phosphohydrolase activity correlated closely with the degree of fibrosis in humans and rats. N-Ethylmaleimide-sensitive phosphatidate phosphohydrolase activity was unchanged after CCI4 treatment or common bile duct ligation and was not increased in cirrhotic livers. 5. Plasma membrane N-ethylmaleimide-insensitive phosphatidate phosphohydrolase increases in liver fibrosis but not regeneration. Stimulation of phosphatidate phosphohydrolase activity with its effect on the diacylglycerol/phosphatidate ratio may play a role in transduction of the fibrosis signal.

scholarly journals Pathophysiology of Lung Injury Induced by Common Bile Duct Ligation in Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94550 ◽  
Author(s):  
Fumiaki Shikata ◽  
Tomohisa Sakaue ◽  
Koh-ichi Nakashiro ◽  
Mikio Okazaki ◽  
Mie Kurata ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Lung Injury ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Duct Ligation

scholarly journals A new model of reversible obstructive jaundice using rapidly absorbable suture materials

2012 ◽  
Vol 35 (6) ◽  
pp. 351 ◽  
Author(s):  
Nurettin Kahramansoy ◽  
Hayri Erkol ◽  
Edip E Yilmaz ◽  
Mustafa Şit ◽  
Fahri Yilmaz ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Obstructive Jaundice ◽  
Surgical Intervention ◽  
Bile Duct Ligation ◽  
Absorbable Suture ◽  
Suture Materials ◽  
Common Bile Duct Ligation ◽  
Duct Ligation ◽  
Morphologic Changes

Purpose: Reversible obstructive jaundice models have some limiting features, including the need for a second anaesthesia, re-laparotomy and surgical intervention after common bile duct ligation. The present study investigates the feasibility of a new application that can eliminate these limitations. Rapidly absorbable suture materials were used for ligation; therefore, spontaneous biliary decompression was anticipated by the self release of these rapidly degrading materials. Methods: Common bile ducts in Wistar Albino rats were ligated with silk, polyglytone 6211, or irradiated polyglactine 910 (n=7 for each group). Rats were grouped according to both the suture materials and the experiments termination date: 5 days (sham, silk5, polyglytone5, polyglactine5) and 21 days (silk21, polyglytone21, polyglactine21) after the ligation. Biochemical and morphologic changes of liver were assessed. Results: The group polyglactine21 showed significantly lower mean ALT, AST, GGT, total and direct bilirubin values when compared with the group polyglactine5 (p=0.004-0.037). Morphologic changes did not correlate with the biochemical amelioration. In the group polyglytone21, not only the biochemical but also the morphologic changes significantly ameliorated when compared with the group polyglytone5 (p=0.003-0.043). No procedure associated mortality was observed. Conclusion: Common bile duct ligation with polyglytone offers a new reversible model for prolonged obstructive jaundice which abolishes the need for relaparotomy and a second surgical intervention and significantly reduces mortality.

Characterization of inducible nature of MRP3 in rat liver

2000 ◽  
Vol 278 (3) ◽  
pp. G438-G446 ◽  
Author(s):  
Kotaro Ogawa ◽  
Hiroshi Suzuki ◽  
Tomoko Hirohashi ◽  
Toshihisa Ishikawa ◽  
Peter J. Meier ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Protein Level ◽  
Bile Duct Ligation ◽  
Organic Anion ◽  
Mrna Level ◽  
Common Bile Duct Ligation ◽  
Sd Rats ◽  
Anion Transporter ◽  
Duct Ligation

We found previously that expression of multidrug resistance-associated protein (MRP) 3 is induced in a mutant rat strain (Eisai hyperbilirubinemic rats) whose canalicular multispecific organic anion transporter (cMOAT/MRP2) function is hereditarily defective and in normal Sprague-Dawley (SD) rats after ligation of the common bile duct. In the present study, the inducible nature of MRP3 was examined, using Northern and Western blot analyses, in comparison with that of other secondary active [Na+-taurocholic acid cotransporting polypeptide (Ntcp), organic anion transporting polypeptide 1 (oatp1), and organic cation transporter (OCT1)] and primary active [P-glycoprotein (P-gp), cMOAT/MRP2, and MRP6] transporters. α-Naphthylisothiocyanate treatment and common bile duct ligation induced expression of P-gp and MRP3, whereas expression of Ntcp, oatp1, and OCT1 was reduced by the same treatment. Although expression of MRP3 was also induced by administration of phenobarbital, that of cMOAT/MRP2, MRP1, and MRP6 was not affected by any of these treatments. Moreover, the mRNA level of MRP3, but not that of P-gp, was increased in SD rats after administration of bilirubin and in Gunn rats whose hepatic bilirubin concentration is elevated because of a defect in the expression of UDP-glucuronosyl transferase. However, the MRP3 protein level was not affected by bilirubin administration. Although the increased MRP3 mRNA level was associated with the increased concentration of bilirubin and/or its glucuronides in mutant rats and in SD rats that had undergone common bile duct ligation or α-naphthylisothiocyanate treatment, we must assume that factor(s) other than these physiological substances are also involved in the increased protein level of MRP3.

Chondroitin sulfate protects liver in the experimental model of extra-hepatic cholestasis induced by common bile duct ligation

2017 ◽  
Vol 66 (1) ◽  
pp. S389
Author(s):  
P.L.R. Guedes ◽  
M.L. Gazarini ◽  
M.Y. Icimoto ◽  
J.A. Aguiar ◽  
M. Kouyoumdjian ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Chondroitin Sulfate ◽  
Experimental Model ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Duct Ligation
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