scholarly journals Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage

2019 ◽  
Vol 6 (2) ◽  
pp. 364-368 ◽  
Author(s):  
Daniel E. Mitchell ◽  
Alice E. R. Fayter ◽  
Robert C. Deller ◽  
Muhammad Hasan ◽  
Jose Gutierrez-Marcos ◽  
...  
Keyword(s):  
Antifreeze Protein ◽  
Solvent Free ◽  
Ice Recrystallization ◽  
Ice Growth ◽  
Freeze Stress

Antifreeze-protein mimic polymers are shown to enable solvent-free storage of important proteins for therapy and biotechnology by modulating ice growth.

Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl-based polymers

2014 ◽  
Vol 2 (12) ◽  
pp. 1787-1795 ◽  
Author(s):  
Daniel E. Mitchell ◽  
Mary Lilliman ◽  
Sebastian G. Spain ◽  
Matthew I. Gibson
Keyword(s):  
Growth Inhibition ◽  
Quantitative Study ◽  
Fish Species ◽  
Antifreeze Protein ◽  
Natural Sources ◽  
Ice Recrystallization ◽  
Ice Growth ◽  
Polar Fish

Antifreeze (glyco) proteins (AF(G)Ps) from the blood of polar fish species are extremely potent ice recrystallization inhibitors (IRI), but are difficult to synthesise or extract from natural sources.

scholarly journals Ice Growth Suppression in the Solution Flows of Antifreeze Protein and Sodium Chloride in a Mini-Channel

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 306
Author(s):  
Kazuya Taira ◽  
Tomonori Waku ◽  
Yoshimichi Hagiwara
Keyword(s):  
Sodium Chloride ◽  
Synergistic Effects ◽  
Pure Water ◽  
Interface Velocity ◽  
Antifreeze Protein ◽  
Layer Growth ◽  
Mixed Solution ◽  
Solution Flow ◽  
Ice Growth ◽  
Cold Energy

The control of ice growth inside channels of aqueous solution flows is important in numerous fields, including (a) cold-energy transportation plants and (b) the preservation of supercooled human organs for transplantation. A promising method for this control is to add a substance that influences ice growth in the flows. However, limited results have been reported on the effects of such additives. Using a microscope, we measured the growth of ice from one sidewall toward the opposite sidewall of a mini-channel, where aqueous solutions of sodium chloride and antifreeze protein flowed. Our aim was to considerably suppress ice growth by mixing the two solutes. Inclined interfaces, the overlapping of serrated interfaces, and interfaces with sharp and flat tips were observed in the cases of the protein-solution, salt-solution, and mixed-solution flows, respectively. In addition, it was found that the average interface velocity in the case of the mixed-solution flow was the lowest and decreased by 64% compared with that of pure water. This significant suppression of the ice-layer growth can be attributed to the synergistic effects of the ions and antifreeze protein on the diffusion of protein.

scholarly journals Modulating Intracellular Ice Growth with Cell-Permeating Small-Molecule Ice Recrystallization Inhibitors

Langmuir ◽  
2018 ◽  
Vol 35 (23) ◽  
pp. 7452-7458 ◽  
Author(s):  
Jessica S. Poisson ◽  
Jason P. Acker ◽  
Jennie G. Briard ◽  
Julia E. Meyer ◽  
Robert N. Ben
Keyword(s):  
Small Molecule ◽  
Ice Recrystallization ◽  
Ice Growth ◽  
Intracellular Ice

9. Cooperative ice growth inhibition of the isoforms of type III antifreeze protein

Cryobiology ◽  
2009 ◽  
Vol 59 (3) ◽  
pp. 372
Author(s):  
Manabu Takamichi ◽  
Yoshiyuki Nishimiya ◽  
Ai Miura ◽  
Sakae Tsuda
Keyword(s):  
Growth Inhibition ◽  
Antifreeze Protein ◽  
Type Iii ◽  
Ice Growth

scholarly journals Effects of Winter Flounder Antifreeze Protein on the Growth of Ice Particles in an Ice Slurry Flow in Mini-Channels

Biomolecules ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 70 ◽  
Author(s):  
Yuki Takeshita ◽  
Tomonori Waku ◽  
Peter W. Wilson ◽  
Yoshimichi Hagiwara
Keyword(s):  
Growth Inhibition ◽  
Antifreeze Protein ◽  
Winter Flounder ◽  
Ice Slurry ◽  
Protein Solution ◽  
Fresh Fish ◽  
Ice Growth ◽  
Ice Particles ◽  
Mini Channels ◽  
Ice Surfaces

The control of ice growth in ice slurry is important for many fields, including (a) the cooling of the brain during cardiac arrest, (b) the storage and transportation of fresh fish and fruits, and (c) the development of distributed air-conditioning systems. One of the promising methods for the control is to use a substance such as antifreeze protein. We have observed and report here growth states of ice particles in both quiescent and flowing aqueous solutions of winter flounder antifreeze proteins in mini-channels with a microscope. We also measured ice growth rates. Our aim was to improve the levels of ice growth inhibition by subjecting the antifreeze protein solution both to preheating and to concentrating by ultrafiltration. We have found that the ice growth inhibition by the antifreeze protein decreased in flowing solutions compared with that in quiescent solutions. In addition, unlike unidirectional freezing experiments, the preheating of the antifreeze protein solution reduced the ice growth inhibition properties. This is because the direction of flow, containing HPLC6 and its aggregates, to the ice particle surfaces can change as the ice particle grows, and thus the probability of interaction between HPLC6 and ice surfaces does not increase. In contrast to this, ultrafiltration after preheating the solution improved the ice growth inhibition. This may be due to the interaction between ice surfaces and many aggregates in the concentrates.

G133 The inhibition of ice growth for a plate with polypeptides based on antifreeze protein

2015 ◽  
Vol 2015 (0) ◽  
pp. _G133-1_-_G133-2_
Author(s):  
Kazuhide Arai ◽  
Masato Nishi ◽  
Takuya Miyamoto ◽  
Yoshimichi Hagiwara
Keyword(s):  
Antifreeze Protein ◽  
Ice Growth
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